FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203
Sequences flagged as poor quality0
Sequence length146
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCAGACCTTGGAAGTTTAAAGCGGTTTGGAGATCTTGGGAAATACTT41.9704433497536946No Hit
ATCCTGGGTTCACCCTTTGATGTTGGGCCATTAAATCCAGTTTGTCTTTG31.477832512315271No Hit
GCTCTATCCACGGCGCCCGCGGCTCCATCCTCTGGCTCGCGGCGTCGCTG31.477832512315271No Hit
GGAGGGAAGCGCGCGGCGGCGGCGGCACCGCCGCGGGCCTCGCCCTCCGG31.477832512315271No Hit
TCTGCCCGCTCCCCGCGGGGCCGCCGCTCTCGCTCGCACTCTTGCTCTCG31.477832512315271No Hit
GCCGGGACCTGACTGACTACCTCATGAAGATCCTCACCGAGCGCGGCTAC31.477832512315271No Hit
CTGCTGCTCAGCCCCATAACTGCCCTCAGAGGACGGGAATTCACACCTGC31.477832512315271No Hit
ATGTAGGGTTATAATAATGTTAACATGGACATGATCTTCTTTATAATTCT31.477832512315271No Hit
CTCCCGCCCCTACATGGCTTATCTTATGATCTGGGATCAGAAGTCTCTGA20.9852216748768473No Hit
GTGCAGGCCCAGTAGAAATACAAAAATAAACTCTGCGGTCCAGCTGTCCC20.9852216748768473No Hit
TGGCCTTCCTGTCTGAGGCAACCTCCTCCTCAGCCCACACCCCCAACTTC20.9852216748768473No Hit
CCTCAGATCCATTGAACTAGGTCTGTCCCAATGTATGGGATGGCGGATAG20.9852216748768473No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC20.9852216748768473No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG20.9852216748768473No Hit
GGGGTGAGGCTTCCTGTGGCCCAGCCCGTCCCATTCACCTGTGGATTTGT20.9852216748768473No Hit
GACGGGCCCTCCCAGATGGCCCTAGAAGATCTGGCTATGTTTCGGTCAGT20.9852216748768473No Hit
GAGCGGCTTCTTTACTTGGAAGAAGGCCAGAAAGACTCCCTGATCGACAG20.9852216748768473No Hit
AACTCATCTTCTAACTTCTTCTTGAGCTGAAGATACTTGGACTTGTAGCC20.9852216748768473No Hit
GTGAGCACCCAGGCTCAGGGCACCTGTACAGAGCAAGAGGATAGCTGAGA20.9852216748768473No Hit
CTCCTGCTTCAGCTTCCCGAGTAGCTGGGATTACAGGCATGTGCCACCAC20.9852216748768473No Hit
GCCTGGTGGCCTCGGCTCAGCAGCGGCAGCCGCCGGCCGGGCCGGCGGGC20.9852216748768473No Hit
CCTTTCAGCTGTGACTGGCGGAAGGTGGCCGGCGCCATCTCACCCATCAA20.9852216748768473No Hit
GGCCCGGAGAGGGCCCAGCCCGCCCGGGGCAGGATGACCAAGGCCCGGCT20.9852216748768473No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA20.9852216748768473No Hit
TATCAACGCAGAGTACATGGGGGAAGAAGAGGAGGAGAATGACTCAGGAG20.9852216748768473No Hit
GGGCGCAGCCGGCTGCCAGCAGCACCCTCCGCACCGACAGGCGCATGCTG20.9852216748768473No Hit
GGTCACGTGTGTCTTTGGGTGTTCCGCGCGCTGCAGCGTCTCCTTCCCGT20.9852216748768473No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGG20.9852216748768473No Hit
ACCCCAAGCTCATCACCTGGTCTCCGGTGTGTCGCAACGATGTTGCCTGG20.9852216748768473No Hit
GCCGTCCTCCTCCCAGCGTGCCTGGCATGGTCGCAGGGGAGCGGGTGCCT20.9852216748768473No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG20.9852216748768473No Hit
CTTTAGTGATGTCCAATTCAAGATGAGTCATAAGAGGATCATGCTGTTCA20.9852216748768473No Hit
