FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2846
Sequences flagged as poor quality0
Sequence length146
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA80.28109627547434995Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATTGCCTTTGGTCTACAACTGAGGAAACAATATAGGGAGTCAGGTCAC70.2459592410400562No Hit
CCTAGGTAGCTGGGACTACAGGCGTGTGCCACCATGCCCGGCTAATTTTG60.21082220660576245No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50.17568517217146873Clontech Universal Primer Mix Long (96% over 26bp)
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG50.17568517217146873No Hit
ATTCTTTACCTTTCACTTCATCTTGCCCTTCATTATTGCAGCCCTAGCAG50.17568517217146873No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG50.17568517217146873No Hit
CTGTTAAGACAGTAAGCTAGAAAATCATAGAGGTAGCTCAACTCATTTGT50.17568517217146873No Hit
CTTTTGCTCTGTGCTTCGTCACCCACGTAGCTGTCTTTTTGTCCCATTCC50.17568517217146873No Hit
GTACATGGGAACGAAAAGAAGAAAAAGATCACTGCTTCCTGGTTTTGACC50.17568517217146873No Hit
AATATATGGAGTTAAGGTGTAATGACATTCCAGGTGTGCATTAGGAAGGT50.17568517217146873No Hit
CGTCCAGGGAGAGCAGCAGCAGGAAGAGCCTCAGGAGATGACCCTCAGGG40.14054813773717498No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT40.14054813773717498No Hit
GTTAGAAAAAGTCTTGCTGATACAAAAACAATACTGTTTTTGTAAATGGT40.14054813773717498No Hit
GGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCC40.14054813773717498No Hit
GGTGGCCAAGAGTCTCCGCAACATCGCGGTGGGTAAGGACCCCCTGGAGG40.14054813773717498No Hit
GTTGAAAGGTATATGGAGAATGGTCATTAGACGTCTCGACAGCCACCTGC40.14054813773717498No Hit
CTCTGCTGTCGCCCGTCCCGCGCGCTCCTCCGACCCGCTCCGCTCCGATC40.14054813773717498No Hit
GTCCATAAACTGGTTCTCCAAAATGTCTTCGCGAATCTGCTCCTTTTCTG40.14054813773717498No Hit
GGATAATTAGTATTCCTCTCCTTCTTCCTCACCCTCTCCTTCAACAGAAT40.14054813773717498No Hit
CCACAGCCCAGACCTGGCCATAAGGGACACCTTTGTGAATGCCTCTCGGA40.14054813773717498No Hit
AAGGGGGACGGCCCGAGACGGAGAAGGGAGAGAGTGGGAGTTTCCCAGCC40.14054813773717498No Hit
GGGTGGAGGTGGGGGACACAGGTGCGCAGCTGCACAGAGTCAGCAGCAGC40.14054813773717498No Hit
CTCCATGATGAACCGCAGCACACCATAGCAGGCCCTCCGCACAGCAAGCC40.14054813773717498No Hit
TTGCAGCTCTCTACCTACTTAGATCCCGCCCTGGAGCTGGGTCCCAGGAA40.14054813773717498No Hit
GGGGGCCGGGTCTGGCGAGACCAGGAGACGGACGGACCGTCCCCGCTTCC40.14054813773717498No Hit
CTCCTATACCATGTCATCAGTGTTCACATTTTCCAAATGAGTTGAAGTGC40.14054813773717498No Hit
CCCTACTGCTGCTACACAAGACCCTGAGACTGACCTGCAGGACGAAACCA40.14054813773717498No Hit
GTCCATCTCTTCCTCTCTGGTCCCCCTCTCCCTGCTCGGCCCACTTTCCC40.14054813773717498No Hit
TCCATGAGCAGTGGGAAAAGGCTAATGTAAGCAGTCCCCACCGGGAGTTC40.14054813773717498No Hit
CATTCTCACCTGTTGGCTGGTCTTCCAGAAGAAGCGTGTCTTCATAGCGC40.14054813773717498No Hit
CCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGG40.14054813773717498No Hit
