FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003651172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003651172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGTCTGAGGACAAGAAGACTCAGAAAAAGTGAAGAGTAATAGGTCTG29.523809523809524No Hit
CACCTGGTGCTCCGTCTTAGAGGTGGGATGCAGATCTTCGTGAAGACCCT29.523809523809524No Hit
GAAACATTATTTTGCTGTTCTTCATTGATATCTATTTTCAGAGGAATTAT29.523809523809524No Hit
GAGTACTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCCTGTCT14.761904761904762Clontech Universal Primer Mix Long (95% over 21bp)
CCCGTGTTGCGCCCGAGTCGGTGAAGATGCGCGCCGATTTCGACTGGCCG14.761904761904762No Hit
TTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGA14.761904761904762No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG14.761904761904762Clontech Universal Primer Mix Long (96% over 26bp)
CATTTCATCGGCCGTCCGCTGATCGGGCTGAACTTCAGGCAGCAGCGCTA14.761904761904762No Hit
GAGCTACGAAGTTGCCCGTTCCCGGTTACTTCCGGGCTTGCGAGCGACGA14.761904761904762No Hit
GCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCA14.761904761904762No Hit
CCCGCCGGGTCCGCCCCCGGGGCCGCGGTTCCGCGCGGCGCCTCGCCTCG14.761904761904762No Hit
GATTAATATTGGTCGGGTGAGGAATTAAAAACCATTATCGCTAATTCCTA14.761904761904762No Hit
CTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTC14.761904761904762No Hit
GCCGGGCAAGGTGAACTTCGCCTCCGGCGGCAACGGCACCTCCACGCACC14.761904761904762No Hit
ATCCAACTGCACGCCCAATCCAGCACAAGCGCCGATTGCCTGAGCTGTGT14.761904761904762No Hit
CTGCAGCCTCGATCTCCCAGGCTCAAGCGATCCTCCCACATCAGCCTCCC14.761904761904762No Hit
GTAGTCTGCAAAATGTCTGATCATTTACCTTTTCCCAATGCACAGTTACC14.761904761904762No Hit
GCTAAACAGAGAGCTGGAAGAAATGAGGGTTACCTATGGGACTGAAGGAC14.761904761904762No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)