GGTTGTGACAACGTGATCATGGTGTGGGACGTGGGCACTGGGGCGGCCAT10.49261083743842365No Hit
ATCCTGATCTTTACTGTAATGAGGACAGTTGGCAGTTACCTATTCATGCA10.49261083743842365No Hit
AGCTGCTCATATTCATCTGACACCATGTGGCCACAAAGCGGAAACTCATC10.49261083743842365No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT10.49261083743842365No Hit
CCTGGGGTTGAGTGTCCTGGGCCTGAGTCTGCAGCCTCAGCCATCTGTTC10.49261083743842365No Hit
AGCCCTCCTCCTGCTGCTCTCGGGAGGCCTGGCCCTGACCGAGACCTGGG10.49261083743842365No Hit
GCTTTGGGGACCAGAAGGCTCAGATCAAGACATCGGCAGGATTGGCTCCT10.49261083743842365No Hit
ATCAACGCAGAGTACATGGGAGAGAGCGGCGCCGGCCTCGGCGGCTGAGG10.49261083743842365No Hit
GCGCCTGGGTCCGCCGGCCTGGGTCTGCAGCCCGTATGAGAACTGCGGCG10.49261083743842365No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG10.49261083743842365No Hit
GGGTTTCGCCATGTTGGTCAGGCTGGTCTTGAACTCCTGACCTCAGGTGA10.49261083743842365No Hit
GTTCCAGGCGGCCGAGCGCCCCCAGGAGTGGGCCATGGAGGGCCCCCGCG10.49261083743842365No Hit
ATCCCGGGCTGCACACGCAGTCGTAGCTCCCCTCTGTGTTCCAGCAGTCC10.49261083743842365No Hit
GGATAGTACTCTCACTGAGGTCACTGTCTGAGTCAGAGCTCCAGCCCTCG10.49261083743842365No Hit
CTAGGACTCATAATAGTTACAATCTGCATCAACCAACCACACCTAGCATT10.49261083743842365No Hit
GCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACACCTTTTTG10.49261083743842365No Hit
CCATAGTCCATGTTCCTGTCCATTATATTACACTGGGTGAGACTACCTAG10.49261083743842365No Hit
GGACGGTCCTTCTCAGCTACGACAGTGCCTTTGCGGGGCTCGATGATGCG10.49261083743842365No Hit
CTGTGATGGTGAGGGATGATCAAGTCCTCCTTGATGTACATGAGCTGCTC10.49261083743842365No Hit
GACGTAATGGGGTCCTGGCCATCCCACAAGTGGTGGTAACCCTGGTGGTT10.49261083743842365No Hit
GGTTTTTCTTGCAGGTAGTTCTGCCAATTTCCACCTCCAGCATATATTTC10.49261083743842365No Hit
GCCCGATGTTCTGGCCGACGTATTTTTCTGGCATCTTCAAAAGGCTGAGC10.49261083743842365No Hit
GTGGTTGGGGGGGGCCGTTCCCGTGGTGAGCGGGGTACACATGGACTTGG10.49261083743842365No Hit
CTCACCTCCCTTGCCTGGGTCAATTAGAGAAAGCTTGTCGGCCAGGCATG10.49261083743842365No Hit
AACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGC10.49261083743842365No Hit
CTGCAGCCCCGCTGGACCGACTTCCCCGGCCGGGTCCTGGACGTGGGCTT10.49261083743842365No Hit
AGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGT10.49261083743842365No Hit
ATCCACGCCAGCCGCACCATGCCGCTCCTAAGCCTGCACAGCCGCGGCGG10.49261083743842365No Hit
GGCTATGGCGCCGAAGGTTTTTCGTCAGTACTGGGATATCCCCGATGGCA10.49261083743842365No Hit
GGACCACGCTCTGAAGACCATTCATCCTAGCAAGCCCCTTTGTCTAGCAA10.49261083743842365No Hit
GCGCCGGGCGTCGCGCGGGGACCAGTCGTAGCCTCTCACGCTCTCCTCCA10.49261083743842365No Hit
CCCCATCTCTGACCATGAGGCCACCCTGAGGTGCTGGGCCCTGAGCTTCT10.49261083743842365No Hit
