FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT218.181818181818183Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCG218.181818181818183No Hit
AGAAACTTCTTTTGCAAGGTTCTGATGACATTGATTCCTGACAGTTAATT218.181818181818183No Hit
ATCTGACGCTGCCGACGAAAAAAAAAGTGTAGTTCTCGGTGGCGGTCTCT19.090909090909092No Hit
GGTGCAGGACCTCCTGTATGTCTGGCCAAAGCTCATAATCTAGTATACAT19.090909090909092No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA19.090909090909092Clontech Universal Primer Mix Long (95% over 22bp)
GCTGCTAGGAGAATAGCATCCGCCTCCTTGGCCGCTTCTAGAGTTGACTG19.090909090909092No Hit
GCTTTCAAGACCCAAGACCAAGAATAAGGGTGTTTTCATTTTTTATTTGT19.090909090909092No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)