FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24
Sequences flagged as poor quality0
Sequence length146
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGATGGTCGAGTAATCTTTTACAAAAATGTCCCTCATGAAAACAAAA312.5No Hit
GTGGTAGAGTACAAGAGAGAATGCAAATTTCGAAGTCCCTGTTGTGAAAT312.5No Hit
GATTGATTCTTTTAAGAAAATATAGCATGCCTGTAATCCCAGCACTTTGG312.5No Hit
GCCTAAAACTAAACTCACTCTTAAAATTTTCTGTAATTAATAAATTTCCC28.333333333333332No Hit
CTTTCACTATACAGGCTGGGAAGTGTGTTGATGACCACTGAGCCAGGCTT28.333333333333332No Hit
GTCTTCGGGTTGGGAGCGGTCGGGCTGATGGCGGTGATGGCGGCGGCGCA14.166666666666666No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC14.166666666666666No Hit
TCTGTATGCTTTAAACAACCTTTAAATTAAAAACTAGTGCTTTTGATTAC14.166666666666666No Hit
GTATCAACGCAGAGTACATGGGACGGGGAATCAGGGTTCGATTCCGGAGA14.166666666666666No Hit
GTTTATAGCACTATAGGAGCAAAACCATGCACAATATTTCAAACATACAC14.166666666666666No Hit
GGCGTTAAACCTTTTTCAAATGCAATGGTTTTTCCTTTAGTCTTTTAATA14.166666666666666No Hit
AAGTGAGGGAGACAAGAAACAGAAGAATTCCTAGGACCAAGTTCTGGGTC14.166666666666666No Hit
ACACGGGCTTCCTGCAGAGCGTCGAGCGCAAGTTCGAAGGCGACTTCAAG14.166666666666666No Hit
GGGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGC14.166666666666666No Hit
GCCCGCCTCGGCCTCCCAAAGTGCTGGGATTTCAGACGTGAGCCACCGCG14.166666666666666No Hit
AGCATATGCTACTGGCAGGATCAACCAGGTAGCATATGCTTGTCTCAAAG14.166666666666666No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)