FastQCFastQC Report
Tue 10 May 2022
EGAF00003650676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644522
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG16270.25243513797822265No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG12410.1925457936269049No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT12390.1922354861432193No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA11410.17703041944262568No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG11190.17361703712208426No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC11190.17361703712208426No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC11040.1712897309944424No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA10480.16260112145124606No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT10350.16058412280728973No Hit
GGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG10070.15623981803569156No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT9920.1539125119080497No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG9870.15313674319883572No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA9700.15049912958750827No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG9680.15018882210382267No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT9400.1458445173322245No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT9060.14056929010956956No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT8950.13886259894929887No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC8680.13467344791954347No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA8650.1342079866940151No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC8090.12551937715081876No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA7810.12117507237922058No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT7680.11915807373526427No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG7490.11621015264025122No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA7410.11496892270550889No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG7380.11450346147998051No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA7360.11419315399629493No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC7260.11264161657786702No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT7180.11140038664312468No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT7120.11046946419206792No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA7080.10984884922469675No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC7020.10891792677363998No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA6930.10752154309705488No Hit
CCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTTG6890.1069009281296837No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG6880.10674577438784091No Hit
GCTATAGGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTAAT6880.10674577438784091No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT6820.10581485193678415No Hit
GGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCC6770.10503908322757019No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT6660.1033323920672995No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA6630.10286693084177112No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC6580.10209116213255715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)