FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2846
Sequences flagged as poor quality0
Sequence length146
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCACACCCCTACCCACTGGAAAGGAGTGTTGTAAGACTAGGTTTTGG70.2459592410400562No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG60.21082220660576245No Hit
GTGCTGGGTGCCAGGGCAGTGATCTCCTTTTGCATCCTGTCGGCAATGCC50.17568517217146873No Hit
CCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAA50.17568517217146873No Hit
GGGTACAGTGGCCTACGCCTATTATCCCAGCACTTTGGGAGGCCGAGGTG50.17568517217146873No Hit
ACACCACCCAGTGTGGAGCAGCCCAGCCAAGCACTGTCAGGAATCCTGTG50.17568517217146873No Hit
GAGCTGACTTCGCCAAGTGGCGTTGTGTGCTGAAGATTGGGGAACACACC50.17568517217146873No Hit
GTCTCATTATGTTGGCCAGGCTGGTCTCCAGCTCCTGATTTCAGGTGATC50.17568517217146873No Hit
CCCGCTCCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATA40.14054813773717498No Hit
AAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTTTT40.14054813773717498No Hit
ACCCTATATTGCATCTCCTGTATCTGCCTACCAGGTGCAAAGTCCTTCGT40.14054813773717498No Hit
CTCCTTCACATGCCCCTCGCGGTACAACCGGTTGCCCTCCTGGTGGATAA40.14054813773717498No Hit
GCAGGTAAGTGGTCAGAGTGCTGACTGCATACCAGTCGACGGCAGGAAGC40.14054813773717498No Hit
CTTCAAGCCTGTGCTGCCACGGTCAGGAGCCATCCTGCACTCCTCCCCGG40.14054813773717498No Hit
GTTCAGCAGAGCCTCCAGGTCCTTCAGCCGAGCCTGAGCAGCTATCAGGT40.14054813773717498No Hit
GAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAACCTCACCCGGC40.14054813773717498No Hit
ACACCGGACACACTCCCTCTGCAACGGCGACAGTCCCGGCCCAGTTCAGA40.14054813773717498No Hit
GCCCACCCCCTGCTCCCCTATGAGCACCACTCGGTAGTAGGTGTTCCCTG40.14054813773717498No Hit
ATAGAGATGCTGCTTAGGTTATAAATAAACGGCAGGTGTTTTCTGATATC40.14054813773717498No Hit
TTCCAGAAGTTCCAGTCTCTTGGGTTTGGTTGTGCATTCCTGCGACCTTC40.14054813773717498No Hit
GAAAAGGTGTACGGCAGTGGCGAGAAGGTGGCTGGCCGGGTGATAGTGGA40.14054813773717498No Hit
TCTCCTATGACGGCTATTACTTTCTGATCATACTCTGCACTAACCACACC40.14054813773717498No Hit
GTGGCTGAGAGCCCACATCCTCCCCATCACATTTTACCTCCGCAACCCTC40.14054813773717498No Hit
CTGTTGTACCGTGGTGACGTGGTTCCCAAAGATGTCAATGCTGCCATTGC40.14054813773717498No Hit
GGGTAGGCGGTAGTGGCGGAAGAGGTTCGGCGGCTGATGGCAGATCAGGA40.14054813773717498No Hit
AACTCAGATTGTGGGAGAGCTGCAGCTGCCCCACCTTGGCTTTCAAAGTT40.14054813773717498No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA40.14054813773717498No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG40.14054813773717498No Hit
GGCTGGCCCATTCCATCCGCCTGCTCCTGGAATACACAGACTCAAGCTAC40.14054813773717498No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA40.14054813773717498Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCGGGAGCGCTCCGCGTCGCGAGGCCGCGGCGCCGCGCGCTCCTCATC40.14054813773717498No Hit
GCCCTGGCCGATGCTCAGAACCCCAGCCTCTTTGTAAAGATTCTCATCGT40.14054813773717498No Hit
GATCCAGACAGAGTATTTGCGCTCCGGAGGGGCAATGATCTTGATCTTCA40.14054813773717498No Hit
