FastQCFastQC Report
Tue 10 May 2022
EGAF00003651946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003651946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503683
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC17960.35657347974817494No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13180.2616725202160883No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC9560.1898019190641733No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA9350.18563263004707325No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT8960.17788966472960174No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC8850.1757057514349303No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT8710.17292622542353028No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7670.15227831791027294No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT7640.15168270519354435No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA7130.14155728900915854No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6840.13579969941411563No Hit
GTACATGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTA6810.13520408669738704No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC6790.1348070115529013No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT6750.1340128612639299No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA6660.13222602311374418No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT6500.1290494219578584No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG6480.1286523468133727No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG6450.12805673409664411No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6450.12805673409664411No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6410.1272625838076727No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT6370.12646843351870127No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA6280.12468159536851553No Hit
GGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCG6170.12249768207384405No Hit
GTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAATAAAA6160.1222991445016012No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT6110.12130645664038692No Hit
GCAGTAATGTTAGCGGTTAGGCGTACGGCTAGGGCTATTGGTTGAATGAG6010.11932108091795833No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC5860.11634301733431543No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT5850.11614447976207257No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT5850.11614447976207257No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA5750.11415910403964398No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG5680.11276934103394397No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA5670.1125708034617011No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT5490.10899712716132964No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA5300.10522491328871532No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC5240.10403368785525816No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC5220.10363661271077244No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC5120.10165123698834386No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT5120.10165123698834386No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT5060.1004600115548867No Hit
GTATAAGAGATCAGGTTCGTCCTTTAGTGTTGTGTATGGTTATCATTTGT5040.10006293641040098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)