FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTAGGCTACTGCTCTAAATAAAGCATCGGCAATTTGGTTATCCGATTT216.666666666666664No Hit
TTCAAGACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAATA216.666666666666664No Hit
CGCCGGGGCCGATCAGGTCCTCGAGCTCGACCACCCGGCGGGTGAGCTCG18.333333333333332No Hit
AGGCAGAGGTTGCAGTGAGCCAAGATCTCGCCATTGCACTCCAGCCTGAG18.333333333333332No Hit
GTCCACACTCTGTGAGGCTAGCAGAGGAGACTCAAACATGTAGCTCAGGC18.333333333333332No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTAA18.333333333333332Clontech Universal Primer Mix Long (95% over 21bp)
CACGGCGACCGTCCGACGATCGTCCTGACCGCCTCGTCGGTCCGCGACAC18.333333333333332No Hit
GGAGAAAGTCTAAAGAAAAATGTTGGGTGAGCTGCAGTCACGTAGGGTGG18.333333333333332No Hit
TTCTTCCATAGCACACTTCTAAAATACCTGTTTTATAGGGTTTAAACTAT18.333333333333332No Hit
GGGCTTGGTGACCCAGTCGTCGGCGCCCATGCGCAGCCCACGGACGCGCT18.333333333333332No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)