GCTGAGCATTGAGGATTTCACAGCATATGGCGGTGTGTTTGGAAACAAGC30.10541110330288123No Hit
CATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCC30.10541110330288123No Hit
CGCCCCACCAGAAGTTGTCATGGACCCAGCTTTGGCAGCACAGTATGAGC30.10541110330288123No Hit
ATGCGGAAGTGGCCCCTCTCGCCCCAGGCTGGGCCCCAGGAGTTGGCCGC30.10541110330288123No Hit
CCCAAAGAGCTGGGACTACAGGTACAAGCCACCATGCCTGGCCTTTTTTG30.10541110330288123No Hit
TCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAG30.10541110330288123No Hit
TATCCAAGAAGAGGAAGTTTGTCGCTGATGGCATCTTCAAAGCTGAACTG30.10541110330288123No Hit
TCCTTGTTCTTCTCCTTCTTCTTCTCCTTCTCCTCCTCCTCCTTCTTCTT30.10541110330288123No Hit
CACCCCCCCTCAAGCGCAGGACCAAGTGCAGAGTGGACTCTTTCTGGATG30.10541110330288123No Hit
GAGACGGGGTTTCGCCATGTTGGCCAGGCTGGTCCCCAACTCCTGACCTC30.10541110330288123No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.10541110330288123Clontech Universal Primer Mix Long (96% over 26bp)
GAGAAACTGTGTTATGTAGCTCTGGACTTTGAAAATGAGATGGCCACTGC30.10541110330288123No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT30.10541110330288123No Hit
AAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCG30.10541110330288123No Hit
GGCTGATGCAGGACTCGGCTGTGGGGACAGGGCGAGGAATGGAAGGTGGA30.10541110330288123No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.10541110330288123Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGATCACCCAGCGCAAGTTGGAGGCGGCCCGTGCGGCGGAGCAGCTGAGA30.10541110330288123No Hit
GTTGTACAGAATGATGTAGCTGTCGCCTCCATAGAACTGTCCATATGTGG30.10541110330288123No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT30.10541110330288123No Hit
CCTGGGGGGTGCAGTGGCCTTAGGGCACCCACTGGGCTGCACTGGGGCAC30.10541110330288123No Hit
CTCCAGGTCAAGCAAATAGTTGGGGGCGAGCTCAATGGAGGAGCAGTTCC30.10541110330288123No Hit
CTCATGGCACATGGGACCTCAGGGGTAGCCTGTTTGCCGATCCCCCCAAG30.10541110330288123No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT30.10541110330288123No Hit
GAAGAGAATCAAGCCACGGAGGATAAAAGAACAGAAAGAAGTAAAGGAGC30.10541110330288123No Hit
GCTCTGGGTTGGTGAAGGTGATGAAACCAAAACCCCTGGACCGCTGAGTC30.10541110330288123No Hit
GGCTGGAGGATGCGGGCGTGCGGGAGCTGTGCCAGGGCCTGGGCCAGCCT30.10541110330288123No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC30.10541110330288123No Hit
AGCTGAGGAAGAATCTGAGTTCCCTTCTACTTCTATCTCTGCAGTTCTGT30.10541110330288123No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG30.10541110330288123No Hit
GCCCTGGGCACTGTTGCTGGACGCAGCCTGAGAGCAGCTCCCTCCTTTTC30.10541110330288123No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG30.10541110330288123No Hit
GTGCTCGACGAGGGCCTGCTGGTGCTCGACGGCGTGATGCCCGGCCTGAA30.10541110330288123No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA30.10541110330288123No Hit
GAGTACATGGGTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGAC30.10541110330288123No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG30.10541110330288123No Hit
