FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length146
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTCTGCTGTCCCGCAAGATGCGGATGCTGCTGGCGCTCCTGGCCCTC34.054054054054054No Hit
CCTCCAGGCTGAAGCCGCGGCCGGCGCGCACCTTCGTGTGGTACCGAACC34.054054054054054No Hit
ACCGAGGAGAGGCTGAGCGTATCTTCATTGAGCTCAAGTTTACTGTACGT34.054054054054054No Hit
GCCTGCAGCATTGCCGTGGATGCCACCCTGAGAAAGAAAGCTGAAAAGTT22.7027027027027026No Hit
GGCAGGGAAACTGCTGGTGAATTCTGGAGACCTCGGCTGGGGGGCAGCTC22.7027027027027026No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA22.7027027027027026Clontech SMART CDS Primer II A (96% over 26bp)
AGATAGAACCTTCCAGAAGTGGGCAGCTGTGGTGGTGCCTTCTGGAGAAG22.7027027027027026No Hit
CAGTTTCCGCAGGTTCACTCGGTCAGCCTGTGCCTGGCGCTTGTACTTCT22.7027027027027026No Hit
GAGACCATACACCGTGATCCTTATTGAGAGAGCCATGAAGGACATCCACT22.7027027027027026No Hit
TGTCCTTGAGCTTACTGTCATCAATCAGGTCCTCTGCATCAGAGAAGTTC22.7027027027027026No Hit
GGCATGAAGCATCTCAGACTCCTTGGCAAAAAACGGAGTCCGCAGGCCGC22.7027027027027026No Hit
ACTGGATGCTGCGCTTGGTTTTGATGGTGGCAATGGCAGCATTGACATCT11.3513513513513513No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC11.3513513513513513No Hit
GAGCACTTCTGCGAGGGTACAGGGGTGGAAGTGGGTCTTATTGAATGGTC11.3513513513513513No Hit
GCTGGGTGATGGTCTGTGGCTCCTTGCCATCTCCACAGCTGCAGCGTGAA11.3513513513513513No Hit
AGGCCGAGTTCTATCTGAATCCTGACCAATCAGGCGAGTTTATGTTTGAC11.3513513513513513No Hit
CCCCATCTCTGACCATGAGGCCACCCTGAGGTGCTGGGCCCTGGGCTTCT11.3513513513513513No Hit
AAGCCACTGTGCCCGGCCTCTTATGTACAATTTCTGATAGGCTGGGGAGT11.3513513513513513No Hit
GGGGACCCTGCGCGGCTACTACAACCAGAGCGAGGACGGTTCTCACACCA11.3513513513513513No Hit
CATCGAACTGGTAGAGGGGCCAGAGAACAAGGGCGGTGGCATAGAGGAGG11.3513513513513513No Hit
GGCCTCAGTGATGCCTCCTGATCTATCCATAGGCCTGGAAGATCAGCACT11.3513513513513513No Hit
AGACGGGGTTTCACCGTGTTAGCCAGGATGGTTTCGATCTCCTAACCTCA11.3513513513513513No Hit
CATTAACACTGGCACCTACTCTGTGCCAGGCAGTTCTCCAGGTGACAGGG11.3513513513513513No Hit
AAGTGGGAGGAAAGGGCACTGCCTGAGGAGTCCGGGAGTCTGGTCATCCC11.3513513513513513No Hit
CCCTGGACTTCTCCACTGGCTTACGACATGAGAGCTCAATATGCTCCTTA11.3513513513513513No Hit
GGTTATGCCCGCGGAGGAGGCGCCCGTCCGGCCCCACGTCGCAGCCGTAC11.3513513513513513No Hit
CACAAAGCCCATGGAAAGGAAATGTTAATCCTGCCTTTTTCATGCTAATC11.3513513513513513No Hit
GCGGAGGGTGGCGATGGCCTGGGCAGCGCGCTGCGCCCCAGCACCAGCCG11.3513513513513513No Hit
GGAGTGCAGTGGCGTAATCTCAGCTCACTGCAACCTCCGCCCCCTTGGTT11.3513513513513513No Hit
GCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCCA11.3513513513513513No Hit
GGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCG11.3513513513513513No Hit
TCCCAGCACTTTGGGAGGCTGAGGCGGGCAGATCACGAGGTCAGGAGTTC11.3513513513513513No Hit
GTAGAGCATAAAATAGAATGCAGGTGTGTCAGTCGATGCGATGACACTCG11.3513513513513513No Hit
GACCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAG11.3513513513513513No Hit
AACAACTCTTGCCTCTCAGTCCCTCACAAGACAGCTGTCTCAGGCTTTTC11.3513513513513513No Hit
GGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCA11.3513513513513513No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA11.3513513513513513No Hit
GCTTCACCTGGACCCCCCAAAGACACACGTGACCCACCACCCCATCTCTG11.3513513513513513No Hit
CCTGGTGAAGGAGAATGCGGTGACAATGGAGAGAACCCCCAGGTTCAGTA11.3513513513513513No Hit
TCAGGAGAGTGTGCAACTGGCCAGCCAATTTCCCGGAGCCATCACCCTCA11.3513513513513513No Hit
CCCCCAGGCATTGAGGCCTGAAGGAGGAGGCCATCATGAATGAGCAGCTG11.3513513513513513No Hit
GGCACAATCTCAGCTCACTGCAAGCTCCGCCTCCCGGGTTCACGCCATTC11.3513513513513513No Hit
CCCAAGGTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAACCTCTGCCT11.3513513513513513No Hit
CGACTCGTCAATGTCATTTACAAAGGCTTCTTCTGCTGCCCTGTTGTTTG11.3513513513513513No Hit
CTCTTATCCATCTCCCTCCTCCCTTCCCTTGGGCAGGTGGATCCTGTGAA11.3513513513513513No Hit
GAGCAAGGTCGTCCTGGCCTATGAGCCTGTGTGGGCCATTGGTACTGGCA11.3513513513513513No Hit
GTGGACCACAGGCCAGCCAGTGAACCTCCTGGGCTTTCTTGCCTTTGTCC11.3513513513513513No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG11.3513513513513513No Hit
GCTTGGAGCGTCCTGCCCCCACCTGAAATGCCTTGCAACCCTGAAGACCC11.3513513513513513No Hit
ATGCGGCGCGCCTTGGCTTGCCGGGCCTTACGTCTGCGGATCTTCCGGGC11.3513513513513513No Hit
GTGGAAGAGCTCCTCCACGAACTCCAGCAGGCCGATGGCGATCTTGGCCC11.3513513513513513No Hit
AGAATGGTGTGAACCCAGGAGGCGGAGCTTGCAGTGAGCCAAGATCATGC11.3513513513513513No Hit
GAACTCCTTCAATTTCTCAGGCACAGTGATGCCCTTCTCTGTCTGGTAGT11.3513513513513513No Hit
GGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCT11.3513513513513513No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCAGGTACAGTGAAACTGCG11.3513513513513513No Hit
CAGGGCCCCACGGCGGAGGGGGCAGCCCAGGCAGGCCCGCACAGTCCTCC11.3513513513513513No Hit
CTGTCCTGCTCCGCCACACGGGCCGCCTCCCACTTGCGCTGGGTGATCTG11.3513513513513513No Hit
AGCTAGTTCTGTATTCATGAAGCTTAGGAACTCTGTCTTGGAGAGAGTGT11.3513513513513513No Hit
CTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTA11.3513513513513513No Hit
TCCTTCTCCAGCTGCTTCTTGCCATCCTCATCCTGCCATTTCTTGCAGTA11.3513513513513513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)