FastQCFastQC Report
Tue 10 May 2022
EGAF00003651945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003651945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503683
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC17410.3456539132748177No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12020.23864216183591666No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC10650.21144251443864495No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA9330.18523555490258753No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC9320.18503701733034467No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT9140.18146334102997322No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT8520.16915401155091597No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT7930.15744029478858726No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA7610.1510870924768158No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7380.14652072831523No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT7330.1455280404540157No Hit
GTACATGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTA7020.1393733757144871No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA7010.1391748381422442No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA6950.1379836127087871No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG6860.13619677455860135No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT6580.1306377225358013No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6500.1290494219578584No Hit
GGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCG6410.1272625838076727No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA6140.12190206935711548No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC6110.12130645664038692No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG6100.12110791906814405No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC6070.12051230635141547No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT6050.12011523120692975No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG6040.11991669363468689No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA5950.11812985548450117No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG5790.11495325432861542No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT5760.11435764161188684No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT5720.11356349132291542No Hit
GTATAAGAGATCAGGTTCGTCCTTTAGTGTTGTGTATGGTTATCATTTGT5680.11276934103394397No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT5640.11197519074497254No Hit
GTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAATAAAA5550.1101883525947868No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG5520.10959273987805822No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT5490.10899712716132964No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC5480.10879858958908678No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT5480.10879858958908678No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT5460.10840151444460107No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA5460.10840151444460107No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC5340.10601906357768677No Hit
GCAGTAATGTTAGCGGTTAGGCGTACGGCTAGGGCTATTGGTTGAATGAG5320.10562198843320103No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA5250.10423222542750103No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC5200.10323953756628673No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT5190.10304099999404387No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC5090.10105562427161528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)