FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63
Sequences flagged as poor quality0
Sequence length146
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTGTAGGACAACAGCTCAGCCTCTATGCTGCCATTTTAGTTCTTTCT23.1746031746031744No Hit
GCCGTGGCGCGTGCGAACTTCAACAGCCGGGCCGTGACGGCGGATGCCAC23.1746031746031744No Hit
CGATCTGGGAGCCCGATCAGGTCCGGCAGGAACTGCGCCGGGCGCTCAAG23.1746031746031744No Hit
CATTTATATTAAGTCTAAAATTACTTCAAAATGAAAGATTAAAAATCATT23.1746031746031744No Hit
AAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGG23.1746031746031744No Hit
GAAGCCGTACACCACGGCGGTCGCGGCGTCGGCGGCGATCAGCAGCGGAT23.1746031746031744No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA23.1746031746031744No Hit
CGCCGGGTGAGTGGCGCCTTCGTCGGATGGGGTATTCCTACTGCCGAGGC23.1746031746031744No Hit
GAGCAGTTCGACGATGCCGGCATCAAAGCGGTTGCCCTCCACGGCGACCT23.1746031746031744No Hit
GCTTCAAGCCATGAGGCAGCGGGGCATTGCTGTGGAAGTGCAGGACACGC11.5873015873015872No Hit
GACCACGATGTCGCACTGCGCGCCGAGCTCGGCCAGCGTGGCGCAGGCCG11.5873015873015872No Hit
CTTCTCGACCGCGCCGGGCTCATCGCCCCGCGCCACGGCACGGCCACGGA11.5873015873015872No Hit
CTCCCATTCTGTGGTTGTCTTGTTTACTCTGACTGTTCCTTTTGCCATGC11.5873015873015872No Hit
GTCTCACTCTGTTGCCCAGGCAGGAATGCAGTGATATGATTACAGTTCAA11.5873015873015872No Hit
CTTCTGAGTGGGGGTGATGGCATGTCCAGTTTGCCTGGGAAAGTCCTGGC11.5873015873015872No Hit
CACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCAT11.5873015873015872No Hit
GCACTTCGGCAGGCGCGGTGGCTTCGGCAGGCACCCGCTCGGCGACGGGC11.5873015873015872No Hit
GGTTGGTGGTGACCGGCGGCTACCTGGCGTTCTTCCATGACACGCGAGCC11.5873015873015872No Hit
CCACAGGCGGCTACGGGCTGAGCGGCTACTTCTTCTACATCCCGTGGCTG11.5873015873015872No Hit
GCCCACGACTACACCCTCATCTTCGAAGAGCCACTTTGCCGACGAACCCC11.5873015873015872No Hit
GTGGTGTCTTTGACCGGGTCGGCGATGGCGAGGATGGCCGCCACGCGACC11.5873015873015872No Hit
CCTGAAGTCAGGAGTTTGAGACTAGCCTGGCCAACATGGTGAAACCCCAT11.5873015873015872No Hit
TCCACACCCTAAAGGATTGATAATGGTTCTCAATCATTGCTATGGAGAGG11.5873015873015872No Hit
GCTGAAGGGTGGCAAATGCGCTGTCTTCGCTCAAATGACGCAAAAAACAC11.5873015873015872No Hit
CACAGCGCCCGCACCGTGGCCCACCTCGAAAGCTACATCGGCCTGGTCGA11.5873015873015872No Hit
GTTATCTGTTTTCTGGTTGTTTTGTGGTCTTCTCTTCCTTTATTTCTTCC11.5873015873015872No Hit
GTGTGGTCCGGGGAGCAAGTATTCCTTTCCAATTCCGTCCAGAAAATGAG11.5873015873015872No Hit
ATCTTCGCGCTGCGCAAGGTCGCGCGCACGTACGGCGGCATCCAAGAGGT11.5873015873015872No Hit
CCCTCGCGCCCTGTGAAAGCCGATGGCAGCGGTGAATTCATTTTTTACCC11.5873015873015872No Hit
CACAATGCCTGGGAGCTGGGATAGGGCGCGCGCTCCACGTCCATCACCGT11.5873015873015872No Hit
GCGCGCGCTCTGGCCCTATCTCGATCGCTATCGTTTGCTGATGATCGGCG11.5873015873015872No Hit
CCCCTTCGACGCCGCGCTCGCCGCCCGCCATGAGATCGAGATAGGTTTGA11.5873015873015872No Hit
GTCGGGGGCCCAGCCGGCGCCCTCGAGGAAGGCGCGGCCCGCGTCGTCGG11.5873015873015872No Hit
CGGCTTTTCACCGAATCCGCCCATCAGCTGACACAAACCCACGTCAACGC11.5873015873015872No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAATTAATCCTGTC11.5873015873015872No Hit
TCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGT11.5873015873015872No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.5873015873015872Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAACACT11.5873015873015872No Hit
GTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCC11.5873015873015872No Hit
TGCGTGATCTCCATCGATCTCCTTGTCCTCGTGTGCCAGCGCCAGCCGCG11.5873015873015872No Hit
TTCTGAGAGTGGGACACAATTTCCAGGAATATCTGTCCCAGACTCTTAAA11.5873015873015872No Hit
AGGTCGGCGCGGATGGCGTGGAAGGGGGCGCCGGTCTCGGAGACAAGGGC11.5873015873015872No Hit
TTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACA11.5873015873015872No Hit
CCGCTCCCACCGATCCGGGGGACGCCGGTCTGGTCGGCGAGCCGGTGACC11.5873015873015872No Hit
CCCCAAACCGGGGTGACTGTTTTAGTGTGCGCGGTATCGCCCGTGAGCTA11.5873015873015872No Hit
CCCCAGTGGTGGCGGCAAGTCGACCCTGTTCCGTTGCCTTACCCGGTTGA11.5873015873015872No Hit
GATGGATTCGGGCCAGCTGGTCGAGCACGGCGCGCCGCGCGGGGGCGCTG11.5873015873015872No Hit
GCTAGGACTGACGCCGAAGCGAAGTGAGAAAGGCGCCGCAGTGCGGCGCC11.5873015873015872No Hit
GTCACGGCCGCCTGCACACCGGCGACCCGATCGCGATGGCCAAAGACGCC11.5873015873015872No Hit
CAATGGAATGGAATGAAATTGAATAGAATGGGATCGAATGGAATGGACTG11.5873015873015872No Hit
ACCTGCGCTACTGACGCGAGGGAATCGGGGGGCACCGTCGCAGGGCGGTG11.5873015873015872No Hit
GGAAAGAGCCTGTAATGGGACACTCCTTCCCGCTGAAGCTCCGAATCCCA11.5873015873015872No Hit
GTTGATTTTGTTTATGTTTTCAAAAAACTAAGCCTTTGTTTCATTGACCT11.5873015873015872No Hit
CATCTTCATGCAGACCAAGAACCTGAACGCCCCCATCGTCGAGGCCACGC11.5873015873015872No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)