ACTCTGGTCCTAGCAAATATTGATGATCTTTTTGACAGAGAAGAATTGTC40.14054813773717498No Hit
AATGATCGCTTGGCGGTCTACACCGACCGTGTGCGCTCGCTGGAAACGGA40.14054813773717498No Hit
GGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTA40.14054813773717498No Hit
GGAGACGCTGCAGCGCGCGGAACACCCAAAGACACACGTGACCCACCATC30.10541110330288123No Hit
GGCCGAAGGGGTGGCGCTGCGATCCCGCAGGGCAGCGACGCGACTCTGGT30.10541110330288123No Hit
CACCTCTACCGCAGTTATAGCAGGCATCCTCCTGAAGATCACAATCCTTG30.10541110330288123No Hit
GATCTCAAATGAACTGATGTTCTCCGGCAAATGTATTACATTTTCTCCAT30.10541110330288123No Hit
GCCCTCGGGGCTCCCAGACCTCAAGCTCCTCCAGGTGGTCTATCTGCACT30.10541110330288123No Hit
GGTCAGCGCGGTGGTGGTGCGCAGCATGGCATTGCTGCTCGGGCTCTTCT30.10541110330288123No Hit
GTCTACCGCCTCGGCTCCAACGACAAGGAGATCATGAAGGAGCTGATGGA30.10541110330288123No Hit
TCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCACGG30.10541110330288123No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC30.10541110330288123No Hit
GCTCTTGGTTCTGGGCTACTCCTTCAGGCCTTGCTCTGAACTGCAGTCAC30.10541110330288123No Hit
TCTCAGTGCTGTGCCCCCCCAGAGCCTAGAGGATGTTTCATGGGATCCCA30.10541110330288123No Hit
TCTGAATACAGCTGGTAAATGAAGTCGGTGGTGTAGTTTTCACTGCAGCT30.10541110330288123No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA30.10541110330288123No Hit
AGCCAATACATTTGTCCTGGGGCAAAATATGGCTTTCTGTAGGAAGCACC30.10541110330288123No Hit
TCTTAGAGGTGGGATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCA30.10541110330288123No Hit
TCTCTCAACAGACGTTCCCATTCCATAGCGATTATTCTCACAGATGAAAA30.10541110330288123No Hit
GACCAGACAGATGGCCTGGGCTTGTCCTACCTTTCCAGCCATATCGCCAA30.10541110330288123No Hit
CTCTTGATCCCTTCCATTAAATACTCAACTTTCTTGCTATCCTTGTCAGC30.10541110330288123No Hit
CCATTGACTGAAGACAGTCCCTATCCTAGAGGGGTTGAGCTTTCTTCCTC30.10541110330288123No Hit
GATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTA30.10541110330288123No Hit
GGTGAAGAATTACCACATCCCAAATCCATGTTACAGGCCACAGCAGAAGC30.10541110330288123No Hit
GTCTTGGGTGTCACAAAGAGGGCAGAGATGGTCAGCGTGAAGGCCTTGGA30.10541110330288123No Hit
GTACATGGGGAATCGTCTTCCGGCGCAGCCCCGTCCCTGTTTTTTGTGCT30.10541110330288123No Hit
CCTTTAAAAAGTCGTGGTTCTCGCCAGGCGCAGTGGCTCATGCCTGTAAT30.10541110330288123No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC30.10541110330288123No Hit
GATGAAGCACATGTTCCGCTTAGCTAGATAATAATGAGGTGGCACACACG30.10541110330288123No Hit
CAGTTCCCATCTAGCAAGGTGGCTTCAGGAGAGCAGAAGGAGGACCAGTC30.10541110330288123No Hit
CCTCACGCCCTGGTGCGACCTCCGCAGGTACGCTTCAACCTCCGCACGGC30.10541110330288123No Hit
GGGTTGAGTCAAGATGGCGGCCAAGGTGGCGAAGCAGCAGCCGCGGCGGC30.10541110330288123No Hit
TTCTTGTTCTCCACCAAGGTGGTGACGGTGTTAACTCCTGCTCGAAGGAC30.10541110330288123No Hit
CACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAG30.10541110330288123No Hit
