FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003650964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003650964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTCAGGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCAGGTAGCTGG312.5No Hit
CATACCCTCTCTTCTTTGCGTGGAGCAAGGAAATTCTCTGCGTAGGCTTC312.5No Hit
GATGACACAAAGACAGGAAAGACAGTAAAATGTTAACATGCTTTTCTATT312.5No Hit
GAAAATTTGTGATGTTTGCTTTAAACATTCCTTTTTTGTGTGTGTGACCT28.333333333333332No Hit
GCACATGAGTCCGATGGTGAACACAGCTCCTTGTCCATGTCTGTCGGCCA28.333333333333332No Hit
GTACCGGCCGTGCGTACTTAGACATGCATGGCTTAATCTTTGAGACAAGC14.166666666666666No Hit
GGAGATCAAGACCATCCTGGCTAACCCAGTGAAACCCCGTCTCTACTAAA14.166666666666666No Hit
CAAATATACTGATGAGCTTTTCAGAGCAGCTATTTTATATTCAATTATAA14.166666666666666No Hit
TTCAAAATATGAGGGCGTTTCCCCTTAACTAGGTGATTCCAAAGCCACCT14.166666666666666No Hit
GATTTCAGAACATGGTCTCCGGTTTTCTTCTTGTCTTGGCCACTCTTTCT14.166666666666666No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA14.166666666666666No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC14.166666666666666No Hit
GGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGGTGTAAATCTC14.166666666666666No Hit
GGCAAAGTAGGACTGGAGTACTGAGGGCACCCAGTATCTCAAGTAGAGGG14.166666666666666No Hit
GAACGCCGGGATGACCCATTGCCCAAACTCACGCTTCTTCGGCTCACCCG14.166666666666666No Hit
CCTCGGGGCTGCCGGCGAGTTTCGCCCCGCAGCGCAGCGCCAGTTCTTGC14.166666666666666No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)