FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203
Sequences flagged as poor quality0
Sequence length146
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACAGGGTTTCACTATGCTGGTCTCAAACTCCTGACCTCAGGTGATCCT41.9704433497536946No Hit
CTATCAGTCAGCCTCGGAATCTCGCTTGCAGCCTCACCCTCTGGCTGCAG31.477832512315271No Hit
CCATAGGGCTATGATCTGCAGTAGTTCTGTGGTGAAGAGACTTGTTTCAT31.477832512315271No Hit
GGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCG31.477832512315271No Hit
CTCCACTGGTGTATGTTCATCAGAATCTGGTTCAGGATTCTTTGGAGAAA31.477832512315271No Hit
AGATTGAAGAGTTCAAATCTGACCAAGATGTTGATGTTGGATAAGAGAAT31.477832512315271No Hit
GGGGAGAACTGTGTGGCCAGAACAGAGGGGCCCTGTGGCTCCAGGCAACA31.477832512315271No Hit
CCCTCATCCTGCTACTCTCGGGGGCCCTGGCCCTGACCCAGACCTGGGCG31.477832512315271No Hit
GGAGTAAAGAGCCGAGGCCGGGCGGGGGCCGCCACGCATCAGGGCCCCCG20.9852216748768473No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT20.9852216748768473No Hit
ACATGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC20.9852216748768473No Hit
GGGTGGAGCAGGAGGGGCCGGAGTATTGGGACCGGGAGACACAGAAGTAC20.9852216748768473No Hit
ACTCAAGAGGTCAACAATTGGGATAAAATGCCATGCTGTGGGTGATGGAC20.9852216748768473No Hit
CAGATATCCTGTTGGCAACCTTGTGAGGCATGGCAGGGGAACTTTGCCGG20.9852216748768473No Hit
CCTTTCCGGTATAGCTGAAGGGGCTTCATGTTGATGGTCACGGTGATGGG20.9852216748768473No Hit
ATTTGACGCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG20.9852216748768473No Hit
CCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTTG20.9852216748768473No Hit
GTACATGGGAGTCGCTGGGTGCGTGGGGCTGCCTCGCCGCGTCTCGCCAC20.9852216748768473No Hit
CTCAGCTAAGAGCATCGAGGTGGCGCCGAGAGGCAAGGGGCGGGGACGGG20.9852216748768473No Hit
ACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGG20.9852216748768473No Hit
GGTCACCCCAGCCCCGATAACGGTCACCTGGTCATCCTTGCTCTTCAGGA20.9852216748768473No Hit
CCACCCACTCAGCTCACTCGGGCCTCTGGCCAACAGGGCATGGGGACATC20.9852216748768473No Hit
GCTTTAACCTGCTGAGTGCTCGCTTCCTGGTCTCCTTGGCGCGAACCTTC20.9852216748768473No Hit
CCACTGTGGAGAAGGATGAGTCCCATGCAGGCAAGGTGGTGCTGAGGAGC20.9852216748768473No Hit
GACCAGCACCATGGAGGAGGACAGGGTCCCAGTGATCTGTATTTGATGAG20.9852216748768473No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTC20.9852216748768473No Hit
CAAATGCAGTTTGACTTCAAGACCGTGGCTTTTCTCTTTCCACCAGCTGC20.9852216748768473No Hit
GCTGTGATGTGGGCACCACGGGGACGAACTTGGGGCAGTTGGCCATCTGC20.9852216748768473No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC20.9852216748768473No Hit
CTTTATTAGTGGGGAAACTCGCCTTGGTCTGGCAGAGACTGGGATCAACA20.9852216748768473No Hit
GGTACATGGTGGTGCCGCCAGACAGCACTGTGTTGGCGTACAGGTCTTTG20.9852216748768473No Hit
