FastQCFastQC Report
Tue 10 May 2022
EGAF00003651391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003651391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324502
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG26250.8089318401735582No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG19980.6157126920635312No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA17580.5417532095333774No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC16360.5041571392472157No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG15030.4631712593450888No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT14310.44098341458604257No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT11850.36517494499263486No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC11300.3482258969128079No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG10800.3328176713856925No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA10300.31740944585857717No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC10260.3161767878164079No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG9770.3010767267998348No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG9430.2905991334413964No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC9330.2875174883359733No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT8840.2724174273194002No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA8190.2523867341341502No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG8090.2493050890287271No Hit
CCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGG7390.22773357329076552No Hit
GTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTA6310.1944518061521963No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC6180.19044566751514627No Hit
GTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACC6130.18890484496243476No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTAC5850.18027623866725012No Hit
CTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATA5800.17873541611453858No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA5780.17811908709345398No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG5670.1747292774774886No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG5660.17442111296694626No Hit
CACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCC5650.17411294845640396No Hit
CTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGC5420.1670251647139309No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA5350.16486801314013472No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA5270.16240269705579627No Hit
GTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT5270.16240269705579627No Hit
GCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAAC5230.16117003901362703No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG5190.1599373809714578No Hit
CTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGA5170.1593210519503732No Hit
GGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA5080.1565475713554924No Hit
CCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAG5030.15500674880278087No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCATTCCAGTGAG4880.15038428114464625No Hit
GTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGC4810.1482271295708501No Hit
CTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAG4690.14452915544434242No Hit
TCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCC4680.14422099093380011No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC4650.14329649740217318No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT4590.14144751033891934No Hit
TGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATG4550.1402148522967501No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT4490.13836586523349625No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA4460.13744137170186932No Hit
GTTCTGTAATCGATAAACCCCGATCAACCTCACCACCCCTTGCTCAGCCT4430.1365168781702424No Hit
GCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA4410.13590054914915778No Hit
GGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAG4360.13435972659644624No Hit
TATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACT4360.13435972659644624No Hit
TAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTG4170.1285046008961424No Hit
GTACATGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT4170.1285046008961424No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA4140.12758010736451547No Hit
GCGGTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGA4120.12696377834343087No Hit
CTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC4110.12665561383288856No Hit
GATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCA4040.12449846225909239No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA4030.12419029774855007No Hit
GGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTG3990.12295763970638085No Hit
CAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACA3990.12295763970638085No Hit
GCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAA3980.12264947519583853No Hit
CTCATGGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTA3920.1208004881325847No Hit
CGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACC3900.12018415911150009No Hit
TAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACC3880.11956783009041547No Hit
CCTCATGGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTT3850.11864333655878855No Hit
TAGTAAGATTACACATGCAAGCATCCCCATTCCAGTGAGTTCACCCTCTA3840.11833517204824623No Hit
GAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCC3810.11741067851661932No Hit
GCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATC3790.11679434949553469No Hit
GGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT3710.11432903341119624No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC3650.1124800463479424No Hit
GCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCC3600.11093922379523086No Hit
CCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACAC3600.11093922379523086No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC3530.10878207222143468No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA3530.10878207222143468No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT3510.10816574320035006No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG3500.10785757868980778No Hit
ATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTCTGGGG3500.10785757868980778No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT3490.10754941417926546No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT3490.10754941417926546No Hit
ATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGA3480.10724124966872316No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA3430.10570042711601162No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT3390.10446776907384239No Hit
ACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAG3370.10385144005275776No Hit
GATTACACATGCAAGCATCCCCATTCCAGTGAGTTCACCCTCTAAATCAC3310.10200245298950392No Hit
GCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTC3280.101077959457877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)