FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT218.181818181818183Clontech Universal Primer Mix Long (96% over 26bp)
TCTCCGGATGACGCATTAGCAGAAATCAAAGCGAAAGCTGTCGCTGCAAA218.181818181818183No Hit
GCCCACCTCGACCTCCCAAAATGCTAGGATTACAAGCGTGAGCCATTGTG218.181818181818183No Hit
CCACCGAGATCTACACTTTTTTTTTCGTCGGCAGCGTCAGATCTGTCTCT19.090909090909092Illumina Single End PCR Primer 1 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19.090909090909092Clontech SMART CDS Primer II A (100% over 21bp)
CACACACACACACACACACACACACACACACACGATTAGAATTGTTGCCT19.090909090909092No Hit
AATCATGGAAGCGGGCCTCCAAGTTCTAGCAGCTGTTGCGGACAAGTTTG19.090909090909092No Hit
ATACAAAAACTTGCCTGGCATGGCGGTGCGTGCCTGTAGTCCCAGCTGCT19.090909090909092No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)