GGTCAGCAGTATGGCCATTTAGATGATGTAGAAGTAGTGCAGCAGGTGGA10.49261083743842365No Hit
CAGCTACCCGGTGTGCGGCAGCGACTGCACCACCTACCCGAGCGGCTGCC10.49261083743842365No Hit
GCTCTGAGGCCCCAGAAGGAGCAACCCTGACGACGCCTTGATTTCTGCCT10.49261083743842365No Hit
TGGCCTTCCTGTCTGAGGCACCCTCCTCCTCAGCCCACACCCCCAACTTC10.49261083743842365No Hit
GTCAGAGACAGCGTGGTGAGTCATATGCGTCTTGTGGGCGTCCGTGCGAG10.49261083743842365No Hit
GACTACACCGCGGCGGCAAAGCCAAACGCAAAAACTACCTCACCGCGCGC10.49261083743842365No Hit
GGAGTGCAATGGCATGATCTGGGTTCACTACAGCTTTGACCTCCAGAGCT10.49261083743842365No Hit
GGTCTCTGTACGGGCCAGTTTCTCTGCCTTCTTCCAGGATCAGGGGTTAG10.49261083743842365No Hit
CTGGAGGCGTTGGGGAGCAAGACTATGAGGTCCTTCTGGTCACCTCATCT10.49261083743842365No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT10.49261083743842365No Hit
CCCACATTCTCTGTGTGGTTCCCACCATCTAAGTCACTCCAACAGTTGGA10.49261083743842365No Hit
GGGCGGCGGACCCTGGGCCCCGTCAGTCTTCAGCAGCCAGGTCTACAATG10.49261083743842365No Hit
GCCTTGTGCACTGGCTGAATGCTTGGCCCCTGGGCTGTGATGTTCCCGTT10.49261083743842365No Hit
GGCCCAGTGTCAGCATGGCCGCCCCAGTGCCCACGTCCCACACCATGATC10.49261083743842365No Hit
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT10.49261083743842365No Hit
CCTTGGTTGATTCCCCAGGGTACGGCAGGGCCTTGGGAACCTGGGTGGGT10.49261083743842365No Hit
GCTGTCCCCAGATGCTCTGCCTCCCGTTCCAAAACCCTCCTCCTCCTCCC10.49261083743842365No Hit
AATTTACCCTGGCCAAGATCCGCAAGGCCGCCCGGGAACTGCTGACGCTT10.49261083743842365No Hit
GGCATAAACATGGCTCATTGCGGCCTCCATCTCCTGGGCTCAAGCAGTCC10.49261083743842365No Hit
CACGCAGACATCTGGGTCAAGAGCTACAGCTTGTACTCCAGGGAGCGGTA10.49261083743842365No Hit
CCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTT10.49261083743842365No Hit
GCATGGAGGGGAAGCAGCATGGGGACGTGGTGTCCGCCATCAGGGCTGGC10.49261083743842365No Hit
GGAGAGGGCAGCACTCCTGCCTTCTGAGGGGATCTTGGCCTCACGGTGTA10.49261083743842365No Hit
CAGCAAGCCCTCCTAGGAGTTTGTAACGCAGAGACTCTACCTGGGCAATG10.49261083743842365No Hit
TCCTCATTCTCCTCATCCATGTGACCTTCTCTGGCATTCGGGCATTGGCT10.49261083743842365No Hit
GGACACGGGGGTGGGGTGGTCTCCCCACCTCCTCACATTATGCCTACAAG10.49261083743842365No Hit
CTGCAACCTCTGCCTCCCAGGTTCAAGCGATTCCTCTGCCTCAGCCTCCT10.49261083743842365No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.49261083743842365Clontech Universal Primer Mix Long (96% over 26bp)
CTCCACCTCCTCTCTCACAGCATACATCAGATGATTCTTCACCAGATCCA10.49261083743842365No Hit
GAGTGGGCCCATGCCACAATCATCCCCAAGGTCTTGGCCATGTCCGGAGA10.49261083743842365No Hit
CCTTTCACCTGATGATGACCTTTGGGCCATCGTACAGGCATGTGTAAACT10.49261083743842365No Hit
GTGGTGCACCTCGCCTGCATCTGTCACTCATTCCACTACATCAAGCGCCT10.49261083743842365No Hit
AGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCACCCGG10.49261083743842365No Hit
