FastQCFastQC Report
Tue 10 May 2022
EGAF00003650505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383508
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6060.15801495666322474No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC6040.1574934551560854No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC5410.1410661576811957No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA4950.12907162301699052No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4840.12620336472772406Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA4780.12463886020630599No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT4620.12046684814919116No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT4610.12020609739562148No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA4530.11812009136706406No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT4360.11368732855637953No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA4210.10977606725283437No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT4140.1079508119778466No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC3980.10377879992073177No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC3930.10247504615288339No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG3900.10169279389217435No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA3890.10143204313860467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)