FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10
Sequences flagged as poor quality0
Sequence length146
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAACAGTCTGACCATGCGTGATCTCTTCGAAGCGCCGACCGTCGCGG220.0No Hit
GGGAAAGGACCGTAGGGATAGATGGTCGCGTGCGAGGCGGCGCTGCGCGG220.0No Hit
CGCGAAGGCCACGGCGCGGCCGATCGCATAGGCGGTCGCGTCGCCCTGCA110.0No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCCCCTG110.0No Hit
ATATAATTTTTCTACTAGGCTGTCAACAACTTAGGTAGGGTGTGTGTCTT110.0No Hit
CGCCCAGCTAATTTTTATATTTTTAGAAAAGATGGTGTTTCACCATGTTG110.0No Hit
CACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAA110.0No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT110.0Clontech SMART CDS Primer II A (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)