FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length146
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTACCAAAACTTTCATGGAGTAAGCAACAAAGTGAAAAACAGACACTTG39.67741935483871No Hit
ACGTGCAGGCGCTCGCGGTTCTCGTCGATCCAGTTCTCCACCCGTGCCAC39.67741935483871No Hit
CTGGATAGTCACATGCAAAAGAATAAAATTTGGAACCTTATGATATACCA39.67741935483871No Hit
ATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCA26.451612903225806No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26.451612903225806Clontech SMART CDS Primer II A (100% over 21bp)
GGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC26.451612903225806No Hit
ACCTGGCACACACCTTCTGCAAGTCCTTCCTGCACACCTGTCTCTTATAC13.225806451612903No Hit
GTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAA13.225806451612903No Hit
CAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATG13.225806451612903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACC13.225806451612903No Hit
CCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAAC13.225806451612903No Hit
GCTACATAAATCTACATAGCTAGAAATACCATGGTACTGAGTGGAGGAAA13.225806451612903No Hit
GCGTTGTGCGCTCGGACATGCGTTCGACGAGGNTTTGTTCCGCAAGGCCT13.225806451612903No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13.225806451612903Clontech Universal Primer Mix Long (96% over 26bp)
ACTAATATCATACTGAATGGGCAAAAGCTGGAAATATTCCCCTCGAAAAC13.225806451612903No Hit
CCCTTAGTCCGAGTGATCCCGCCCGGGTCTTGATCGTTGAGCACCTTCTC13.225806451612903No Hit
TCCATGGGCTCAGTGGTGATATCCCCTTTATCATTTTTATTGCATCTATT13.225806451612903No Hit
ACTTACAATCATGGTGGAAGGGGAAGCAAACATATTTTTCTTCACGTGGC13.225806451612903No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT13.225806451612903Clontech Universal Primer Mix Long (100% over 23bp)
AGAACAATTAAATTGCCTACAGATTTGTCATCAGAAACCATCAAAGCCAG13.225806451612903No Hit
CTCCCTTCTCTTGTCTCCTCTGCTCCCGCTTATCCACCCTCCTTTTCTCC13.225806451612903No Hit
GGGGTACATGTGCAGGTTTGTTATATAGGTAAGCTCAAGTCAAGGGGGTT13.225806451612903No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)