FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length146
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG23.4482758620689653No Hit
TCAGCCGGGTGCCGGAGAGATTCACCAGCAGCGTGAAGAACAGGTAGAGC23.4482758620689653No Hit
CGGCCGCGATCCGGGTGCCGGCGACCGTGGTCGCGGTCGAGGGCGACCGG23.4482758620689653No Hit
CCCTGGCGAAGATGGTCGACGGGGTGTGGCCGGCCTACCAGGACGTCTAC23.4482758620689653No Hit
AGTCGGCGGTGTGGCCGGGCAGCGCCAGCCGCGTGAGCCGCTGGCTGCCG23.4482758620689653No Hit
GTGCAATAGCACGATCTCAGCTGACTACAACCTCCGCCTCCCGGGTTCAA23.4482758620689653No Hit
GATGCAATGTAAAGACCCACCTGCCATTGCTTGCTTTGAAGATGGAAAAA23.4482758620689653No Hit
GGATCGACATCAGCGTCGACTTTCCGGCGCCGTTCTCGCCGAGCAGCGCG23.4482758620689653No Hit
GAATCCCTCCGGATCGCAGGCGGGATCGGGCGCGGGCCGCATCATCATCG23.4482758620689653No Hit
GAATGGAATGGAATGGAATGGAATAGAATGGAATTGTATGGAATGCAATG23.4482758620689653No Hit
ATCTGGCGCAGGCGCTGGGTGTGGCGGCGGGTGATGTCCATGTCGGCGAA23.4482758620689653No Hit
GTCTCACCTTCTGGGACCTCGATCGCGAATTCGTGACAGGCGGCTTCGGC23.4482758620689653No Hit
TTCATATTCTTCCATCTACCAATTTGATATCAGTTCTTATTTTTGTATGT23.4482758620689653No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.7241379310344827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTGGCGCTCTGCCAGTAGTGTATCCACCATTGAGCGCACCCCCAAAT11.7241379310344827No Hit
CCGCTGGGCCATCCGACCGCCGACAGACCCGACCGATAGCCTTCGCGCCT11.7241379310344827No Hit
CAGTCAATTCATTCGATGACGATTCCATTCAATTCCGTTCAATGATTCCA11.7241379310344827No Hit
TCCATTTTGCTTCTGTGGTAGTAAGGATTGGGAAGAGGGCAGAGATGGGA11.7241379310344827No Hit
CGCCACCGCCGCGCTGCCGCCGGAAATGAACTGGCGCAACGAGCTTTTCG11.7241379310344827No Hit
CGCTGGACGCTGTGGCACCAGCTGGCGCGCACGCTGCGCGCGGTGTCGCA11.7241379310344827No Hit
GGTTAAAGAAGATCGTTTTACCACCATTCATATTCAAGAATTAACCTGTG11.7241379310344827No Hit
CAAGGGACCTGTCCGACCCGACGCGCCCGGACGCTTCCGGAGTGTTCACA11.7241379310344827No Hit
GTTCTCATCTTGAAACTAGTGATGGCTGACACAGACATATTTCTAGGACC11.7241379310344827No Hit
GCATCCAGGAATGAATCCACTCATTTACAGTCAATTGATTTTCAACAAAC11.7241379310344827No Hit
CCCATGACCACGCCGGCGGAGGGCGCCTCGTAGACGTCCTTCTCGATCTT11.7241379310344827No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGTTTAA11.7241379310344827No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.7241379310344827No Hit
CACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCAT11.7241379310344827No Hit
ACTAAAGTCTAAACAGAAACCTTTGGAAATTGTAGTCCTAAGAATGTTCC11.7241379310344827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTGGGTCT11.7241379310344827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTATGGGTAT11.7241379310344827No Hit
GCGTTCCCTGGGCGACGAACTGCGCCCCGTCGGTGAAGCGATGCGCCCGC11.7241379310344827No Hit
GGTTGTAGGCCAGGCGGATGGTGAGCTGGCCGTCGTCGGCAAGCTTGCGG11.7241379310344827No Hit
GAAACAGCGAGACTCCATCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.7241379310344827No Hit
CTCCATATCACAGCAGCAACCACAGCTCCAGAGACCACAGATCCAAGGAG11.7241379310344827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGT11.7241379310344827No Hit
CTCCCAGCTTGTCCGCAAGCTCGGCAAGGGGACCGGCAAGTACGCGAACC11.7241379310344827No Hit
GCTCTGGATCGTCGCCGGGCTCGTCCTGCAGCTCCTGCTGCTGAAACCCG11.7241379310344827No Hit
CCCAGAAGCGGTCGGGCAGCTTGCCACCCCAAAGGCGCCAATCCGCGGAC11.7241379310344827No Hit
CATGTACTCTGCGTTGCTCCCACTGCTTCCCATGTCCTCTGCGTTGCTCC11.7241379310344827No Hit
CGTCCGGTGCGGGTACTGCGACTTCAACACCTACACCGCCGGCGAACTGC11.7241379310344827No Hit
GATGATATGTATACAGTTTTGAAGCAAAAGCCTCATCCACGGAAAGATGA11.7241379310344827No Hit
CACTTCGCTCCGACTTTCATCCGGCTGCTTCCGTGGTGCTTCCGCGGAAA11.7241379310344827No Hit
TTGACGGGGCGGCGTTCGTCGCGCCGCGTAGTGACTGCTTGCCGTTCGTC11.7241379310344827No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTT11.7241379310344827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)