TTCCAGAAGAGCAGGAGATGGACTTCCGGCCTGTGGACGAAGGGGAGGAG10.49261083743842365No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA10.49261083743842365No Hit
GGCCTGTGCCATCAGTATCTTAATGAAGGACTTGGCAGATGAACTTGCTC10.49261083743842365No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAA10.49261083743842365No Hit
GGCTGGAAGAGTGCCTCAGGGCAGCGGAACCGCTCATTGCCAATGGTGAT10.49261083743842365No Hit
GCTGGCCGCCCCTCGGGCCCTGAGTCCGAAGACCCGCTAAACAGGGCCCG10.49261083743842365No Hit
ACTCTAGACTGATACCTCATCATGAAATTTCTGCAGACGTCGCGCCATCG10.49261083743842365No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA10.49261083743842365No Hit
ACACTGAAGTTGCCCAACTTCAAGTCATGTGTGATACTTCTAGGCCTTCT10.49261083743842365No Hit
CTGTTCTTATAGCACTGCATCTGGTCGAGGATATATGTTGCTGAAGGTTG10.49261083743842365No Hit
CTCCTGGAATTAGCTGAAGAACTTCTGAGGATTGGCCTGTCAGTTTCAGA10.49261083743842365No Hit
GCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGG10.49261083743842365No Hit
TCACCATAGTGGACAAAGCCACCCAGAGGGTTGATGCTCTTGTCAGATAG10.49261083743842365No Hit
GAGGGAGGATGACAGGTCCTGGCAGAAGCTCAGGTCTTGGCACAGGTGTT10.49261083743842365No Hit
GTCCATCTTAGGCCTGGTATGGAAAGTGTTGCCCTTCAGGGAAATTAATG10.49261083743842365No Hit
GGGTAATTATGGTGGGCCATACGGTAGTATTTAGCCTAGTTGGCAAGCGA10.49261083743842365No Hit
GTCCCATGCTCTGGAGCAGGGTCATGAGCCTCAAAATCTCAGGTCTGAGG10.49261083743842365No Hit
GGTTGGTTTCTCCATGGCAAGATAAATCCAGGCGTTATTCTGAATTGTTT10.49261083743842365No Hit
AACCACCGTGGACACCTTCTTTGCCCATCAGCAGGACTAGCGTCTTGTCA10.49261083743842365No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC10.49261083743842365No Hit
GCTCTCCTCAAGGGGCCAGTCATGAGCCGGGCTTTCGAAGAGACCAAGCA10.49261083743842365No Hit
GTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC10.49261083743842365No Hit
GAATGCAGGCTGTACTATCTGCGAAATGGAGAACGTATTTCAGTGTCGGC10.49261083743842365No Hit
CTCGTAAGTCTCCTGGTTCCTGGTGCTCAGGCCCGTGTAAACACCATCTG10.49261083743842365No Hit
GAGTACATGGGTCCGCGGGAGGCCAGACGGCTGAGGGTGCCGGCAGCGGC10.49261083743842365No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC10.49261083743842365No Hit
CCCCACAGCTGCTCCAAAGTCTTTCCATCCAGTCCCAAGCATATACAGAG10.49261083743842365No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA10.49261083743842365No Hit
GGACAGGCCCCTGGGACCTCCCCAGTGCAGGTTCAATGAAAGCGGTGACT10.49261083743842365No Hit
GGTCCAGGCTGTCCACGCCCACGAACCAGTCGGGGTTGGGCACGATGCGC10.49261083743842365No Hit
GAAATCACTAGAGCTGTGATGGGCACATAGTCACCGCAGCGGTGGAGAAC10.49261083743842365No Hit
TCATTGGTCTTTATTGTTTTAGGAAATCCTGGCTTCACACGTGAGTTAAG10.49261083743842365No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG10.49261083743842365No Hit
