FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003651007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003651007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77
Sequences flagged as poor quality0
Sequence length146
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGG33.896103896103896No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC33.896103896103896Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA33.896103896103896No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC33.896103896103896No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA33.896103896103896No Hit
AACCATGGGCACTTTCTTCCAACAGGGTTTGAAAACCATCAACGCTCTAG22.5974025974025974No Hit
GGGGCGGGGCGCCCGCCGAGCCCGGTCACGAACTGCTTTTCGGCGAGCCG22.5974025974025974No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATA22.5974025974025974No Hit
CTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACA22.5974025974025974No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG22.5974025974025974No Hit
GAGTACCTTGTGGGCATTAGGTGATTGTAGTTATACACTTTCACAAAAGA22.5974025974025974No Hit
TATCAACGCAGAGTACATGGGAAGCAGGGTATCAACGCAAAAAAAAAAAA22.5974025974025974No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCTGTCTCTTATAC22.5974025974025974No Hit
GTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACTGTCTCTTATA11.2987012987012987No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGTTAACCAGGGTC11.2987012987012987No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11.2987012987012987No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGTTATCAACGCAGAGTACCTGT11.2987012987012987Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA11.2987012987012987No Hit
GAGCTAATAATGACTGAGATGGAGATATCTCGAGCAGCAGCAGAACGCAG11.2987012987012987No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCAT11.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCTGTCTCTTATACAC11.2987012987012987No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT11.2987012987012987No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTTTAACAA11.2987012987012987No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11.2987012987012987Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAGAAAAAAAAAAAAAAAAAAAAA11.2987012987012987No Hit
GTATCAACGCAGAGTACATGGGGGAGAAGGGTTCCATGTGAACAGCAGTT11.2987012987012987No Hit
TATCAACGCAGAGTACATGGGAAGCCGTGGTATCAACGCAAAAAAAAAAA11.2987012987012987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.2987012987012987No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCATCAACGCAAAAAAAAAAA11.2987012987012987No Hit
ACTAAAAGCCACTCAATTCATGACCTTTAATGGGGGAATCTTACGTGGCG11.2987012987012987No Hit
CACACACACTGCGGCCAGGCATTACGCCAGCTCCCGGATGCCTCAGCCTC11.2987012987012987No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTGATAAAAAAGCC11.2987012987012987No Hit
GCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGTAAAAGA11.2987012987012987No Hit
GTGCGGGGCTGATCCTCACCGAGAGCACCGCGGTCGATCCGCGCGGGCGC11.2987012987012987No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.2987012987012987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGAAAAAAAAAAAAAA11.2987012987012987No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATACCAATGCC11.2987012987012987No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA11.2987012987012987No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2987012987012987No Hit
TCTTCATAGTTCCAGCGGCACTGGTGGTATCCCAAAGAGAAAAGAGGGGG11.2987012987012987No Hit
GCCTTCGGGTCCGCGCACCATCACCGCATAGCCGATCTCGGCGCCCTCGT11.2987012987012987No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACACA11.2987012987012987No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
GTTGAGCAGCAGGTGCATGACGCGGTCGGCATACCAGTTGAAGCTGGTGC11.2987012987012987No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTA11.2987012987012987No Hit
ATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGA11.2987012987012987No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAAACAAAAGCC11.2987012987012987No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCGACGAAAAAAA11.2987012987012987No Hit
GCAGGAAGGGCTCGTCGACCACCTCGATGCCGAGTTCCGGCGGCGGATCG11.2987012987012987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT11.2987012987012987No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGGTATCAACG11.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
ATTCCGGAGCGGATGTTCTGTGCGCTTCGTGCCGGCGAAACGGTCCAGCC11.2987012987012987No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTAC11.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
GGCGTGATCGACTCGGCCGACCTGCCGCTGAACGTGAGCCGCGAGCTGCT11.2987012987012987No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTAAA11.2987012987012987No Hit
GTTTAAGATTAAGAGGCATACACCACTTAGTAAACTAATGAAAGCCTATT11.2987012987012987No Hit
CCGTTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCC11.2987012987012987No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)