GAGTACATGGGGCGGCCTCTTCGCTTTTGTGGCGGCGCCCGCGCTCGCAG30.10541110330288123No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC30.10541110330288123No Hit
GACCAGACCCAGGACACCGAGCTTGTGGAGACCAGGCCAGCAGGAGATGG30.10541110330288123No Hit
CCTGCAGGCAGAGCCGGCTTGGGGCCCAGCACCTGGAGCATCGCCTCGGG30.10541110330288123No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30.10541110330288123Clontech Universal Primer Mix Long (96% over 26bp)
ATCTTAGATTTGGTGTTCTCTAGCAGGCAGACTTAGTTTCAGTCTGGTGG30.10541110330288123No Hit
AGGAAGAAGAACAACTAAGGAGAAGGAAGAAGGAGAAGAAGAAAGAAGAA30.10541110330288123No Hit
CACTGTGCCCATCTACGAGGGGTATGCCCTCCCCCATGCCATCCTGCGTC30.10541110330288123No Hit
CTTGAGGCCCACAGCCTGGTGTGTTTCACGCACAGATAAGGTCCTCCCTA30.10541110330288123No Hit
GCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAACATATGCAGAT30.10541110330288123No Hit
ACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCACAGGA30.10541110330288123No Hit
GGGGACAATGAGATGTCACTGCTAGGTCTGGGCAGTGACAAAACCATACC30.10541110330288123No Hit
CGTGCACACGCACCCCACGCACGGCCACACGCGCACCCACGCTCGCTTGC30.10541110330288123No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA30.10541110330288123No Hit
GAGGAAGCAGGCGCATCTGGGTGTGGGCAATGACACGGATGACTTGGCAG30.10541110330288123No Hit
ACCTCCGCCTGGGCAACAAGAGTGAAACTCCGTCTCAAAAACAAACAAAA30.10541110330288123No Hit
GACTGATAGCTCCCAAGGCATCCCCCAGCTTGTGAGTAACATCAGTGCCT30.10541110330288123No Hit
CCACCCTCCTCTTCCCTCTGTGCCCTACTGCCTCTGCTGGCAGACAGGGG30.10541110330288123No Hit
CTCCGACTCCGAGCAGTCTCTGTCCTTCGACCCGAGCCCCGCGCCCTTTC30.10541110330288123No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCCACTTGGATAACTGTGGT30.10541110330288123No Hit
GGGTCACCCTGTCATGGTGCAGCTCCTCTGTCTCCCGGTCCAGCTTGGCC30.10541110330288123No Hit
GTACAGGACCAGCTGCTCCAGGAGGCAGCCCGGCTGCCGGACGCTCTCCA30.10541110330288123No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA30.10541110330288123No Hit
GGACGGGGCCCCCCATGCTGTCGGGGCCCAGGGCTGCTGTCCGCAAAGAG30.10541110330288123No Hit
CTGTACGCCTCTGGCCGTACCACTGGCATCGTGATGGACTCCGGTGACGG30.10541110330288123No Hit
CCCAGCTGCTGGCCCGGTTCGGCGCCGACGTGATCAAGGTGGAGCGTCCG30.10541110330288123No Hit
GCATGGGGGTTGGGGGTGAGGGACTTAAAAGTAGACATGCTACACCTAAT30.10541110330288123No Hit
GACCTGGGCTGTAGTTTTATTGTGCTTGGCTGCGATCGCCTTGATCCTGG30.10541110330288123No Hit
CTGAAGGGAAGAGGGTCTGTGGTTCTAGCTGCACACAAGGTCCCATTCAC30.10541110330288123No Hit
AATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTT30.10541110330288123No Hit
CAAGTGGCTGGCGCTGTCCAAGACACCATCGGCCCTGGCACTGAACCAGA30.10541110330288123No Hit
CAAGCTAACATGACTAACACCCTTAATTCCATCCACCCTCCTCTCCCTAG30.10541110330288123No Hit
AGGTAACAGTACGTCACCATTGGCTTCTGGCTCATTGAGTGATGGTGGGA30.10541110330288123No Hit
GAAGGCATAATTCCACAGGACATTCACAGTTGTGTGCTAGAGACAGAGAG30.10541110330288123No Hit