GTACATGGGGTATGCTGATATGTCGCGATCTATGTGGTGACTATGAGATC10.49261083743842365No Hit
CGATATTGCTGTCATTGTGGGGTGGAGATCACTAGAATGTCAGGTGAGGA10.49261083743842365No Hit
GCCTATGATGACGATGTCATGGAGAACCGGGTTGGCCTGAACCTGCTTTA10.49261083743842365No Hit
GCCTAAAGCGTTGACCCAGTTGTGAGCAGCTCACAGGCCCTGCAGAAGGA10.49261083743842365No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTT10.49261083743842365No Hit
CCAAAGAGAACCAGGCCAGCAATGATGCCCACGATGGGGATGGTGGGCTG10.49261083743842365No Hit
GTGCAGGACTGAGTCAGGACTCCCACGCTGGCCAGGCAGCCCTGCTCACA10.49261083743842365No Hit
CAAGGCAGCTGTCTCACACTTTACAAGCTGTGAGAGACACATCAGAGCCC10.49261083743842365No Hit
ATGCAGAGGAGCGAGCTGAGCGCCTCCAGCGAGAAGTTGAGGGAGAAAGG10.49261083743842365No Hit
AGCTTCCGCAGTTTCTTAGCTCCAATTTTCCCCGACAGGTGAGTTTCCGC10.49261083743842365No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC10.49261083743842365No Hit
GGTGGCTTGGTTACTGTGACTGTAGCTGGAGTGGCACAGGTGACCCAGGC10.49261083743842365No Hit
CTACAGGACCTGTCTGACGATAGTCCAAAAACTGAAGAAGATGGTGGATA10.49261083743842365No Hit
TGCCTGGCCTGGTCGCAGGTAAAGCGTCACTTTTTGTGGGGACAGCTGCT10.49261083743842365No Hit
GGCCTGACACAATATCCCTGTTCACTTTGAAGTGAATTTTGACTCTATAT10.49261083743842365No Hit
GCTGAAGATGAGTGGGGTAAAACCCCAGACGCCATGAAAGCTGCCATGGC10.49261083743842365No Hit
AGTTGCTTGGTTACTGTGACTGTATCTGGAGTGGCACAGTTGACCCAGGC10.49261083743842365No Hit
CATATCCACCATACCCAACAGAAACCCACCAACTAACAGGGCATACACCT10.49261083743842365No Hit
CCGCAGCCTCGTGCCTTACGGTCCCTTTCCTTGCAAAGCGATTACCAGAA10.49261083743842365No Hit
TGCCAAGACAGCCCACAACAACAACTCCAGCCGGTTTGGGAAATTCATCC10.49261083743842365No Hit
GGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCT10.49261083743842365No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG10.49261083743842365No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCAGC10.49261083743842365No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC10.49261083743842365No Hit
GAATTCAGCTCCTCGCTGGTGTCACTGGAGGCGGATCTCACCAGGGCTGG10.49261083743842365No Hit
GTCAGAAGGATTCCTATGTGGGCGACGAGGCCCAGAGCAAGAGAGGCATC10.49261083743842365No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG10.49261083743842365No Hit
AACATACTCTCACCCAAATTTTCTCCTCCCTGTCTGACCATTCCCCAGTA10.49261083743842365No Hit
GTGTCATCTGCCACTGAGGCTGGTGCGGCGGCAGCCACCACTGCACTCTT10.49261083743842365No Hit
CCCGTACAACAAGCCCCGTGTCATGAGTGTACCTATGTAGCAAACATTCA10.49261083743842365No Hit
GACCTTCCCTATCTATGCGTCTCATCTTTGTCTTGCCTCACTGTGCTCCA10.49261083743842365No Hit
GGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAA10.49261083743842365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)