FastQCFastQC Report
Tue 10 May 2022
EGAF00003650499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319497
Sequences flagged as poor quality0
Sequence length146
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT34970.26502523310018894No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC28110.21303572497701775No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG26630.20181932963849103No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG26380.1999246682637399No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT24030.18211485134107921No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC23580.17870446086652716No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT21370.16195565431372713No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA20930.15862105029416512No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG19920.15096661834017053No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA19690.14922352987539947No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18720.14187224374136506No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC18620.1411143791914646No Hit
CCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGC18400.13944707718168362No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT18220.1380829209918628No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17830.13512724924725103No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17580.13323258787249992No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA17530.13285365559754966No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA17380.131716858772699No Hit
CTCCCACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG17120.1297464109429578No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT16990.12876118702808723No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG16850.12770017665822658No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG16630.1260328746484456No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16240.12307720290383381No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA16010.12133411443906277No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG15840.12004574470423199No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15710.1190605207893614No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG15480.11731743232459035No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT15390.11663535422967994No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG15300.11595327613476954No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT15100.11443754703496863No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG14750.11178502111031705No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC14640.11095137010542654No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG14400.10913249518566545No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG14280.10822305772578489No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA14250.10799569836081475No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG14240.10791991190582473No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT13990.10602525053107358No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC13690.10375165688137222No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG13580.10291800587648173No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13540.10261486005652154No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC13280.10064441222678035No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13220.10018969349684008No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAG13210.10011390704185003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)