FastQCFastQC Report
Tue 10 May 2022
EGAF00003652276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93861
Sequences flagged as poor quality0
Sequence length146
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC6130.6530934040762404No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4920.5241793716239972No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC4450.4741053259607292No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA3540.3771534503148273No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA2800.29831346352585203No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT2780.29618265307209596No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT2650.28233238512268144No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG2600.2770053589882912No Hit
GTCTATACATGGACGACACTTTGCACATCATAGTGCGACGTTGATCCAGA2570.27380914330765704No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC2460.2620896858119986No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG2460.2620896858119986No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG2390.2546318492238523No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC2390.2546318492238523No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG2250.2397161760475597No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA2250.2397161760475597No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT2230.23758536559380358No Hit
TCCTTATACAGTACGCAGCGACCGTAACGCTGTATGAGATCGCCGCATCC2120.22586590809814514No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT2060.21947347673687687No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA2030.21627726105624273No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1990.2120156401487306No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT1950.2077540192412184No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1940.20668861401434033No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1940.20668861401434033No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA1870.19923077742619405No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG1860.19816537219931601No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG1820.19390375129180384No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG1820.19390375129180384No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT1760.18751131993053557No Hit
TGTATAGACAGTGCGCGTATCATATAGGATGCGGCGATCTCATACAGCGT1740.1853805094767795No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA1620.17259564675424297No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG1610.17153024152736493No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC1590.16939943107360883No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT1590.16939943107360883No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG1570.16726862061985276No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1520.16194159448546255No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA1490.15874537880482842No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA1450.15448375789731625No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC1440.1534183526704382No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG1430.15235294744356015No Hit
CATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCAT1400.14915673176292601No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA1360.14489511085541384No Hit
CTATAAGCGCTGTCACGGATGTATAAATCGCTCGAGAATCACACACAGTC1360.14489511085541384No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA1350.1438297056285358No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA1330.1416988951747797No Hit
CACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCT1320.14063348994790167No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC1280.1363718690403895No Hit
CCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGT1250.1331756533597554No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC1240.13211024813287736No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA1240.13211024813287736No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG1220.12997943767912126No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG1220.12997943767912126No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT1220.12997943767912126No Hit
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT1210.12891403245224323No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG1200.12784862722536516No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG1200.12784862722536516No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC1190.12678322199848713No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG1180.1257178167716091No Hit
ATTCTGTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGT1160.123587006317853No Hit
CTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCG1140.12145619586409692No Hit
TTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGA1130.12039079063721886No Hit
AACATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCC1120.11932538541034082No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG1120.11932538541034082No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT1120.11932538541034082No Hit
GTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAGTG1110.11825998018346279No Hit
AACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC1100.11719457495658472No Hit
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGC1100.11719457495658472No Hit
ATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGA1100.11719457495658472No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC1090.11612916972970669No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC1090.11612916972970669No Hit
CCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCTAT1080.11506376450282865No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA1070.1139983592759506No Hit
AACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTG1060.11293295404907257No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT1050.11186754882219453No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA1050.11186754882219453No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG1030.10973673836843843No Hit
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC1010.10760592791468236No Hit
GTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACG1010.10760592791468236No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC1000.1065405226878043No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG990.10547511746092626No Hit
GTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAACA990.10547511746092626No Hit
GTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTT980.10440971223404823No Hit
AACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGA980.10440971223404823No Hit
GCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTG980.10440971223404823No Hit
ATTGTGAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCA970.10334430700717016No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC970.10334430700717016No Hit
GCTTAGAGCGTATCTTCCGGGATCGGGCAAAGGGGCAACTCGAGCTAATC970.10334430700717016No Hit
GTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTC950.10121349655341409No Hit
GCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGAT940.10014809132653604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)