FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCGCCACCGCGCACGACAGCGGGTTGGTGAAGTAGGTGTGGCCGTGCA315.0No Hit
TTATGAACAAAGGCAGAGGCTGACCATGGCATGATGCATCTCAAAGAAGA315.0No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA210.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGGGCTCCATCCCGCCACCGTCGGTGCCGCAGACGCAGCAGCAGGCCCC210.0No Hit
GGGCGCCGGTGCGATGGCCGAAGATGCCGAGCTTGCCCCGCTTCACGCTC15.0No Hit
AGCACGCTTTCGGCGCCGACCGCGCGCACCTCGACGACCATGCGGCCATC15.0No Hit
CCTTCGTACTTGGCATGGCGCAGCTCCTCGACGCGGCCTTGTTGCTGGGC15.0No Hit
GTCCTAATTTTCTAGGCCATCTTTATGAAGTCTCCAGCTGTCAGTCCACA15.0No Hit
GGCGATCTTCGCATCGTCCGAATCTGGTGCACACAACCGGAGATCGTCGG15.0No Hit
GAATGGAATGGAATGGAATGGAACGGTATGGAATTGAATGGAATGGAATG15.0No Hit
AGACTACAGGTGTGCACCACCACGCCTGACTAATTTTGTATTTTTCGTAG15.0No Hit
CCGTCTTGGCTCACTGCAACCTCTGTCTCCCGGGTTCACGTGATTCTCCT15.0No Hit
AACTAGTACTTTGAAAGAAATAAGAAATTCAGAGTGAGTAGGTATATGAA15.0No Hit
TGTTTAGAAAGTAATTTTTAAAAGATTACACTGATAACCCCAAGCCAGTT15.0No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)