GCAAGGACCAGAAGCCATGAGCTAAACTGCTGGTCTCCTATGACCACTAT30.10541110330288123No Hit
CATGCGGTGCATGGTCTCCATCAGCTCTCGCAGGTCGGGCTGGATCTCAT30.10541110330288123No Hit
CTCCTGGAGCTACAGATAAGCAACAACAGGCTGGAGGATGCGGGCGTGCG30.10541110330288123No Hit
GATATATAGGGACAGTACCTTGTTCCTTGTTGCAACATCTTTGAGGATAT30.10541110330288123No Hit
GGGTATGCCCTCCCCCATGCCATCCTGCGTCTGGACCTGGCTGGCCGGGA30.10541110330288123No Hit
GTATATACCTGCAGCCTGGATGGCATCGTGCGCCTCTGGGACGCCCGGAC30.10541110330288123No Hit
GTCAGAGAGAAAAGCTTCTTTGGGGGGAATGCAGGAAGCACATTGGCCCC30.10541110330288123No Hit
ATGCCAGGGTACATAGTGGTGCCCCCTGATAGGACATTGTTAGCATAGAG30.10541110330288123No Hit
GCACTGGTGCATGAAGGTGAAGTAAGGAGGCAGCTACTCCCTCCACAAAT30.10541110330288123No Hit
TCTTTGAGCTGCATCTCCTGAATCTCCATCTTCTCCTCATCCTTCATGGC30.10541110330288123No Hit
CTTCCTCACCCCTGCCTCAGGATGCCTGGCTCTGAGCCACCCCTGCTTTG30.10541110330288123No Hit
CTGAGACCCTCCGCAAAGCCGGCCAGGTCTTCCTGGAAGAGCTGGGGAAC30.10541110330288123No Hit
CCCTGGAACTCCTCCCATCCACTCATCAGGGCCCCACTCCCACTTTGGTT30.10541110330288123No Hit
CCCCGCTCGTTGGCCCGGGTATCCCAGCGCGGACCCACGCGATACGCTGA30.10541110330288123No Hit
GTCTGCTGGCATCCATGAAACCACCTACAACAGCATCATGAAGTGTGATA30.10541110330288123No Hit
GTGGTGGGTTGTGTGGTGGGTTCATCAGTGGTATTAGCTGTTATTTTGGT30.10541110330288123No Hit
GCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTT30.10541110330288123No Hit
TCCCCTGGAAGACAAGAATGTACACTGCTCTGTGAAATAAACCTGTAAAA30.10541110330288123No Hit
CCCTCCGTCCTGCTTTCTTGCCACTCTTCAGCACAGGCCACTTTAAGCAT30.10541110330288123No Hit
AAAAGGAGGTTGTGCTAAAGGGAAAGGAGGATCGATGCACATGTATGCCA30.10541110330288123No Hit
GCTCTACCCCACGCGTACTCCATGAGGCTCTGACAGCCCTCCTCGTAGGA30.10541110330288123No Hit
TACTTAGAGAAGATGGTCTCCACATCTGATTTCTTCACCAGAGCTGTGTT30.10541110330288123No Hit
GTGCAGACCCGCGTGTGGCGCAGGCAAGGACCCTCAAAATAAACAGCCTC30.10541110330288123No Hit
GCATTGGACAGGACATCCAGCCCAAAAGAGACCTCACCCGCTTTGTGAAA30.10541110330288123No Hit
ATCTGGAACACAGCAGCCAAGGCAAGGAGACCTCCAATCACTCTCAGGAA30.10541110330288123No Hit
GAGATGGTCTGGGTTAGGTCCGGGTGGGCTGGTCCCAATGGGTCCAAAGG30.10541110330288123No Hit
ATATATTGCAGTTCGAAACCAATGTGACATTTGCAGTAGTAGCTTCCAAA30.10541110330288123No Hit
TGGCAAGTCCATCTATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAA30.10541110330288123No Hit
GTACATAGTGGTGCCCCCTGATAGGACATTGTTAGCATAGAGGTCCTTCC30.10541110330288123No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC30.10541110330288123No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA30.10541110330288123No Hit
GGCGTGTGGAGAAGTTCGATCTGGTGCCCGTGCCCACCAACCTTTATGGA30.10541110330288123No Hit
GTATCAACGCAGAGTACATGGGCACCTCTAGCATCACCAGTATTAGAGGC30.10541110330288123No Hit
CTTATGCTCCTGCGCTTTCTTCATAAACCAGTTGGGATCTCCACGGTAGT30.10541110330288123No Hit
GAGTCTGCCATGAAGAAGATAGAAGACAACAACACACTTGTGTTCATTGT30.10541110330288123No Hit
ATTGTAGAGACACCACCCATGGTGGTTGTGGGCATTGTGGGCTACGTGGA30.10541110330288123No Hit
