FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length146
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGGGCCGTAGGCAGCCATGGCGCCCAG34.054054054054054No Hit
GGTCCACACAGGTGCCGGCCACAGTGGCCAGGGAAGGTGCATCAGAGCCG34.054054054054054No Hit
ATTTTGGACAGTCCACGTTTGCTTCTTATCGTAGCCAGAGAAGAAGAAGC34.054054054054054No Hit
TCGCAGCTGAGAGCGCTCCCCGAGTTAGCCCATCTCGATGCCCTCAGGGA22.7027027027027026No Hit
TTTCTACACCGCCGTGTCCCGGCCCGGCCGCGGAGAGCCCCGCTTCATCT22.7027027027027026No Hit
TCCTCAGCCTTGCTAAACAGGGCCTTGCTCTGGATGGCTGCAATAGCCTC22.7027027027027026No Hit
CATCAGAGCCCTGGGCACTGTCGCTGCACGCAGCCTGAGAGTAGCTCCCT22.7027027027027026No Hit
GCATGTGTCAGCCCTCGCAGCAGAGCGCTCGCTTCTGGCTGTGGGAGGGC22.7027027027027026No Hit
TCAGTGGCTTGAGCTTTTCTTTCAGCTTCTTGCCTTCATTCACTGGAAGG22.7027027027027026No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA22.7027027027027026Clontech Universal Primer Mix Long (96% over 25bp)
GCACTGAGCAGCCCTGCTGAGGTCTCCCCAGCTACTGTGCCACAACCAGC22.7027027027027026No Hit
GCTCACCGGTGTGAATAGTAGAACAAGACTTCCTCGATCAGCGTGAAGAT11.3513513513513513No Hit
CCCCTTGGCCTCCCAAAGTGCTGGGATTACAAGCGTGAGCCACCACGCCC11.3513513513513513No Hit
GCACGTGGTATGTCCAGGCCACCTGTGCCACCCAAGGCACAGGTCTGTAC11.3513513513513513No Hit
CACATCCTCTGATTTATTCTCCCCAAAGACCACTAGATGCCGCTGTTTAC11.3513513513513513No Hit
GAGGTACATTGGTGATCGGAGTATAGTTGGAGCGCTTTGTCATGATTTCC11.3513513513513513No Hit
GGTGGGGCCAGAGTGAGCCCCTTCCTGCCACAGTCACCCCAACTGAAATT11.3513513513513513No Hit
GATCACGAGGTCAGGAGATCGAGACCATCCCGGCTAAAACAGTGAAACCC11.3513513513513513No Hit
GCGAGAAGTCCACCGAGTCCTGCAGGAGCCGCACCCCGGGCACGCTGCTC11.3513513513513513No Hit
GTGGAGGACTGCTTGAGCCCAGGAGTTTGAGACCAGCCTGGACAACATGG11.3513513513513513No Hit
GATGGTGATAACCCACTGCTGTCGCCACGATGCTCCATCTTCAGCCAAAG11.3513513513513513No Hit
CCACAAGCTCGGTGTCCAGGGTCTGGTCCTCCCCATCCCGCTGCCAGGTC11.3513513513513513No Hit
TCAACGCAGAGTACATGGGCAGGGCCGTAGGCAGCCATGGCGCCCAGCCG11.3513513513513513No Hit
GTTTTGCTCCTTGGATTCTGAGCCCGGGCATCGCACCTCGTTTATGAACT11.3513513513513513No Hit
CTCTGTGGGCTGAGGTGGGAGAATCACTTGCACCCAGGAGGCAGAGGTTG11.3513513513513513No Hit
CAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTG11.3513513513513513No Hit
GCTGCACATGGCATGTGTATCTCTGCTCTTCTCCAGAAGGCACCACCACA11.3513513513513513No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG11.3513513513513513No Hit
ACGCAGAGTACATGGGCATATCACCTTTTCTAACCTTCACAGCATAGATG11.3513513513513513No Hit
CCAACACGGAGAAACCCTGTCTCTACTAAAAATACAAAATTAGCCAGGTG11.3513513513513513No Hit
