FastQCFastQC Report
Tue 10 May 2022
EGAF00003650506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383508
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6270.163490722488188No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC6150.16036171344535186No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC5560.14497741898474087No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA5140.1340258873348144No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT4980.12985387527769957No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA4660.12150985116346985No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA4500.11733783910635501No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT4360.11368732855637953No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT4280.11160132252782211No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA4250.11081907026711307No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC4230.11029756875997371No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA4210.10977606725283437No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT4040.10534330444214984No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4040.10534330444214984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG4000.10430030142787113No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC3970.10351804916716209No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)