FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length146
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCAGCATGAACAGCGCAGTGATACGGTTCAGCTTGTTGATTTCGATA215.384615384615385No Hit
ACGACAGGCGCTGGCGAAATTGCGGCAGCAAGGGCGCGAACAGGTGGATG215.384615384615385No Hit
GCTCCGATATGTATGCCTTGGTCGCGGCCTTAATCACCGGGTCGGCCAGG17.6923076923076925No Hit
AACTCGAGGAGCGGGCCCGCCGCCGCGATCTGCTCGTCGGCGACGACGAG17.6923076923076925No Hit
GTATCGGATGACCAAAAAAGTTGTCGGGCGCATGCACAAAAAGAGAGCGT17.6923076923076925No Hit
GTCGAGGTAGACGTAGTCGCCGTTCAGCGCGTCGGACTGGGTGCCGAACT17.6923076923076925No Hit
GGATGCCGATCAGAGAGCCTTCCCCGATCGTGCAGCCATGGAGCATGGCC17.6923076923076925No Hit
TTCGAAAAGCTTGTCGTGGGGAATAAAAACTCCGAAAAGAACGATGTGTT17.6923076923076925No Hit
GGCGACGCCTTGGCCGGTCCGTTGGTCGAATTCAACCGTTCGCCCCGGGT17.6923076923076925No Hit
ACTTCAACTGGTTCGGCGACACCGTGCCGGCGGTGATCGCGATCATGGTG17.6923076923076925No Hit
TCCTTCTGTAAGGTTAAAAGAATGCTTTTATTTGCTGCTGTCTCTTATAC17.6923076923076925No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)