GTCATCTTCACCTCTTGTAGTGTGTGTGAGTGTTTTCCCAGGGGGGCCGT20.9852216748768473No Hit
CTGTGAGAGAGTGAATGTTCGTTGCTTTAAGCCACTACGTGTGGGTGACT20.9852216748768473No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAACCTCAC10.49261083743842365No Hit
CCTCCAGGTGCTTTGTGTCCCACAGCGCCACCTGCCGCTCACTCATGCGG10.49261083743842365No Hit
CCCTAGCGTGGTGTCATGCGGCTCATAAAACAGGGGCAGCAGCCAGGCAC10.49261083743842365No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC10.49261083743842365No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA10.49261083743842365No Hit
GGCATAACCAGTACGCCTACGACGGCAAGGATTACATCGCCCTGAAAGAG10.49261083743842365No Hit
GTCTTATAGTAGATGCAGCGGCAGTCGAGTACATGCACCAGCTACAGTGC10.49261083743842365No Hit
GGAACATGGTGGTGCCGCCAGACAGCACTGTGTTGGCGTACAGGTCTTTG10.49261083743842365No Hit
GTACATGGGGGAACTGCTCGCTGAGCACAGCTGCACAGTGCTGTCAGAAC10.49261083743842365No Hit
GTATTAGACTGGGAGGGCTGCTGTGATAAAGGCTGCAAACCCAGCAGTGG10.49261083743842365No Hit
ACCATGTCGAGCCTCGGCGGTGGCTCCCAGGATGCCGGCGGCAGTAGCAG10.49261083743842365No Hit
GGTGACGGGGAATCAGGATTCGATTCCGGAGAGGGAGCCTGAGAAACGGC10.49261083743842365No Hit
GTATGCATGGCACTCATATTCTCCAGCATCTTCCTTACTTAGAGGAGATA10.49261083743842365No Hit
CCCACTCACAGACTGACCGAGCGAACCTGGGGACCCTGCGCGGCTACTAC10.49261083743842365No Hit
GGGTGAGGTTTCCCGTGTTGGGTCAAATTAAGCCGCAGGCTCCACTCCTG10.49261083743842365No Hit
CCGCCTCCCCTTTCCAGAGAGCCAGAAAGAAACCTCGAAGTAGGGGGAAA10.49261083743842365No Hit
GGACAGCCCTGTCCCACTCACTCTTTCCCCTGCCGCTCCTGCCGGCAGCT10.49261083743842365No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC10.49261083743842365No Hit
ACAGTATGCAATCAACAGGCCTGGTACAATAATGTCTCCAAAACCCAATA10.49261083743842365No Hit
TTCCTGCCCCCTGCACCCTGCTCTCTCCCAGGCCTTGAGGTCAGTGTGAG10.49261083743842365No Hit
GCCGCCACTCAGCACTGTTCGTCAGTTAATGCGTTTGGCGTCTGTGAGCC10.49261083743842365No Hit
TCCCTACCCTTCCTGCCAAGCCAGGGACTAAGCAGCCCAGAAGCCCAGTA10.49261083743842365No Hit
GCTACAGATTGATCAAGTGCATGAACTCGGTGGAGGAGAAGAGAAACTCC10.49261083743842365No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG10.49261083743842365No Hit
CCGCAGGCCCCGGCCATGAACGGGCTGCCCTCGGCAGAGGCGCCGGGCGG10.49261083743842365No Hit
GAGGAAGAGTGACCAGAGCTCTCGATCTCTCCCTACCATGTGAAGATGTA10.49261083743842365No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAAGGCGACTATGCGCCTAGT10.49261083743842365No Hit
AAGAAATCCTCTATCTTCAGGCCCAGGATGTAATCCAGTTTCATCTTGCC10.49261083743842365No Hit
GGCCTGGACTCCATGAAAGACGAGGAGTACGAGCAGATGGTCAAGGAGCT10.49261083743842365No Hit
ACCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGA10.49261083743842365No Hit
GAACTAGGGTTGTGGTAAAGATTTGAGTGTCATTGGCATGAAGACAGATA10.49261083743842365No Hit
