FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length146
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGATCGCGTCGAGCGGCCGGCGCCGGATGCTGGAGCTGGGTAACGCC214.285714285714285No Hit
GGCCTCAGGTGCCTTGGGGTACATTGTCATGCTATAAGGGATGTATATCA214.285714285714285No Hit
GCGCCGCACGTCGGGCCGGAGTTCGTCGTGGTGGTGTGGTGCGTCGGCAT17.142857142857142No Hit
GAACGCAGTCTACACCTTCAGCACTGCGTCGCTGGCGAACCTTAATAACG17.142857142857142No Hit
GGATCGGCGTACTGTCGATAAGGACGTGATCGAATTCCTGCATCAGCTGA17.142857142857142No Hit
CACGCTGGCCGATCGCCCGGAATGCGTCTGGCCCTGCCGGCTGCAGCTCG17.142857142857142No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGTGTACATGGGCTGTCTCTTAT17.142857142857142Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTCGGCTATGCGGCCGGTGCTGTCGTCGCGGGAGCTGGCTTCGAAGGA17.142857142857142No Hit
GGAAACTGAGGCTTAAAGAGGTCTATCAACTTGTCCCACGTTACCTTCTA17.142857142857142No Hit
GGCTCCTGACGTGTATCGAACCTCCACAGCCCAGGCAAGATGTGCTGAAT17.142857142857142No Hit
AGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGC17.142857142857142No Hit
GTATAGGAATGCTTGTGATTTTTGTACATTGATTTTGTATCCTGAGACTT17.142857142857142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)