FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATGCTCCTGGGCAACCCAGGTCTGGTCTTCTCTCGTCTGAGCCCTGA410.526315789473683No Hit
GCACTGGGTCTTGCCCAAGGTCCACATGGTGACCACTTGCCTGTCTACCA25.263157894736842No Hit
ACTCAGCTCTGCACAGTGGTTACTGGGGGGCCTCTGGTACATTCAGGAGA25.263157894736842No Hit
CCTGGGGACCCTGCGCGGCTACTACAACCAGAGCGAGGCCGGTTCTCACA25.263157894736842No Hit
CTTCCAGAACACTCAGGGCCTCATCCTTGTGGTGGACAGTAATGACCGGG25.263157894736842No Hit
TTTAGGAACACAGATTGGGAGCAGGTACAGGAGAATCCTGGGTGAGGATG25.263157894736842No Hit
GTACATGGGTCTCGCGCCCTTGCCGCTGCCACCGCACCCCGCCATGGAGC12.631578947368421No Hit
GGCTTGATGTGGGGAGGGGTGTTTAAGGGGTTGGCTAGGGTATAATTGTC12.631578947368421No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT12.631578947368421No Hit
TCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTA12.631578947368421No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG12.631578947368421No Hit
GATGTAGCCTGATATGATGTCCCCATGCCCTGTTGGCCAGAGGCCCGAGT12.631578947368421No Hit
CCTCAGGATGGAAAAGATGCTGGAGGCTTTGCTGGCTTTCAGGATGCCGG12.631578947368421No Hit
TCTTTGCAGGCAAGCAGCTGGAAGATGGCCGTACTCTTTCTGACTACAAC12.631578947368421No Hit
ACCCCATTCTATGTAGTCAGCACCAGTGAATGGTGGGTTTGGCATTCAAA12.631578947368421No Hit
GTCTCGCTCTGTTGTCCAGGCTGGAGTGCAGTGGCGCGATCCCGGCCCAC12.631578947368421No Hit
CCCACGACCACGGCATGGTCTCTTTCATATGGCTCAAGGGTCAACTGCTC12.631578947368421No Hit
GTGAGAACCCCTTCAGTCCAAGCATACTGGTCCTTTCCAAGCTCACTGTT12.631578947368421No Hit
GAGTACATGGGACTGAAGATCCAAGTGCGAAAGGCAGCTATAACCAGCTA12.631578947368421No Hit
ATCCATAAGCATGGAATGTTTCTCCATTTGTTTGTGTCCTCTCTGATTTC12.631578947368421No Hit
CAGTTAGACTTGGAAAGTCATGCTTTTTATTTTACACACACATGCATACA12.631578947368421No Hit
TCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAGACGAATTGCCAG12.631578947368421No Hit
GGATAAAGCAACATTTATGGTTGGCAGCTATGGACCTCGGCCTGAGGAGT12.631578947368421No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC12.631578947368421No Hit
GGAGGACTCAGAACCAAGGATTTCCAAGTGATTTCTTCCAAAGCACAGGA12.631578947368421No Hit
ACATGGATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC12.631578947368421No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC12.631578947368421No Hit
GCATGGGAACAGGGGTCACGGTGGACACGGGGGTGGGCTGTCTCTCCACC12.631578947368421No Hit
GCCCCTCAGAGTTCTCTGTAGGAGCTGTAGACTGGGAATAGAGGCAAGGC12.631578947368421No Hit
CTCCTGCAGCGTGTTTTGTTCCACAGCCTCCACGAAGAGCTCATAGTCCC12.631578947368421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)