FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT292.4871355060034306Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA181.5437392795883362Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC131.1149228130360207Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG100.8576329331046313Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG100.8576329331046313Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC90.7718696397941681Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT90.7718696397941681Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT90.7718696397941681Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA80.6861063464837049Clontech Universal Primer Mix Long (95% over 21bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC70.6003430531732418Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT70.6003430531732418Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC70.6003430531732418Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC60.5145797598627788Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.5145797598627788No Hit
GCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA50.42881646655231564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.42881646655231564No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTA40.34305317324185247No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT40.34305317324185247Clontech SMART CDS Primer II A (100% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG40.34305317324185247Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG40.34305317324185247No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG40.34305317324185247Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT40.34305317324185247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA40.34305317324185247Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT40.34305317324185247Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC40.34305317324185247Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40.34305317324185247Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA40.34305317324185247Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG40.34305317324185247No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30.2572898799313894Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATAC30.2572898799313894Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG30.2572898799313894No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT30.2572898799313894Clontech SMART CDS Primer II A (100% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC30.2572898799313894Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT30.2572898799313894Clontech Universal Primer Mix Long (96% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.2572898799313894No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA30.2572898799313894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT30.2572898799313894No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC30.2572898799313894Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG30.2572898799313894Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA30.2572898799313894No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30.2572898799313894Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT30.2572898799313894Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30.2572898799313894Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA30.2572898799313894Clontech Universal Primer Mix Long (95% over 22bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATCTGTCTCTTATACAC20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
CACTCGATGTCGATCAGCGTCGAGTCCATGTCGAAGCCGATCAGCCGGAA20.17152658662092624No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA20.17152658662092624No Hit
CATGTACTCTGCGTTGATACCACTCTTCCCATGCTGTCTCTTATACACAT20.17152658662092624Clontech SMART CDS Primer II A (95% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.17152658662092624Clontech Universal Primer Mix Long (100% over 23bp)
GTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATTCTG20.17152658662092624No Hit
CTCTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAAGAGCGTCGTGTAG20.17152658662092624Illumina Single End PCR Primer 1 (100% over 22bp)
CTTTTTAAAAGATCGCCTTCAAATTATTTTAATCACCTACAACTTTTAAA20.17152658662092624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20.17152658662092624No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA20.17152658662092624Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20.17152658662092624Clontech Universal Primer Mix Long (96% over 25bp)
GGGTGCGCCCATCTTCCAGCTGCTTTCCGGCAAAGATCAACCTCTGCTGG20.17152658662092624No Hit
GTAAGGAGCTGAGTTCTTAAAGACTGAAGACAGGCTATTCTCTGGAGAAA20.17152658662092624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT20.17152658662092624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT20.17152658662092624No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.17152658662092624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTGGGCGAGTCCTTCGACGGCAACCTGCGCAAGCGCGACCTGCAGGCC20.17152658662092624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT20.17152658662092624No Hit
TTTCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGTACCAACAGGCC20.17152658662092624Illumina Single End PCR Primer 1 (100% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC20.17152658662092624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGACGTTGGTGTT20.17152658662092624No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20.17152658662092624Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20.17152658662092624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT20.17152658662092624No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA20.17152658662092624No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
CAATTCTCCCTGCGTCATGGATTTCAAGGTCTTTTAATCACCTTCGGTTT20.17152658662092624No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17152658662092624No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17152658662092624No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
GGATGTAGGCGCCACGAGATCTACACTCTTTCCCTACACGACCTGTCTCT20.17152658662092624Illumina Single End PCR Primer 1 (96% over 29bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.17152658662092624No Hit
AAATATTATTTCACACTAAATGAAACCAGAGAGTTTTGGGAAAAGGTTGA20.17152658662092624No Hit
GTACAGGACCTTTTCGCTGGAGTAGCCTTTTTTCTCTGGCATGGGGCAAG20.17152658662092624No Hit
TATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATG20.17152658662092624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC20.17152658662092624No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC20.17152658662092624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATAGTGAACACTATTCTATTCTGATATAATTCCTTGAAGACAGATTTT20.17152658662092624No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAA20.17152658662092624No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGACTGTCT20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAAAGTTACAAAAAATGTCGTTGAAGAATAATATATTAAAACTGTGG20.17152658662092624No Hit
GCAGAGTACATGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA20.17152658662092624No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
GCCCGCGTCAGTGCCGGGCGCGTCCTGGTCATAGGCGCCGGCGGGCTGGG20.17152658662092624No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.17152658662092624No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC20.17152658662092624No Hit
GTTAACTTATGCACGCTTAACTATCTTAACAAGCTTTGAGTGCAAGAGAT20.17152658662092624No Hit
TCAACGCAGAGTACATGGGGATGTTGAACTCATTCGAGAGGTACTTTTTC20.17152658662092624No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.17152658662092624Clontech Universal Primer Mix Long (96% over 26bp)
TTATAACATCCAGCCAAGAATACAAACACAAAATAACTCTTAATTTAAGG20.17152658662092624No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT20.17152658662092624Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.17152658662092624No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20.17152658662092624Clontech SMART CDS Primer II A (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)