FastQCFastQC Report
Tue 10 May 2022
EGAF00003651361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003651361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16941
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA1580.9326486039785137No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1020.6020896051000532No Hit
GTACATGGGAGATCATCAGCTACGGCACCTCGAGCTACGGCTACGACATC1000.5902839265686796No Hit
GTCCAGACCACTTCGGTGTTCTTGAGCACGGTGGAGACGAAGGCCGCTTC530.3128504810814002No Hit
GTATCAACGCAGAGTACATGGGAGATCATCAGCTACGGCACCTCGAGCTA350.20659937429903782No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA340.20069653503335103No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT320.18889085650197746No Hit
GCCTGGGAGCGCTCAGGTCTTCGGCAGCGTGACGCCGCGCTGGCCCTGGT310.18298801723629066No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA270.1593766601735435Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC250.1475709816421699No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA250.1475709816421699No Hit
GTACATGGGAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTT230.1357653031107963No Hit
CCACAGCACGGTGGTCGACCCGAAGAACTTCGACGAGAAGAGCTTCGTCG220.1298624638451095No Hit
CTTGAGCACGGTGGAGACGAAGGCCGCTTCGCGGTCGTTCGACGGCGGCC210.12395962457942271No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG210.12395962457942271No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT210.12395962457942271Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAGATCATCAGCTACGGCACCTCGAGCTAC210.12395962457942271No Hit
CTCCTTGCCGGCCGTTGGAGGAGGAGTTGCCGGCGGTCACGGGATCGTAC210.12395962457942271No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG200.11805678531373591No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA200.11805678531373591No Hit
CCTTGTAGCTGGTCTCGCAGACCTCGTCGCTCTCGAAGAACAGCACCTGC200.11805678531373591No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA200.11805678531373591No Hit
TCCTTGGCATACCCGCTTACAGACGCGGTCATCTTCCGAAGTACGATCCC190.11215394604804911No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT190.11215394604804911No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG180.10625110678236231No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT180.10625110678236231No Hit
AGTAAGAGGTGAGGCCACGGTTGACACCCAGGCAGGCTGGCTCCAGTGCC180.10625110678236231No Hit
GCCCTCGCCCGCGTAGATCTTGGCGGGTAGCGGCGTGGTGTTGCTGAATT180.10625110678236231No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA170.10034826751667551No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG170.10034826751667551No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG170.10034826751667551No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)