FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length146
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACTCTTACTTTTCGATTTTATTACCTACCAAGCATTCTGATAT26.451612903225806No Hit
CACTTTGGGGGACCACTTTTGCTGGGCCCGCCCCCATTACTGCTGTTGCT26.451612903225806No Hit
GGATGAGAGAGCAGCCCAGGGGCACGCAGGCCGACCTGTCTTAGAGATCA26.451612903225806No Hit
AATCCGTAAAAAGGAATGAAGTTTGGGAACATGCTACAACATGGATGAAC26.451612903225806No Hit
GCTCTTGAGTCCCTTGACATGGTAACTCCTATCCCTCTCCCATCAGACCA26.451612903225806No Hit
CCATTCTTTCCATTCCATTCAAGTTGAATCCATTCCATTGCATTCCATTC26.451612903225806No Hit
GCCCAGGGTGTCAGTCCACAGTGCTGCCTGGCTGTCGGCAGACCACATCT26.451612903225806No Hit
GTGCAGGGAGGCCCTGAGGACGTGGGCTGCAGTGGGCAGGAGAGGGACTC13.225806451612903No Hit
CTTACCCAAGGGCTGAGCACATGAGAGGAGGGAAGAGTGCATGTGCCTAG13.225806451612903No Hit
GCACCTTCAGAAAAACTCTAGTTATGGGAACTTTTGCTGATATAGGACAT13.225806451612903No Hit
TTTCAGGTGGAACAGTATGGTTGCAGAGAGTCCATTTGGACATAGATACA13.225806451612903No Hit
GGAAAGGACATAACCAAAAATGAAAACTACAGACCAATATCCCTGATGAA13.225806451612903No Hit
ACAAAAATAGGGAGGAGTAGGCTGATAGTGCATGAAAACAGCCTAAGAGT13.225806451612903No Hit
GTATCAGTGTTATTGATAAAAAGCTAGATTTCAGAGTGTGAGATACAGAC13.225806451612903No Hit
ACTCTCTTCAGAACAACCAGGAATGGACAGTTCCATCTCCCTCTCAAGAA13.225806451612903No Hit
GCGTGGGTGACAGAGTGAAATTCCATCCAGAAAAAAAAAAAAAAGAAAGA13.225806451612903No Hit
ATTTATTTATATCAATATTTCTTTTGAGTGCCAAGCAGCCCAGAGATAAA13.225806451612903No Hit
GCCTGAACACCCTCCACTGGGCTGAGAACAACTCTCTGAGACCTCTCTCA13.225806451612903No Hit
CCACTGCACTCCAGCCTGGGTGACGGAGTGAGAACTCTTGTCTCAAAAAA13.225806451612903No Hit
GTGAATTCTCATGTCCTATGAAGAGAAAATAGAAGGAGGAAGGGAGAAAA13.225806451612903No Hit
CGCGCGCACCTCGGCGATGGGGCTGTCGAGGCAGCCCGATTCGTCGAGCT13.225806451612903No Hit
CCACTAAAGAACTTTTCCATGCAACCAAACACCACCTGTTCCCCAAAACT13.225806451612903No Hit
CCCCCCAAAACAGCATGAACAAACGGGGACACCTTTCCCTGCACACAGCA13.225806451612903No Hit
GTAATCCCAGCACTTTGTGAGGCCGAGGCGGGAAGATTGCTTGAACTCAG13.225806451612903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)