FastQCFastQC Report
Fri 10 May 2024
EGAF00003650900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Total Bases1.8 kbp
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACACCCGTTTTCCGCCAGCTACAGTATTTTCAGGAGAACCGCCGCCGC215.384615384615385No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC215.384615384615385Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCA17.6923076923076925No Hit
TTGCCGGTGGTGGTGACTCCGCAACCAAATCTAACTACACAGCGGGTAAA17.6923076923076925No Hit
GCTTGTAGTGAGTTGCGGTCATCTTCCAATTTCTTTACCTGGTCTTCCAG17.6923076923076925No Hit
CCCTGTGGAGTTGTGCAGTGCACAGCATGCACAACTATATATGGTGGTCC17.6923076923076925No Hit
GTGGCAGTCCTCCTTGAAGAGGTTGCTGATGATGTTGCTGCCCGAGGGAC17.6923076923076925No Hit
ACACAGTTCTTCTCTTTTCCCTTCTTTCTCTAAATGGAAATAATTCTATA17.6923076923076925No Hit
TTGCCGGTCTGGGCGCCGGGCTGGGCCAGCACGGTGCGCAGCGCGGGCGT17.6923076923076925No Hit
GAGTAAGATACTCTTATTTTCACCGTTTTACAGATAAGAAAATTGATCCT17.6923076923076925No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTAAAATCTGCAAGAGGATATT17.6923076923076925Clontech SMART CDS Primer II A (100% over 21bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)