FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length146
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTTGAGCGCACCGCACAAGCTCACGCCCGAGACATCCTCAATAGCGC311.11111111111111No Hit
CACTGCCGCGGGGCGGTTGATTCCACTGCCGGAATCATCTAGAACGCGGG27.4074074074074066No Hit
GGGATAGGTATGCATGAATATGGACCTAGGCACACAGTTAATAGTGGTTA27.4074074074074066No Hit
AGATGAATTGCTTCACTTGAATCTGCATCAGCCCACAGTCTAAGCCTAAT27.4074074074074066No Hit
ATCTTCCAGTTTCACCAGTTATCAGCATATAGCCAATCTCTTATCCATAC27.4074074074074066No Hit
TTAATGATGATGTCGACGTCGTCAAGCAGAGGCGTTGCCTCCACAGCAGC27.4074074074074066No Hit
AGTACATCCAATGATTCCATTCGAGTCCATTAGATGATTCCATTTGATTC13.7037037037037033No Hit
CCATGCTGCTGATGCTCTTGACAGGTTCCACTGAGGGCTTGGGCTCAGGG13.7037037037037033No Hit
AGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACG13.7037037037037033No Hit
ATATGAGGGCTTGGGTAATGTGTGCTTCATCGATGAAGATGTAGTCTCGA13.7037037037037033No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACACTGCGTTTAT13.7037037037037033Clontech Universal Primer Mix Long (96% over 26bp)
CCTTCACAGATCGGTGTAATCCTGAGAGATTCACATGGTGTTGCACAAGT13.7037037037037033No Hit
GCTTCATCCATGTCCCTACAAAGGACATGAACTCATCCTTTTTTATGTCT13.7037037037037033No Hit
GATTTACTATTAAAATATTTGATTTGTTTCCATTTCTCCAGATCAGACCC13.7037037037037033No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTGCGTTTATAACACTGGTTCCCATG13.7037037037037033No Hit
GGTTTAATGGACTCACAGTTCCATGTGCTGAGGAGGCCTCACAATCATGC13.7037037037037033No Hit
GTACAAAAATCACAAGCATTCCTAAACACCAACAACAGACAAACAGAGAG13.7037037037037033No Hit
ATGTCACCCTCCTCATCTAGAGGGTCGCCGGTCTCCTCGTCATCGTTTTC13.7037037037037033No Hit
ATGGGAATTTAAAGGAGAGACATGCCATTGCTGGGGCTGTCTCTTATACA13.7037037037037033No Hit
GAATTCATCACCACTACATTGGCTCCATGAGAAATGATTAAGGGAGTCTT13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)