FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length146
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTGTAGGATGCCTTTTAATTTTCTTTATTGTTTCCTTTGCTGTGCAGA39.67741935483871No Hit
TTAAAATACAGATTGGTTTCTTGTTTTCTGATGGCGGGTCACAGTCCAGT39.67741935483871No Hit
CTGCTGGCCTCATGGCTGGTCGCGGTCAGCCTGATCCCGATGCTGTCCGC39.67741935483871No Hit
GCCCGCCGGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGG26.451612903225806No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA26.451612903225806Clontech Universal Primer Mix Long (95% over 21bp)
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT26.451612903225806No Hit
CCACATAATTGGAAGTAAAGCACTAGTCAGCAAATGTAAAAGAACAGAAA13.225806451612903No Hit
GTGTTCGCTGGGTCGGGGACGACGGCAGCCGTCGCGCAGAAGATGGGGCG13.225806451612903No Hit
GTTTTAGATAATCCGGCTTACCATCACGCTCATCGAGCCGCACGAAAAGA13.225806451612903No Hit
CCACTCACAATCGCAAAGACGAGAAATCAACCTTAATTCCCATAAATGAC13.225806451612903No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13.225806451612903Clontech Universal Primer Mix Long (96% over 26bp)
GAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAA13.225806451612903No Hit
ATTTTATGGAGGCAATAATGAAAAACAGGAATTTTAACCTGCTGAATATT13.225806451612903No Hit
GCATAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG13.225806451612903Clontech Universal Primer Mix Long (96% over 26bp)
TTTCTGAATATACTGAGATCCATGAATCAATACATTTACGTCTTTTTACC13.225806451612903No Hit
GCCTTTGAGTCAAGACTATGGGGTTTTCTAGATATGATTATGTTGTCTGC13.225806451612903No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA13.225806451612903No Hit
GTGTGCAGGAAGGACTTGCAGAAGGTGTGTGCCAGGTCTGTCTCTTATAC13.225806451612903No Hit
GAAGGAAAAAGGGAGGATTCATATGGCTTGGCTGTGTCCCCACCCAAATC13.225806451612903No Hit
GGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGA13.225806451612903No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGC13.225806451612903No Hit
GACTTTATGAATTCAAGATGTCATTTGATCTAATTCTAAGACTTCAGGTA13.225806451612903No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)