GCCCTGGAGAACCAGAAGAAGGCGAGGAAGAAGAAAGTCTTGATTGCCAT11.3513513513513513No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11.3513513513513513No Hit
GACAAGTGCAGGGGGAACTCCTCCTATAAAACCATCAGATCGGTTGGGCG11.3513513513513513No Hit
CCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGATGTGTAGGA11.3513513513513513No Hit
ACACCCAGTTCGTGAGGTTCGACAGCGACGCCGCGAGTCCGAGGATGGCG11.3513513513513513No Hit
CCATGAATCACACTGTCCAAACCTTCTTCTCTCCTGTCAACAGTGGCCAG11.3513513513513513No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11.3513513513513513No Hit
CACCCAGTTCGTGAGGTTCGACAGCGACGCCGCGAGTCCGAGAGAGGAGC11.3513513513513513No Hit
GAAGAGAAGTAAGCCGAGGGCGTCTTTGATTGTGTAGTAAGGGTGGAAGG11.3513513513513513No Hit
ACCCGGGGCACCTCCATCAAGGAATTCCGGGAGATGACCCTCAGCTTCCT11.3513513513513513No Hit
GATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCT11.3513513513513513No Hit
GCCAAGGAGAAGCCACCTTGGTGACGTTTAGTTCCAACCATTATAGTAAG11.3513513513513513No Hit
GTGCTGGAGACCTTCGTTGCCTGCATCATCTTCGCGTTCATCAGCGACCC11.3513513513513513No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA11.3513513513513513No Hit
GGCCACATATGCCCCTGTCATCTCTGCTGAGAAAGCCTACCATGAACAGC11.3513513513513513No Hit
CTCCGGTACCTGGTCCTCCACCTAGCCTCCCTGCAGCTGGGACTGCTGTT11.3513513513513513No Hit
CTTCCTCCTACACATCACAGCAGCGACCACAGCTCCGATGACCACAACTG11.3513513513513513No Hit
TGTCAGACCAGTCTGAGTTGGCAACGGCTGCCTGAGCTTGAGTATCTCCA11.3513513513513513No Hit
GCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTTG11.3513513513513513No Hit
GTAATGAATGGTGAATACTACCAGGAAAGTAATGGTCCAACAGACAGTTA11.3513513513513513No Hit
CCCCTACTGTGTTCAGACGTCGTTCTACTAAAGGCCCGTGCCGCCGGAAG11.3513513513513513No Hit
CGCCTGTAATCCCAGCACTTTGGGAGACCAAGGCGGGCGGATCACGAGGT11.3513513513513513No Hit
GGCGTGGGTTGAGGAGAGGCTCCAGACCCGCACGCCGCGCGCACAGAGCT11.3513513513513513No Hit
GGACTACAGGCATGCACACCACCACATCTGGCTTATGTTTTGTATTTTTA11.3513513513513513No Hit
CCTCACAGCCTTCCTGGGATACAAGGCTGGCATGACTCACATCGTGCGGG11.3513513513513513No Hit
GCCTTGTGCTCCCCTTTTCTTCAGTACAGGGCAGGGCCCTTATAATTTGG11.3513513513513513No Hit
GCCTCGTCCCGGGCCTGGGCCCCCCTGCCAGCCACTGTCTGCCTTGCTGT11.3513513513513513No Hit
CGCCCAGGCTGGAGTGCAATGGCGCGATCTCGGCTCACTGCAACCTCTGC11.3513513513513513No Hit
GACTTTTTTGATTTGTGAGACTTTCAGGTTAGCTAAAGCGTCTGCAGTAA11.3513513513513513No Hit
GTACGGCCAATCCAGTGCGTGCACCCGCTCCTTTATTAGGCTATAGAGCC11.3513513513513513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)