FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63
Sequences flagged as poor quality0
Sequence length146
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGA23.1746031746031744No Hit
TCACAACGGTGCCGCTGCGCCCCGCACGACCCGTGCGACCCGAGCGGTGC23.1746031746031744No Hit
GCCGCATGTCAGCCACCCCGGCGGCGAACATGCTGACGCGAAGCTCCAGC23.1746031746031744No Hit
GGACACGAGGTCCACCCAGTCGAACTGCTCCCCGATCGGCAGGCCGTCGA23.1746031746031744No Hit
GTATATGTAGTTCCCAACACTGTGTTCTGCACATAATAGACAATAAATGC23.1746031746031744No Hit
TTCCACCTCCGGGTGGCTGCCGGCCCGGGGCTGCTGCAGGGCGCGGGAGA23.1746031746031744No Hit
ACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAAC23.1746031746031744No Hit
CCCGGGTGGTGGCGGGCCTGCACCCGGTCGAGATCCAGATGGCGATGATG23.1746031746031744No Hit
GAACTAGAATTTTATAGGTACCTCAATAAACCTGAAATTCCCCAAATCCA23.1746031746031744No Hit
ATCCAGGTTTGGTTGTTCAACACCTTCCACACCACACCTTCCGCTTCATC11.5873015873015872No Hit
GATTGGAGACAATTCCCTATGGCCTTTGTGTAGAGATCATTCCCGAAGGG11.5873015873015872No Hit
CTTAACCTAAATACATCAGCACCGTCTCCTAAAGACGGAATAGTGTTCTT11.5873015873015872No Hit
GATCCATTCGTGTCCATTCCATTCCAGTCCATTACATTCCCTTCAAATCC11.5873015873015872No Hit
CCTCCGGAAGGAAAGAGAAGCAGAGGTGTGGGCTGGAAGTGAGAAGGCAA11.5873015873015872No Hit
GGTCTGTATCGCCAGCGGCACTACGAGGTTGAGTTCGACGATCAGCCAGA11.5873015873015872No Hit
TCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAA11.5873015873015872No Hit
CCGCCGAGCACGAAGCTCGGGCGCACGAGCACCGGGTACCCGATCTCCTC11.5873015873015872No Hit
CCGCTATCCGGCTCGATCAACCGGGTAAGGCAACGGAACAGGGTCGACTT11.5873015873015872No Hit
CCCACGGAGATGTGTATAAGATACAGACCAGGCAACGACCTTACCAAAGG11.5873015873015872No Hit
CGTTCAGCCGTCCGGTGATCCAGAGGCCGCCGACAAGGAGCGCCCCGATG11.5873015873015872No Hit
CGCCGGACCAGGGTCCAGCCCGCGCCCAGCGTCTCCGGGTGGGCGGCCAG11.5873015873015872No Hit
CAACCAGCGCGTTCACAACGCCCTCGTCGGGGGCGCGGCGGCCGGGGGCC11.5873015873015872No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA11.5873015873015872Clontech Universal Primer Mix Long (95% over 21bp)
GTCGGCCGTTACGGGATGGAGGAAACGGAAGCCCTTGTCGCCGAGACCGG11.5873015873015872No Hit
CCTGTGCCGTCGCCTCGTAGTCGTGCGGATCCTGGGTGGGTTTGGTGATG11.5873015873015872No Hit
GATAAGTGCCAAAGGGTAGATAAGAGTGAAAGCATTGGGAAGCCTGGCAG11.5873015873015872No Hit
ACCCTTACGCGACCCGTCATCAACGGGTCGCGGCGCGTGTGGCTCGTGCT11.5873015873015872No Hit
GCAGACGCATACTGAACGGGGCGTGGATTTTGCCACCTGTTTTCAAGGGC11.5873015873015872No Hit
CCATCGGCACCGTGCAGGGCCGCCGCGTCGTGATCGGCAGCTCCTCCTTC11.5873015873015872No Hit
GTCGGGGGTCAGCGGCGAGACCCCGCCCCAGTCGTCGATGCCGGCGCGCA11.5873015873015872No Hit
GGCGGGCACCGGCCCGATCTTGGGTCCGGCTGCACGCGCTCCGGTCGACA11.5873015873015872No Hit
GGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCT11.5873015873015872No Hit
CTTTGCGAGAGATCAAAGCAGCCATGGCTACCACGCTTCCGCGTGAAAAG11.5873015873015872No Hit
CCACCACAGGGCCAAGGTCCAGCCCATCGGGCAGGTCGTTCTGATACAGG11.5873015873015872No Hit
TATTAGTTTTATTTTTTCTTGTTAGTTTGCTTATGCAAGCAGTGTTGTTA11.5873015873015872No Hit
GACAGTTGGAAGGCTGCTACCAGCGAACTTGTTATTTTGAATCGTGCAAC11.5873015873015872No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.5873015873015872Clontech Universal Primer Mix Long (96% over 26bp)
CGTCGCACCGGGCGCCCGGCCCTGCAACTGAAGGAAGCAACGCAATGAAT11.5873015873015872No Hit
CTAGAAGATGTGTCTTTGAGGGTGTCTTTCAAGTTGTCCTTTAGGTTACT11.5873015873015872No Hit
CATTAAACGGTAAGCGTAATATAAGATTAGCACTAAAAAGGAGCTACGAG11.5873015873015872No Hit
CCCGCCGCACCGCGAGCGGCAAAGCGAGCGACAGGCCCGCCGTCACCGTG11.5873015873015872No Hit
GCACTCCGGCCTGGGCAACAGAGTGAGACTCTGACTCAAACAAACAAACA11.5873015873015872No Hit
GCCCTGCGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTG11.5873015873015872No Hit
GCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGGCGC11.5873015873015872No Hit
CGTGGGGGGTGGGGGAGGGGCCCCCCCGCGGGGCGGCCGGGGGAAAGCGG11.5873015873015872No Hit
GCTCCCGCCTGCTCCAGGCGGCGGTCGAGACGCTCCAGGCCGACCGGTTC11.5873015873015872No Hit
GTGCAGGCGCTCGGCTGGGCTCCGGCGAAGCTGGGTTTTCCGGTGCCCGA11.5873015873015872No Hit
GTATCAAAATGATGTCAGATTTTTAAAGCAGTTTGCTTAGGGGAAAACAA11.5873015873015872No Hit
GCATAATAAGGACAGGCGTGGTGGGTCACGCCTGTCCTTATTATGCTGTC11.5873015873015872No Hit
ACTTCGAGGACATCGCGGCGGCCCAGGCCGATGCACCGACCACCGAGCCA11.5873015873015872No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATAACAATGGTGTA11.5873015873015872No Hit
TCGCCACGGTCTCGAAGGTCTTCTTCAGCTCGGCGAACACGTCACCACCC11.5873015873015872No Hit
GTTCAAGGCCGGCCTGACGGCCGGCAGCAGAGCGGATCGGTGCGGCGGGG11.5873015873015872No Hit
TCTGGCGCTCGGGCGTGGCCGAGACCAATCCGCTGATCATGGAGAAGCTC11.5873015873015872No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)