FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length146
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCACATTAGCATTTATGCCGACGTGATTGAAAAGTCGCTCGGCATCC311.11111111111111No Hit
GTGTGATTCTTTCGCCGCCGATGTTGAGGTGCTCGCTCTTACGCGGGCAC27.4074074074074066No Hit
GCCCTTTAATTACCCTCTTTCAAACCAATCCTTTTCGAGCATCTGATGGT27.4074074074074066No Hit
GCACTGAAGCTGGCCCGCAAGGTCACCGGACGTTCGGCAGTCATCAACTT27.4074074074074066No Hit
CGTATACATAGACACACACACAAATATAAGAGACAATCGGAAATGTAAAC27.4074074074074066No Hit
AGATTAGGCTTAGACTGTGGGCTGATGCAGATTCAAGTGAAGCAATTCAT27.4074074074074066No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT13.7037037037037033No Hit
AGCCCAGGTTGTAACTTTCTGTAAAACTTCAGTTTACTTACCGGTACAGG13.7037037037037033No Hit
ACCTTGAACATTCTTGTACGCACTTTGATGTACATGTTTATCGTTTGCTC13.7037037037037033No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAG13.7037037037037033Clontech Universal Primer Mix Long (95% over 21bp)
TGATACCATTCAATTACATTTGATGATGATCCCATTCAATTTCATTTGAT13.7037037037037033No Hit
ATTTGGCTCTCTGTTTGTCTGTTGTTGGTGTTTAGGAATGCTTGTGATTT13.7037037037037033No Hit
ATGGTGGCCCGCCAAGGAGTGACCCGTCAAACGGTCCGCAAGGATGCCAG13.7037037037037033No Hit
CCCCAGCAATGGCATGTCTCTCCTTTAAATTCCCATCTGTCTCTTATACA13.7037037037037033No Hit
GATCAAGTCCAAGGCCATTGGCTATCTCAACACTGGTTACCAGAGACAGT13.7037037037037033No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGAGGTA13.7037037037037033Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAGAGGCTGTAGATGATTCATATTTCCAATTGGGAGGGAGGACTCGC13.7037037037037033No Hit
TGCCAGAGGTTGCCACCCCGGAGTCTGAGGAGGCCTTCACCCTGCCTCTC13.7037037037037033No Hit
ATCTCATCTTGAATTGTAGCTTGCATAATTCCCACATATTGCGAAAGGGA13.7037037037037033No Hit
GGCAACACGTAGTGAAGTCTTGTTTCTTTATAAGTTCAGCCAATCTCTAT13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)