FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA262.2298456260720414Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT262.2298456260720414Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG161.3722126929674099Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG131.1149228130360207Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG110.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT100.8576329331046313Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC90.7718696397941681Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT80.6861063464837049Clontech Universal Primer Mix Long (100% over 23bp)
GCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA80.6861063464837049No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG80.6861063464837049Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC80.6861063464837049Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT70.6003430531732418Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC70.6003430531732418Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA60.5145797598627788Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT60.5145797598627788Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT60.5145797598627788Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA50.42881646655231564Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT50.42881646655231564Clontech SMART CDS Primer II A (100% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50.42881646655231564Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.42881646655231564No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA40.34305317324185247Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT40.34305317324185247Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC40.34305317324185247Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC40.34305317324185247Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA30.2572898799313894No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30.2572898799313894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC30.2572898799313894Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30.2572898799313894Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC30.2572898799313894Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT30.2572898799313894Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG30.2572898799313894Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2572898799313894No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.2572898799313894Clontech SMART CDS Primer II A (96% over 26bp)
ACATGGGAAGCAGTAGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA20.17152658662092624No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20.17152658662092624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT20.17152658662092624No Hit
GGCCTGCGCCGCGGATTCGACCTTTTGCAGGCCGACGCGTGCATGGGTAT20.17152658662092624No Hit
CATGGGAAGAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATACACAT20.17152658662092624Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTAT20.17152658662092624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGAAGAATAGCTGAAGAAAGTTGTCTACTCTCTCAAGGTGGCTGAGGGT20.17152658662092624No Hit
GTACATGGGCATACCGGACTCATCCTGTTTAGAGATCGGAAGAGCGTCGT20.17152658662092624No Hit
GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG20.17152658662092624No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
CTTTTTTGTTATTGTTATGCCACCATACCATTTTGTTAAAATGTTTTCTT20.17152658662092624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT20.17152658662092624No Hit
GTACATGGGGAAGGCTGTATTTGTAGTAGTACTCAAGAATCACCTGATGT20.17152658662092624No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA20.17152658662092624No Hit
CGATGTGTATTTCTCGAAAAAGTACCTCTCGAATGAGTTCAACATCCCCA20.17152658662092624No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20.17152658662092624No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTGATACCACTGCTTCCC20.17152658662092624No Hit
TCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGGACTA20.17152658662092624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT20.17152658662092624No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACAC20.17152658662092624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT20.17152658662092624No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20.17152658662092624Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT20.17152658662092624Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT20.17152658662092624No Hit
GGTGACGCCATCACCTGAATCCAGGACGATGCCTGTCGTGCGGCCAGAGG20.17152658662092624No Hit
CTCCAAGGCTAAATACTCATCATTGACCGATAGTGAACTAGTACCGTGAG20.17152658662092624No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCT20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
ATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACAT20.17152658662092624No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.17152658662092624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAACTAAGCGA20.17152658662092624No Hit
CCCGACGGCCTTGCCCCATGCCAGAGAAAAAAGGCTACTCCAGCGAAAAG20.17152658662092624No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
GCCCGGCATCGCGATCGGCGTCGGCGTCAGGCCGGTGCGCAGGTAGGTGT20.17152658662092624No Hit
CTCTAGGAGGGCTGGCAACTTAGAGGTGGGGAGCAGAGAATTCTCTTATC20.17152658662092624No Hit
GGCTGTAGTGAAGTGGCGTGATCTCGGCTCACTGCCTCCCGAGTGGCTGG20.17152658662092624No Hit
ATCAACGCAGAGTACATGGGCCTGTTGGTACCAAAAAAGGGATAGATCGG20.17152658662092624No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
TTTTTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA20.17152658662092624Clontech Universal Primer Mix Long (95% over 21bp)
GTGTAGGGAAATGTACTGCCCCCATGTACTCTGCGTTGATACCACTGCTT20.17152658662092624Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
CCGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCACAGATGGTAG20.17152658662092624No Hit
GTCGTACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCTTGCACCTG20.17152658662092624No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC20.17152658662092624Clontech Universal Primer Mix Long (96% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.17152658662092624Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT20.17152658662092624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCGAACCCTTCAACTGCTGACTCTCTAGCCCATGAGCCTGTCCACGC20.17152658662092624No Hit
ATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACA20.17152658662092624No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTGTAGGGAAAGAGTGTAGATCTCGTGGCGCCTACATCCCTGTCTCT20.17152658662092624Illumina Single End PCR Primer 1 (96% over 28bp)
GAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAA20.17152658662092624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.17152658662092624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.17152658662092624No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT20.17152658662092624Clontech SMART CDS Primer II A (100% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17152658662092624No Hit
ACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17152658662092624No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)