FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length146
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGATACCCTTCTGTTTTGTGAGAATTATTGGCTAATCATAGACATGAT36.521739130434782No Hit
ATTTTGATCTTCCAAAAAAACTGTGATAACCTGTTCCAAAGAAATCACAA36.521739130434782No Hit
CTGCCCCAGCTGGTCTGCCGCCTCGTCCTCCGGTGCTCCATGGATGACAG24.3478260869565215No Hit
GGTGAGGAGGGCGGTGTGGGAGCTGAGGCTTCTTTTCAGCCTGGCAGCTG24.3478260869565215No Hit
GTATTAATGCAGTGGCAGCCGCTGCACGCAGACATGAGGGCTAGGCTAAA24.3478260869565215No Hit
CACACACACACACACACAATGATGGAAGTAAATTAGAGGGACATAGCAAC24.3478260869565215No Hit
CACAACCAGAGTGAGAATCATGAAGTCATTGTCTGAGGCTCCAGCAGCTG24.3478260869565215No Hit
CCATCGCCGTCCAGGCGCAAACGATGAGATCGCTGGGCCTGAAGGGGAAA24.3478260869565215No Hit
CAGGCACCTCCTTCACAAGGCAGGAGGAGAGAAGGAGAACAGGAGAAACT24.3478260869565215No Hit
GTTTGCCTCATCCATAAAACAATTTTGAATGCCCTCAACTGTGTGTAAGC24.3478260869565215No Hit
CAGATATATGTGTACTAATATAGACTAGTTACCAAGGTATGCTAGGTGAA24.3478260869565215No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12.1739130434782608No Hit
CACAACTTATCTTTTGATTGTCAAATCATTAATGTTAGTTATCTCTTGTT12.1739130434782608No Hit
GAAGCGAGCGCCGCGCGGTACAGCCACGTGAAGATCGTGCCGTGCAGGCG12.1739130434782608No Hit
GGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACT12.1739130434782608No Hit
AGATTATGGTTATAAATGCTTTGATGTGGTAAACAGAGGGGCTCTGGAAA12.1739130434782608No Hit
GTCTCTCTCTCTCTCTCCCCCCTGTCGGCTGTTTCTCTGTCTCTGTCTGT12.1739130434782608No Hit
TGATTATGGTTATAAATGCTTTGATGTGGTAAACAGAGGGGCTCTGGAAA12.1739130434782608No Hit
CCCTTTTTGCCGATCACGACGCCCGGACGAGCCGAGAAGATCGTGATGCG12.1739130434782608No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA12.1739130434782608No Hit
GCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTC12.1739130434782608No Hit
GGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAACGAG12.1739130434782608No Hit
CTGTGCAGCTGACGAGGAAGGCCGAGCACTCGACAGCGGTATGGCGAAGG12.1739130434782608No Hit
TGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAAATTAAGCCGCAGGC12.1739130434782608No Hit
CCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCG12.1739130434782608No Hit
GCACTTAGTAGCTGTGGAGGAAGATGCAGAGTCAGAAGATGAAGAGGAGG12.1739130434782608No Hit
GACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGC12.1739130434782608No Hit
CGGTGAGGCGGGGGGGCGAGCCCCGAGGGGCTCTCGCTTCTGGCGCCAAG12.1739130434782608No Hit
CCGCACGTTCGTGGGGAACCTGGCGCTAAAAAATTAGTATAAAAACCCAA12.1739130434782608No Hit
CTATGATTTGGGTTAATTCATTTTGTCTTTTCCTAAATTTGCACAATGTT12.1739130434782608No Hit
CTCCTGAAGAGCACGACAGAGACTAGGGTACAAACACAGTAAACTCATCT12.1739130434782608No Hit
CATTCATATGCGTGCTTGGCAGGAGATGTTCAACGAGGAGTTATCGCGGC12.1739130434782608No Hit
GGTCTCTAACTCCTGACCTCAAGCAATCCACCCACCTCAGCCTCCCAAAG12.1739130434782608No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)