FastQCFastQC Report
Tue 10 May 2022
EGAF00003651948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003651948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508133
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC17910.3524667754308419No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13020.2562321282026556No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC9700.1908949034996743No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA9190.18085816115072234No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT9060.17829977584608753No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT9050.17810297697650027No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC8780.17278940749764332No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT8500.16727903914919914No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA7620.14996073862551731No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7480.14720555445129524No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA7260.1428759793203748No Hit
GTACATGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTA7100.1397271974069781No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6950.1367752143631687No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6730.13244563923224825No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT6560.12910005844926428No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA6540.12870646071008968No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG6520.1283128629709151No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG6470.12732886862297862No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT6330.12457368444875652No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG6320.12437688557916925No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT6270.12339289123123277No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT6220.1224088968832963No Hit
GGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCG6110.12024410931783606No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6030.11866971836113774No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA6010.11827612062196315No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA5990.11788252288278855No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC5910.1163081319260902No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT5890.11591453418691564No Hit
GTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAATAAAA5820.11453694209980457No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC5780.1137497466214554No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC5740.11296255114310624No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT5740.11296255114310624No Hit
GCAGTAATGTTAGCGGTTAGGCGTACGGCTAGGGCTATTGGTTGAATGAG5470.10764898166424931No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA5420.10666498731631287No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT5380.10587779183796368No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG5310.10450019975085263No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT5270.10371300427250345No Hit
GTATAAGAGATCAGGTTCGTCCTTTAGTGTTGTGTATGGTTATCATTTGT5250.10331940653332888No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT5240.10312260766374157No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT5180.10194181444621782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)