GACCATAGCAGGCATCGCCTCAGCTGTGATCCTCTTTGTTGCTGTGGTTG30.10541110330288123No Hit
ACACTCCTGCCAGGAACTAAGACCAAAATTCCTCTCGTTCTCATTGGGTG30.10541110330288123No Hit
GTTTCACTATTTTTGGCACCTTTCCATCGTTTACTTGCTGTTTCTCCTGT30.10541110330288123No Hit
GTCTGTAGTCATCACAGGCTTCCCTATTGAGCTCGTGGACAGGCTTAGAG30.10541110330288123No Hit
GTGTTGGAAGCTGCTCCAGGGATGTTGCAGCCCTAAGCACAGTGACAGGT30.10541110330288123No Hit
GTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCA30.10541110330288123No Hit
GGGTGCCAGGGCAGTGATCTCCTTCTGCATCCTGTCGGCAATGCCAGGGT30.10541110330288123No Hit
CCACAGCTCCCTTCAGGGCACCAAAAACTTTATTGCCAGTGGTAGTTCTG30.10541110330288123No Hit
GTGTCCCCATGGTCATGGTTCCCGCTACTGTGTCCAACAATGTGCCGGGT30.10541110330288123No Hit
CTTCGGACACCTGCGGCCCCTGCGAGCCGGCCTCCTGCCCGCCCCTGCCC30.10541110330288123No Hit
GTCTCACTCTGTTGTCAGGCTGCAGTACAGTGGTGCGATCTCAGCTCATG30.10541110330288123No Hit
CTCCAGGTCCTTCAGCCGAGCCTGAGCAGCTATCAGGTCACCCTCCTTCT30.10541110330288123No Hit
GAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCC30.10541110330288123No Hit
GGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGTAGAAGTAGAGG30.10541110330288123No Hit
GGCCTGAGCAGGGCAGGCTGGGTTGGACGCAGCTGGGCCCTGGCTTAGCT30.10541110330288123No Hit
ATCCTGGGCAAAAGGGACAAAGAGGGCAAATGAGATCACCTCCTGCAGCC30.10541110330288123No Hit
ATTTTTGCTATCATTGTGCTTGATATATTGGATGTAAAGTTCATCTATTT30.10541110330288123No Hit
CTATTATACATGCAACACTGAACTGGGGAACATGGCTTGGGGCCTCCAGG30.10541110330288123No Hit
GCCCATAGTAGCGTCAGGTCGCCGTCAGCCCTCAGGAAGCGCAGCAGCTG30.10541110330288123No Hit
ATCCTGTACTGCTTCATTTATTTATGCTGTTTGTGTGGGCCCTGTGAGTT30.10541110330288123No Hit
ATGCAGCACTTTATGAGATGACTTCTTCTCTTTCAAGTGTCATGAATTCC30.10541110330288123No Hit
TTCTGACCCTTGCCGGTCTGCTCGCGCCCGATGATCGCCGTCAGGATGCC30.10541110330288123No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC30.10541110330288123No Hit
GATTCTAGCCTCTAGCATGGCATATAGAGTCCTTCCCAGCCTGGCCTCCT30.10541110330288123No Hit
ACAGAAAACTCAACAGTGTACATTTAACCCAGTTTAGTGGCAAGTTCTTT30.10541110330288123No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA30.10541110330288123No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTAT30.10541110330288123No Hit
AAAAATGAGCTGGGCATGGTGGTGCTTGCCTGTAGTCCCAGCTACTCGGG30.10541110330288123No Hit
GTACATGGGGAGGTGGTCTGGAGCCTGCCGGGAGAGTGGTGGCATCTGAG30.10541110330288123No Hit
GCGGAAGTCAGAGAGTGAGGCCATCAGTCTATCCAGCTCCAGAGTGGCTG30.10541110330288123No Hit
TCCAACTTCTTCCACTTCGTCCTGGCCCTCTACGACAGACAGGGCCAGCC30.10541110330288123No Hit
GGTTAGGGCAAAGGGACAGGGCATGGGGCTGGGGTTCCCACTTTTTCCAG30.10541110330288123No Hit
CTCGAATGATGATCTGGGGGTGTAAACCTTCCTCCACATAGGGTTTCACC30.10541110330288123No Hit
GTCTCTAGCAGCTGCCGCTGAGCCGCCGGACGGACGCTCGTCTTCGCCCG30.10541110330288123No Hit
GCTCCAAGGACAGCTAGGACAACCAGGACAGCCAGGCCAGCAACGATGCC30.10541110330288123No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)