FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003651006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003651006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length146
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA33.5294117647058822No Hit
CCGGCACACAGCGCATTGAGCCGCTTGCGCATCGCATCGCCCCTGTCTGC33.5294117647058822No Hit
CCACAACCACTTTGTATCCACTCGGCACATCGACGGTGACACTTTGCGCC33.5294117647058822No Hit
GTGTGGCGCTTGGCGAGGGAGCGCGGTGGTGTGTCGAAGAAGAAGCAGCG22.3529411764705883No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC22.3529411764705883Clontech SMART CDS Primer II A (96% over 26bp)
GGAGAAGAGGAAACACAAGAAGAAACGCCTGGTGCAGAGCCCCAATTCCT22.3529411764705883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG22.3529411764705883No Hit
GGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACATCTGACG22.3529411764705883No Hit
GGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGG22.3529411764705883No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22.3529411764705883Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCTCGTCGCGCGCGCACAAGCGGATGCCCAGGGCACGCCATCACGGCG22.3529411764705883No Hit
CGTCTGAACCATGCTATTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22.3529411764705883No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA22.3529411764705883No Hit
ATCCAAAAGGGCCTGGTTCAGCTTTTTCTCCAGGGCCATGGCAGCTTTCA22.3529411764705883No Hit
GGTCCACCGGCCCCTACATTGCAGATGAGGCTCCCAATGTTGACGTTGGC22.3529411764705883No Hit
GACGACGACTCAGATGTAAGTTTTTATTTTTCAAGACTATTGATGTATTT11.1764705882352942No Hit
TGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTGG11.1764705882352942No Hit
GATTACGCGCGATGCCAACGACGACTGCCCTACGGTGGACCGCGGCTGCC11.1764705882352942No Hit
CTGCAGGAACAAGTGGGCTTGGGGAATTGAATAGCAGAGCTGTCTCTTAT11.1764705882352942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG11.1764705882352942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTT11.1764705882352942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1764705882352942No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAAAACA11.1764705882352942No Hit
ATATGATCCGCCGCCACAGCAGCGGCCAACAGGTCGGCGGCGACCTGTCG11.1764705882352942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT11.1764705882352942No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTGGTTTAA11.1764705882352942No Hit
GTATCAACGCAGAGTACATGGGACGGCGGCCACCCAGATAGACAAATTTT11.1764705882352942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11.1764705882352942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTGA11.1764705882352942No Hit
GTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA11.1764705882352942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.1764705882352942No Hit
GGAGAAGAGCAAACACAAGAAGAAACGCCTGGTGCAGAGCCCCAATACCT11.1764705882352942No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCACGCAAAAAAAAA11.1764705882352942No Hit
GTGCAAATACGCGGCCAATGGCGCGTCCACATCGGCGACAAACCCCGCTT11.1764705882352942No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAACCCCCC11.1764705882352942No Hit
GTTCCAAGTTGAAGGGAAACAAAGAAGTGTTTGATGGTGCCAGAGAGGGG11.1764705882352942No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATTGAAT11.1764705882352942No Hit
GTGCGAGCCTTGCACGAGGTGGTCGGACACCCTGTCGTCAGATGGGTCGA11.1764705882352942No Hit
GGCCAAGCCCGCAGTGCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAG11.1764705882352942No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCAAAAAAAAAAAAAA11.1764705882352942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG11.1764705882352942No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA11.1764705882352942No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAACAATTCACCA11.1764705882352942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG11.1764705882352942No Hit
GAGTACATGGGTTAGGCCCTTGGCACCGAGAATTCCACATAAAAAAAAAA11.1764705882352942No Hit
CATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCC11.1764705882352942No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA11.1764705882352942Clontech SMART CDS Primer II A (96% over 26bp)
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA11.1764705882352942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1764705882352942No Hit
CTACTACATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAAC11.1764705882352942No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1764705882352942No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTAA11.1764705882352942No Hit
TATCAACGCAGAGTACATGGGAGCAGTGGTATCAACCTAAAAAAAAAAAA11.1764705882352942No Hit
CTCTTCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATATGGG11.1764705882352942No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTA11.1764705882352942No Hit
GGACATGGTGGCCGCCAACATCTTCCCCGGCGACATGCTCTGGAAGAACT11.1764705882352942No Hit
CACTGCGGCTGCAGCGTGGTCTCGAAGGCGCCCTGGCGGAAGTCGAACTT11.1764705882352942No Hit
GTATCAACGCAGAGTACATGGGACGGGCGATGGCTTCCGTTGCCCTCGGC11.1764705882352942No Hit
TCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCATTTCC11.1764705882352942No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA11.1764705882352942No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAACACAG11.1764705882352942No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAGTA11.1764705882352942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG11.1764705882352942No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAATAAACCC11.1764705882352942No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAAAAATTAAAAC11.1764705882352942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATA11.1764705882352942No Hit
GTACATGGGAAGCAGTGGTATCAATGCAAAAAAAAAAAAAAAAAAAAAAA11.1764705882352942No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)