FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length146
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAGAGCTTCTCCGCGATCGGCATCACCGTGACGTTCAAGGGCGTCGA28.695652173913043No Hit
GGGGAGGGGACGCTGAGCGACGACGAGCTCTTCTGGCTGTCTCTTATACA28.695652173913043No Hit
GGATATGGTAGACTCAAATAGTAAGTTGAGTTCACAGTTCGTGCTGGATT28.695652173913043No Hit
GATGAGGGGCTGGCCTCCCGTGCAGTTCTTGTCGGAGACGTCATGACCGA28.695652173913043No Hit
GCCGGGGGCGGAGGTGTGGACCGTCTTCGCGGGTGTTCCCGCCGACGGCG14.3478260869565215No Hit
AGCTAGAGGCGAGGCGGTAGATCAAACGGCGCTCGGCAAAGGAAGCGGAC14.3478260869565215No Hit
GAGCGGGGCAGCTTCGCCGACTTTGTGGCGGCCCGAGCCTTGTCTGAGAC14.3478260869565215No Hit
GTTGGGGTCTGCTGGTCCTGATTTGCCTTGCTGGTTCGTCGGTGACACAC14.3478260869565215No Hit
CATAGACCCAGATGAGTGAGTCGCTCGTCTCGCCGGACGGTCCGCCCAAG14.3478260869565215No Hit
GATCCCGCCGACGCGTTTGATCTCCACGGCCACGGAGCGGCCCTCGGCGT14.3478260869565215No Hit
GGAGATTGCCACGGAGGCCATCGACCGGCTGCACTCCACCGCCCATTCCC14.3478260869565215No Hit
GTCCTCAATGAGTCCTCTTATTCATGCACCCGGGCTGATCGCCTTCGCCA14.3478260869565215No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA14.3478260869565215Clontech Universal Primer Mix Long (95% over 21bp)
GGACGCGGCTACCCGATTGGCGCAGCGTTCTTCTCTGTTGGTCGTTTTTA14.3478260869565215No Hit
TCTCAGCCACTTGGGAGGCTGAGGCTGGAGGATCACTTGAGCCCAGGAGT14.3478260869565215No Hit
GCAAATATTCCTATGAATCCATCACAATGTTTATGTTTATCGTAAGTGCT14.3478260869565215No Hit
GATTTCTAGTTTGTTCCTCTTCCATCTTCTTCATATTTTTGCGTTTTGTT14.3478260869565215No Hit
GGGCGTCGCGGGGTATGACGTGCGCTCTTGGGCGGGCATCCTGGCGCCGG14.3478260869565215No Hit
GAGCCAAAATTGCGCCACTGCACTCCAGCCTAGCAACAGAGCAAGACTCC14.3478260869565215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)