FastQCFastQC Report
Tue 10 May 2022
EGAF00003650504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382936
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC5950.15537844443980195No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG5940.15511730419704597No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC5830.1522447615267303No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT5260.13735976768964006No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT5050.13187582259176467No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA4780.12482503603735351No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA4470.11672968851191845No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT4460.11646854826916246No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA4450.1162074080264065No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC4270.11150688365679906No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC4200.10967890195750726No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA4190.1094177617147513No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA4120.1075897800154595No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4120.1075897800154595Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC3920.10236697516034012No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA3900.10184469467482817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)