CTCCTGTATCAGCCTCCCGAGTAGCTGGGACTACTGGCGCGCACTACCAC10.49261083743842365No Hit
CTTTATTACAGTAGGATGATGGTCCTGGGCCACCAGCCTAAACCTTGCAG10.49261083743842365No Hit
GTATTGGGGCATGGCATCCTGCTCGTGTAGCCTTCTCTGTGGCACGCGCT10.49261083743842365No Hit
GGTGTAGGGGGGTATTTCCTCGAAAGTCTCGGAGCTCAGGATCACAGCCA10.49261083743842365No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGA10.49261083743842365No Hit
GGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTC10.49261083743842365No Hit
GCCCTGCTGAGTGACCTGCACGCCTTTGCTGGCAGCGCAGCCTGGGATGA10.49261083743842365No Hit
ATCATCGTCATCCTCCTCCACAGAAGTCCCTGAAAGAGCTGCAGGAAATG10.49261083743842365No Hit
GGATGGAGTTGGCGCAGAAGCCCCGCAGCACGCTCCTCCGCTCCGCCTGC10.49261083743842365No Hit
TGGCCACACAGCCCCTGTGCTAGACATCGCCTGGTGCCCGCACAATGACA10.49261083743842365No Hit
GCTCAGGTGAATGCAGATTTTCCCGGCCATCTGTGCTGAAGTCCCTGTGG10.49261083743842365No Hit
GAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTC10.49261083743842365No Hit
AAGATTAGGCCGATAGCCACTGTCCATGACCCCGTAGGCATAAGTGTTCC10.49261083743842365No Hit
GCTTAGATCTTGTGGCCAAGAGTTCAGACCGTGGCGAAGTGGAGAGTGAC10.49261083743842365No Hit
CCTTCAGTGCCTTCTGGCTGGCAGACAGGTGCTTGACATGGGCCACCTCC10.49261083743842365No Hit
GAGTACATGGGATGAGATCCCGGCCTCAGGGTGGACGCAGTGGTTCTGCA10.49261083743842365No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC10.49261083743842365No Hit
CTTTTAGGTGGTTTGGATTTGGAGCGGGAGCGCCTCCTGTTGTCGTGTCT10.49261083743842365No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGAC10.49261083743842365No Hit
CTTCCTGATACTGCCGCCAGATTTCTTCACCATCAGTTCATCAACCATGG10.49261083743842365No Hit
GGCTCGGCACGGGCACCAACCACTGCCTCCAACTGCCCCATGCTGCCTGA10.49261083743842365No Hit
GGTAAATACTGGTTGGAGTGGACGAGTTTAAGTCTTGCCTGTTCCTTTCA10.49261083743842365No Hit
CCGCTGTCCCCATCAGCAACCATGTTCTTGCAGGCTGGGGGCAAGGGATT10.49261083743842365No Hit
GTTTTACACTATTTCTGTAGAATGGTTTTCTGTAATTTCCTTAGGAGTCA10.49261083743842365No Hit
GAGATACACCTGCCATGTGCAGCATGAGGGTCTGCCCAAGCCCCTCACCC10.49261083743842365No Hit
CTTTGGAGTAGAAACCTGTGAGGAAAGGTATTCCTGCTAATGCTAGGCTG10.49261083743842365No Hit
CCAGCGATCACAGCTCCAAAGAGAACCAGGCCAGCAATGATGCCCACGAT10.49261083743842365No Hit
GTGCTGTTGTCCAGGATGGGCCCTATCTTCTGCAGAGACTTGGCCACATT10.49261083743842365No Hit
GTACTTCTCATCGAAGAGCCGCTCGTTGGTCTCGGAATGCAGCTGCTGGG10.49261083743842365No Hit
TTTCAGCGGAGCGCGGCGGCAAGATGGCAGTGCAAATATCCAAGAAGAGG10.49261083743842365No Hit
CACTGCCCCATTCCTAGGAACCTTAGCAGGTGCTACCTATGAGGAACAGG10.49261083743842365No Hit
GGACTACAGGTGCACACCACCACATCCGGTCAATTTTTGTATTTTTAGTA10.49261083743842365No Hit
GTCCAAGGAGCTGCAGATGAAGCTGGAGAAGCTTCGAGAGAAGGAGCGTA10.49261083743842365No Hit
GGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCG10.49261083743842365No Hit
GGCTGGAGTTGGCGCAGAAGCCCCGCAGCACGCTCCTCCGCTCCGCCTGC10.49261083743842365No Hit
TCTCTCGGCAACGGGACTCTGCCAGCTCAGCTCGTTCCTCTGTGCGTTCC10.49261083743842365No Hit
CGCTCAGCCCACACAGCAGGACAGTGGACGTGGAGATAGCCACAGTGGTG10.49261083743842365No Hit
GGGTTGGACTGGCGGCCGTGGAGTTTGTGACATACGAGGTGACACCCCTC10.49261083743842365No Hit
GCCGCAGGCAGACACCCGAGGCATCCCAGAACCGCACCGTGCCGTCCTCG10.49261083743842365No Hit
GGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTT10.49261083743842365No Hit
ATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCT10.49261083743842365No Hit
CCCTTCTGAGCGCCCGGCCTATCTGGCCCCGGAGCGCAGCCTGTAACGCT10.49261083743842365No Hit
GGCCTCGAGCTCCTGACCTGTGATCTGCCCGCCTTGGCCTCCCAAAGTGC10.49261083743842365No Hit
CCTTAATGTCACGCACGATTTCCCGCTCGGCCGTGGTGGTGAAGCTGTAG10.49261083743842365No Hit
CCCGGGATCTGAGGACAGGAGCCTGTGCTTCTTCCACAGGACAAGGTGAG10.49261083743842365No Hit
CCTGCGCAGTGGTCTTCGCTGCTGGGGGCCGCGCATAGCTCCGACTACAG10.49261083743842365No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.49261083743842365Clontech Universal Primer Mix Long (96% over 26bp)
AGGTAGACGAGGGCGCTGTGGAGTCCTGTTTTGGGGGGCTGTCTTGGGAA10.49261083743842365No Hit
AGATACTTGTTCCCAGGACCTTAACTCACGTGTGAAGCCAGGATTTCCTA10.49261083743842365No Hit
ACCCTGCGGAGATCACACTGACCTGGCAGCGGGATGGGGAGGACCAGACC10.49261083743842365No Hit
GGCCAGCGGGGGAGGGGCCTTCCTGGTGCTGCCCCTGGGCAAGACTGGAC10.49261083743842365No Hit
GTATGCCAAATGAAGTTCATTTATCTGGGCTCCATATTTCAAAAGAATGT10.49261083743842365No Hit
GCCCCGCACCTTCCTCCTCATGCCGGGGCCTGCTGCTCCCCAGGTCTCCC10.49261083743842365No Hit
GGTCTGGGGAGTTTCCTCACTAGCTTCAACTCCTACTCTATCTGTTTTCT10.49261083743842365No Hit
GACAAAGTGTGAGGCCACAGGGGCAGGTCTGGGGAGAAGATGGATGGGCA10.49261083743842365No Hit
TTCATGATCCTGCTGATCATCGTGTACTGGGACAGCGCAGGCGCCGCGCA10.49261083743842365No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT10.49261083743842365No Hit
GGCTGGTTAGACTTGAGAAGCAATTGACAATAAACTCTACAGAACTGGAA10.49261083743842365No Hit
GTTGTCGTCTCTTGCGGTGCCGTTGCTGGTTCTCACACCTTTTAGGTCTG10.49261083743842365No Hit
CTCCTGGACCTCCCTTCCGTCCTGGCCTCCCTGCCTCTCCAGCCCCTCCT10.49261083743842365No Hit
AGTGTCAGTTTCCTAGGAACCAGTGACAATGATCAGAGATAGGGGGACTC10.49261083743842365No Hit
CTCTCTCAGGAGTGCACGAAGTTCAAGGTCAGCAGCTGCCGGGAATGCAT10.49261083743842365No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG10.49261083743842365No Hit
GCCCAGTGCTGGGGCGTCAGCTAAACCTGAGCTTCCAAGAAGTCAGGTTG10.49261083743842365No Hit
GTGAAGGGGTTCTTGGTGGGGTATGGGATCTTCCGCGTCTTGGACCCAGC10.49261083743842365No Hit
