FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length146
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTGTCGAGCACGCGGAGCTGCTCGTCGTGGCTCGGCTTCTGGTACTT23.4482758620689653No Hit
ATCGCGGTCGATGCCGGCGCGGACGGCGCGGGTGGCGCGCTGGCACTCCG23.4482758620689653No Hit
GCCAGCCGCCGCTGCGTGCTGGTCGACCCGCTGCCCGAACTCACCGGCCA23.4482758620689653No Hit
GTGCTTTTGCGGGTAGCGGCCCTGCAGCAGCAGCGCGGTGTAGCCGACGT23.4482758620689653No Hit
GATCTCCACCATCGTGCTCTACCTGTTCTTCACGCTGCTGGTGAATCTCT23.4482758620689653No Hit
GACAACATTTTCTCCTCCTAAAGGAAGCAGGGTAAATGTTTTTATATTCT23.4482758620689653No Hit
GTCGATGGGCGGCATGATCGCGCAGATCCTGGCGAGCTACCACCCCGACC23.4482758620689653No Hit
GCTTAATAGGAATCAACAAATATACTTGGGGTGGGTTGTAGAAAAAAGGG11.7241379310344827No Hit
GTACCTGCCAACATGAAACAGGAAGAGGGGTCTATTTCATTGCTGTTTAG11.7241379310344827No Hit
ACCCCTTCGTCTGGGGAGTCTAGCGGGCCTACCGCCGTCGGCGTCGACGA11.7241379310344827No Hit
ACGCAGAAAATAAAAAAAAAAAAAAAAAAAATTTAAGATCGTTTTAAACT11.7241379310344827No Hit
AGGTACTGCAGGAAGGTCGCCTCGAGCCCCGCCTGCGAGGCTTCGTCGAT11.7241379310344827No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GGCAGATAGATATTCTTTTACCTCAGTCACCTTTTAATTTTTGCTTTCTC11.7241379310344827No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAAGTTGCGCGCCTGCTGCCTTCCTT11.7241379310344827No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
CTTTTGGGATCATATCCAAAAAATTATAGCCCAGACCAATGCCATGGAGC11.7241379310344827No Hit
CCACAGCATCCGGAGTGAGACCGAACGCGTCGCGCACGCCCGCCAGCATC11.7241379310344827No Hit
GGCAGATAGATATTCTATTACCTCAGTCACCTTTTAATTTTTGCTTTCTC11.7241379310344827No Hit
CAGTGGTATCAACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAG11.7241379310344827No Hit
TCCGTGAGCTCTCCGAGATCGCGGGCGACAATCTGCGATTCGAGACCATC11.7241379310344827No Hit
GGGTAGGTCTGGTTTCCGCCGCCCGCGCGCTCAAACCCCGGGGCGTGACC11.7241379310344827No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT11.7241379310344827No Hit
ATCGAAGAGAATCATCAAATGGACGCGAATGGAATCATCTAATGGAATGG11.7241379310344827No Hit
TTTACCGGACGCGAAGATGCGAAGGCCGAAGCCGGGAAGTTCGTCGTCCC11.7241379310344827No Hit
GGTAAGGGCTTTTGTCTGTTTTATTCATTGCTATATTTAGTGAATCACTA11.7241379310344827No Hit
GGTCAGGGCTTTTGTCTGTTTTATTCATTGCTATATTTAGTGAATCACTA11.7241379310344827No Hit
CACCAAGATATCGCCACCTTGTACTTCCGCACCAATATAGACGATACCTG11.7241379310344827No Hit
CCCCCTTCGTCTGGGGAGGCGAGCGGGCCGCCCGCCGGCGGCGTCGACGA11.7241379310344827No Hit
ACTCCATTCCTTTACATTACATTCCACTCCATTCCATTGCATTCCACTCC11.7241379310344827No Hit
GGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCT11.7241379310344827No Hit
ACGCTGAGATGGGGTGGTGGTTGGGTTTGGGGGGGGGGGGGGGGGTGGGG11.7241379310344827No Hit
GTGCGGGCGCATCGCTTCACCGACGGGGCGCAGTTCGTCGCCCAGGGAAC11.7241379310344827No Hit
ACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATATGCTTTTAAATT11.7241379310344827No Hit
GGGGGGGGGGGGGGGGGGGGTGGGGGGTGGGGGGGGGGGGGGGGGGGGGG11.7241379310344827No Hit
GCCATAGTAGATGCCGGCGAACATGATGAGGCTGGCCGTGGGGTCGAACT11.7241379310344827No Hit
GCCAATCACCAACACAACGTGCCTGAGGTACAGCTTGCGATGGCGCATAC11.7241379310344827No Hit
GGCCTGCAGGGGATGGAACCTTCCAGAAGTGGGCAGCTGTGGTGGTGCCT11.7241379310344827No Hit
GCGCGAAGGCTATCGGTCGGGTCTGTCGGCGGTCGGATGGCCCAGCGGCT11.7241379310344827No Hit
GTGGTCAGTTGCTCTTCGGTCACCGGCGCCAGGCTGAGCGTGACGCGCAC11.7241379310344827No Hit
CGCCCGTACAATACAGCGTGCGCTGTCGGGCCTTCTCATCGTCCTGCTGG11.7241379310344827No Hit
CTCACTCAAACCTACTTCTACTCCCCCTTCTTCTGTAGGCCTCTTTTCAT11.7241379310344827No Hit
CCTCCACGCAACGACCGCCGATGCGCTGCGACGGATCGCCAAGAAGCACG11.7241379310344827No Hit
GCCAGGGACTGTTCTAAGATTATCTTAGAATACTCATTACCCTGTCAGAT11.7241379310344827No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAACATGGAGT11.7241379310344827No Hit
GCCACGCCTTCGGCGACCAGTACCGCGCCACCGACTTCCGCTTCCCCGGC11.7241379310344827No Hit
GACTAGCCAAGCGTATTTAAATTTGGTCGCACAGCGTGCCACGATTAAAG11.7241379310344827No Hit
AAGCGACGGCAGATCGGCGCAGAGATCGCGAAAGGCCTGGAGCGCCTCCT11.7241379310344827No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG11.7241379310344827No Hit
ACACCATTCCATTACATTACATTCCACTCCATTCCATAGCATACCACACC11.7241379310344827No Hit
CGCCCGTACCATCCAGCGTGCGCTGTCGGGCCTTCGCATCGTCCGGCTGG11.7241379310344827No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)