AAAATATGGTGTGAAGGTTGGCCTGACAAATTATGCTGCAGCATATTGTA30.10541110330288123No Hit
CCTGCTGCCTGGGCTTCATAGCATTCGCCTACTCCGTGAAGTCTAGGGAC30.10541110330288123No Hit
GTTTAGATGATCCCAATTTTGTTGGCAACATCCAAAGCATCGTAATCAGG30.10541110330288123No Hit
CCTCAGGGGTAGCAGAATGGCGGTTGCGGTGGCTGTACTGGTGGGGCTCT30.10541110330288123No Hit
AGGATACTGCGAGCAAATGGGGTGGAGGGGTGCTCTCCTGAGCTACAGAA30.10541110330288123No Hit
GATCTAGGTATTTAGGTAGCTTCTTACCATCATGAGACCAGATTTCAACA30.10541110330288123No Hit
GGTGCAATCTCGGCTCACTGCAACCTCCGTCTGCCAGGTTCAAGCAATTC30.10541110330288123No Hit
GGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTG30.10541110330288123No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA30.10541110330288123No Hit
GAGTAGGATCCAGGGTTAGTTTTTGTAGCCTCGGCTGGCCCCTCGGCCTC30.10541110330288123No Hit
GAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCC30.10541110330288123No Hit
GCTCAAAGCCGAACTTGTACTCATATTTGTTTCCAGGTCTCATGATCACC30.10541110330288123No Hit
CTCGCCGTCCAGCTCCCGCTGCAGGCCCTGCGCGCGGTCTTCCGCCTCGT30.10541110330288123No Hit
GGGCCGCTGTGTATGGGACATCTTCGGACTTGAGTGTGCTCAGGACCTGC30.10541110330288123No Hit
GCCTGGGGCCAGGCTCTGTGCTTGGAGGCTTCTGCTGTCCAGAGCCTCTT30.10541110330288123No Hit
GCATTATCAAGGGAAAGGCAAGACTGGGACGTCTAGTCCACAGGAAGACC30.10541110330288123No Hit
GCAGAGTACATGGGAAGTGGGGAGGAGGCGGTTGCGGTTAGTGGACCGGG30.10541110330288123No Hit
GTGGAGCATTGCCGGCAGCGAGATTTTCTTCCATTTGTGTGTGATGATTG30.10541110330288123No Hit
CGGTAGTTGAGCAGGCAGCTGAGGGAGGCCATGTAGAGGTCAGAGAAGCG30.10541110330288123No Hit
GCATAGAGGTCCTTCCTGATGTCAATATCACACTTCATGATGCTGTTGTA30.10541110330288123No Hit
CTTCTCAGCTGCCCCCAAAGCCTTGACCTCACCCTCCTCTTCATCACTGG30.10541110330288123No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA30.10541110330288123No Hit
ACCAGCTCCAGCTCCCAGGATCCAGAGAGTTTGCAAGACAGAGGCGAAGG30.10541110330288123No Hit
GCTTTGTGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGA30.10541110330288123No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC30.10541110330288123No Hit
GGCCAGGTTGTCCCGGTCACCAGGCAGGAGTTCTGTCCTTTGAACTCCAT30.10541110330288123No Hit
TCATAGTGCAGTGTGGCCCTGATGTGGGCCGCCCAGACCGTGGGCGCTTG30.10541110330288123No Hit
CCCTGAAATGATGTTTGGAGACAACGTTTTAAGAATCCAGCATGGGTCTG30.10541110330288123No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG30.10541110330288123No Hit
TTATAATGAGGAATAAGAATCCAGTGAAAGAAGAGTGGTCCCTACCTGTC30.10541110330288123No Hit
GTCCAGAAGGCACCACCACAGCTGCCCACTTCTGGAAGGTTCCATCTCCT30.10541110330288123No Hit
GAGCAGCGGAGGGGGAGCGTGCAGAGCCGCTGCGGCCCTCACAGTCCGGA30.10541110330288123No Hit
CCCCAGGAGCCCCGCAGAACAGGAAGGAGGCCAACCCTACACCCCTGACG30.10541110330288123No Hit
GCCTCCTTCCCAGCCGCGCGGCTTCCTCCAGACCTCTCGGCGCGGGTGAG30.10541110330288123No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)