GCTCTGTACCATGAAGAAGGGCCCCAGTGGCTATGGCTTCAACCTGCACA10.49261083743842365No Hit
ATTCCAAGATTCTCTGCACTTCTTTTTCTGTCCCTTAATTGGCCTCCTCC10.49261083743842365No Hit
TATTAATAAGTCATACAATCAGCCATATTTACAAACTGTGTAACTGCTCC10.49261083743842365No Hit
GATTGACACTCAAGAAGAAATGAAGGAAGGGAAAGCAATGGCCCCTGACA10.49261083743842365No Hit
CATACAAGCAGCCAGGATGTACTGGAGACCTCGACGGTCGGATTGAGGAC10.49261083743842365No Hit
CTAGGAAGAAGGCAGCAAAGGAGCTGAGGCTGCAAGGTGCAGAAGTAGTG10.49261083743842365No Hit
GCCAATAGGAGAGGGTCCTCTTGAGCAATGTCCCAAGGCAAGGTCTTGGA10.49261083743842365No Hit
GCCCTGTTCCCCGCGCTGCGGCCTGGCGGCTTCCAGGCGCACTACCGCGA10.49261083743842365No Hit
CCCCACAACCGAGGCCTCAACAACAAATGCTTCGATGACTGCATGTGTGT10.49261083743842365No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA10.49261083743842365No Hit
CTCTGAAACTGACAGGCCAATCCTCAGAAGTTCTTCAGCTAATTCCAGGA10.49261083743842365No Hit
GCAGTGGGGCAGGTGAGGCTTGGCCTGCCCAGCCTGTCCTGAGTGTCATG10.49261083743842365No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10.49261083743842365No Hit
TTGTTATTTTTCGTCACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCA10.49261083743842365No Hit
TTAAAAATACAGAAAGAAGGCCGGGCGCGGTGGCTCACGCCTGTAATCCC10.49261083743842365No Hit
CTCGCGGACATGGGCGCTGATGCAGGTGGTGAGGCCAAACACGGCCCCGA10.49261083743842365No Hit
TCTTTACACGCTGCAGGGTCTGTACTTGGTGTACCACCCCGTGCAGCTGT10.49261083743842365No Hit
TTACAGGTGTGAGCCACTGTGCCCTGCCCAGTCTGGCTTTAATGCACACA10.49261083743842365No Hit
ACTCCGGCCCCTCCTGCTCCACCCACGGCGCCCGCGGCTCCCCTCTCGGA10.49261083743842365No Hit
TATCAACGCAGAGTACATGGGATGGCGGAGCTGTACGTGAAGCCGGGTGA10.49261083743842365No Hit
CATCTGTAGTCCCAGCTGCTCAGGAGGCTGAGGCAGGAGAATCACTTGAA10.49261083743842365No Hit
ATTTAAAGATGTTCACGTTACGCTGGACCAAATTAAGACGGCTTTCTCCC10.49261083743842365No Hit
CTTCTGGACTGCGCGATGACTGGACGCAAGTAACTTCTAGGTCTGCAGAC10.49261083743842365No Hit
GGCAAAGACCGGTCAAGTTTTTTACGTGAATGGGCTGACACTTGGGGGCC10.49261083743842365No Hit
GGGAGAGGCTTAGGCAGAAGCCCTATTACTTTGCAAGGGGCCCTTCAGAA10.49261083743842365No Hit
CACCCACACTGTGCCCATCTACGAGGGGTATGCCCTCCCCCATGCCATCC10.49261083743842365No Hit
GTTTTGAGACAGAGTCTCGCTCTGCCGCCCAGGCTGGTTAAAAGATAGGG10.49261083743842365No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA10.49261083743842365No Hit
TGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGG10.49261083743842365No Hit
GCTGCGGCCGCAGCCATGAGTATGCTCAGGCTTCAGAAGAGGCTCGCCTC10.49261083743842365No Hit
GCAGGGACTGGCAGTGCAGCAGGACGCCCGTGGCCGCCACCTGCTCCAGC10.49261083743842365No Hit
GGTCCTGCCGGGTCGCTGGCTGTGACTGGGGATGTCTTGTTGATCCGGCT10.49261083743842365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)