FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003650951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length146
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTCTAGGCACGGCGTTTTGCTGATTCAGCGGTAGCAAACTGCTACTG36.976744186046512No Hit
ACACCGCACGGGTGCACGACAGCTCAGGTCCCCCTTATCCCAAAGTTACG24.651162790697675No Hit
GTGCTGGTGTGCGTCGAGCAGGATGCCTCTGGCATCGCCTGGGACCTCAC24.651162790697675No Hit
GGCCCAGATCGTTAGGGTAATTGTGCTGAGCAGTCTGACGCTGCTCCTCG24.651162790697675No Hit
GTGCTGTGGCCGATCTATGACCAGAACGATCTTGGAGGGGTCATCGCGAC24.651162790697675No Hit
CAATATACTTATATGAGTATGCTTAATATGAATATGCTTATTCAAAGCTT24.651162790697675No Hit
CTCGTTACGAGCGTGGACGACCACTTCGTCATGAACCATCACACTCAGGC24.651162790697675No Hit
GTCTACGACCAAACGACGGCGTGGAAATTCCCTAGGGTGTGGTCAGTTGG24.651162790697675No Hit
TGTCCGGGGTGGACTCCATGATGTGGTGCTCTCTGCTCCGTCGGTGCGGG24.651162790697675No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT12.3255813953488373No Hit
CACGAGGAGGCCGCGGCCTTCGCAGCCGCTGCAGAGGCGACCTTGACCGG12.3255813953488373No Hit
GTTCAGCCCCTGCTCGCCAACCTGGCCGACGCAACCCATGATGACGTCCT12.3255813953488373No Hit
TGCTTCCACTCCTCCTCCTTGGCCTCACGCTCGGCGAGAAGTTTCTTTTC12.3255813953488373No Hit
TTAGTGGGTTTTATCCTAGAACCGTGGCTAATCAGACGCGTCAGGTCACT12.3255813953488373No Hit
ATGCAGCGGCTGTTCGAATCGCGCGAGGGCGTGCTGATGCAGCAGCTCGA12.3255813953488373No Hit
GCCATGTCCATTGTCATGGCTGCCACGGGATGCTCTTGTGATGATGCCAT12.3255813953488373No Hit
CCAGTCGACACATAGACCACCGGGGCGTCGGGATGTCGTTCCAGGAGTTT12.3255813953488373No Hit
CCCGGGAGCGTCAGAAGCAGCTCATCGCTGACGCCTCCCACGAACTGCGA12.3255813953488373No Hit
GTCTCATCGTCGCAAACCTCAGTCCGGTTGACGTTGGTCTTCCTGCACCG12.3255813953488373No Hit
GTGCTGACCGGAATCGTGGCCGCCAACCCCGCCAATAGCACGAGGACGAT12.3255813953488373No Hit
CCTCAGCCTGGTTGGTAATCAGGTGGTGAGTGTAAGTGCACAAGGGGGCT12.3255813953488373No Hit
GGGTGTTGATCCGGCCAAAATCGCTATCGGCCATATGCAGCGCAACTGGG12.3255813953488373No Hit
ATTGAGGACTTTAAGGTTATCCTTGACGCAGCCCATGACCGTGGCCTTCT12.3255813953488373No Hit
CCTTAGAGGTCTGGAAGGTCAGGTCAGTCAGAGAGCCGGTCGGGGTCGGA12.3255813953488373No Hit
CACCCCTTGTTTCATTGTGCCTGTTTTCTTTGACTCTCACCGAAGCCTTG12.3255813953488373No Hit
GAGATGTTCCACGGCACAACGGTCACGGCACAACCTCGCCTGCTCAGCTC12.3255813953488373No Hit
GTGTGCCACACGGACTCAATCCGCTCACACCCGCCCTGGGCCTGCGCAAG12.3255813953488373No Hit
TACCGACGCGGGCCCAAAGCAGGCGCAGATAATCATGGATCTCTGTGATG12.3255813953488373No Hit
GTCTTTACCTAACTCCATTCACTCATAGCAGGAATCATTTTCTGATGTAG12.3255813953488373No Hit
GTCCGGGCTGGTCGCGGACCGCGTGTGTAGCTGTGGTCAGCCCCACAGGG12.3255813953488373No Hit
GTGTCGCCGCCGGCGAAGATACCGGGGACGTCGGTCATGAGGGTTGAGGG12.3255813953488373No Hit
GCAGGTAGCCATGAAAGTCTGGCAACCGGCGAATACTTTGGCAAGAGCAT12.3255813953488373No Hit
AGGTGAAAGAATACAGCAATAAATAATGATACAATTCCTATCCTCAAGGA12.3255813953488373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)