FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003651171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003651171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length146
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGCTGTTTCCCAGCAAAGATCAACCTCTGCTGGTCAGGAGGGATGC29.523809523809524No Hit
ATAAGGTGGAGCCTGATGGGCTGAGTGGGCATGGCGTCTCCTGCATGTGG29.523809523809524No Hit
CCTGACCTCAGGTGATCCTCATGCCTCGGCCACCCAAAGTGCTAGGTTTA29.523809523809524No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAGTACTCCTGTCT14.761904761904762Clontech Universal Primer Mix Long (95% over 22bp)
TCTGCTGCTTGATCAGCAGCAGGTAGTTGCGCAGCACGTCATCGAAGCGC14.761904761904762No Hit
TTACAAAGGCAGAGTCATGACCCCTCAATCAATTTCCAGAATTGAGGCAG14.761904761904762No Hit
CCCCTGGAAGCCGACCGACTCCATGCCCGCGAGTCCCAGCTCCTTGACCG14.761904761904762No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14.761904761904762Clontech Universal Primer Mix Long (96% over 26bp)
GTTCAGATTTGATTGGATTCTTAGTAATACCTAAGGAAACCCAATTAAAA14.761904761904762No Hit
GTACAAGGTAGACTTTCTCTTTCGCAGCTTTATCTTGCCGTGGGAGCGGT14.761904761904762No Hit
CCATCGTGCACGTCGCGAGGGAGAACGAGAGAACCGCTGGGTACTACTCG14.761904761904762No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT14.761904761904762No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG14.761904761904762No Hit
ATAACGGACCTGGACTTTCATGCGGCTCAACCCAGCAGCGATTCGAAGGG14.761904761904762No Hit
CCTCGGTTCTACTTTTGCCCGACATCAGTTTTTCATAGGTGTTCAGCTAG14.761904761904762No Hit
GGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTA14.761904761904762No Hit
GTTTCACTAGCCTGTAATTGCTGAAATTGAATCTGTAATTTTTGACTCTG14.761904761904762No Hit
CTTCTGGCGCCAAGCGCCCGGCCGCGCGCCGGCCGGGCGCGACCCGCTCC14.761904761904762No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)