FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCACCTAGTGCATAAGTCAGACACAGAAAGATCACCTACTGCATAAG37.5No Hit
GGGTCAGGAGATCGAGACCATCCTGGCTAGCACGGTGAAACCCTGTCTCT37.5No Hit
GTCGTCTACCAGCTCGATAATTGGCGCAAGTTGTAGGTCGGGGGCACCAC37.5No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA37.5No Hit
CTCCTGACCTTAGAGTAAGTCTAAACTGCAAAGACCTTGCCTTTTGTCTG25.0No Hit
GGCTGTGGTCGACCGAGCCATCGGGGTTGGTCGCGTACTGCGCGCTGCTG25.0No Hit
CTGCAGTGAACAAGACAGAGTCCACCCTGGAAAGTGGCCACAGGGGAAGG25.0No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC25.0No Hit
GCATTCTCCAAAGGGTACGAAATGACACTGCCTGGGGGTAGAGCCAGTTT25.0No Hit
GGGCAGCCCTCGGCGGGCTCGGATTCTCTGGGAGATTTGATAGAGCTCCA25.0No Hit
CACTCATACCTGCTTCAGGGCCTTGGAGCATGCTGGTTCATATGCCAGGA12.5No Hit
GAAGTGACAAGTGCTCATACTGCAGAAGAAACCACAGGCAACAATCACAG12.5No Hit
CTTCATTGTGATGAGCGTGTTCTGGGGCTTCGTCGGCTTCTTGGTGCCTT12.5No Hit
CCGTAGCCCTTGCTGTAGATGGCAGCCGAGACGCCCACGATGAGCAGGGG12.5No Hit
TCTTAGAGCAGGGAGGGGGCTTTCTCAGTGAAATGTTTGGTTTTCTGCTG12.5No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC12.5No Hit
GGTTGGTTTGGAAGTCACAGTCATCCAGACGCAGGTGCTGGTTTTTCTTG12.5No Hit
TGCCCACCCCGCATGAGGCTGAGAAGCAGATCACAGGGCCAGAGGAGGCG12.5No Hit
CCTTCTTCCTTTGCAATTCGGTCTTTCTTCAGTGGTCCCATGAATGCTTT12.5No Hit
TCCTTGTCCTTCCTCAGGTCTGTCTTGCAGCCGATGAGCACCATGGGGAT12.5No Hit
GTACATGGGGCGTCGTCTCCCGGCAGTGCAGCTGCCGCTACCGCCGCCCT12.5No Hit
TAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGCTC12.5No Hit
CATCTGGTGCGATGGCTGCAAGAACCACATCGGCATGGGTGTTCGTTACA12.5No Hit
AATCCAGCCTGGGCAACAGAGTGAGGCCCTGTCTCAAAAAAACAAAAACA12.5No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA12.5No Hit
GCTCTGAGTGCCTCCCTCGTGTATCCACAGCATACAGAGTGAACTTGTAG12.5No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)