FastQCFastQC Report
Tue 10 May 2022
EGAF00003650503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382936
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC6370.16634633463555268No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6220.16242923099421314No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC5490.14336599327302735No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA5150.13448722501932436No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4990.13030898113522887Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA4910.1282198591931811No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA4500.11751310924018636No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT4460.11646854826916246No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC4440.11594626778365053No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT4390.11464056656987068No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC4310.11255144462782292No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT4220.1102011824430192No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA4070.10628407880167966No Hit
GTATAAGAGATCAGGTTCGTCCTTTAGTGTTGTGTATGGTTATCATTTGT3940.10288925564585205No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA3920.10236697516034012No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT3880.10132241418931623No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC3880.10132241418931623No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA3850.10053899346104832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)