FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA292.2816679779701023Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG272.1243115656963023Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT201.5735641227380015Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG201.5735641227380015Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA131.0228166797797011Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC120.9441384736428009Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG110.865460267505901Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT80.6294256490952006Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC70.5507474429583006Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC70.5507474429583006Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG70.5507474429583006Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC70.5507474429583006Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT60.47206923682140045Clontech SMART CDS Primer II A (100% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT60.47206923682140045Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG60.47206923682140045Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG60.47206923682140045Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT60.47206923682140045Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC50.3933910306845004Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.3933910306845004Clontech Universal Primer Mix Long (95% over 22bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC50.3933910306845004Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG40.3147128245476003Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT40.3147128245476003Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC40.3147128245476003Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA40.3147128245476003Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT40.3147128245476003Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA40.3147128245476003Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT40.3147128245476003Clontech SMART CDS Primer II A (100% over 26bp)
CTGCTTTCACTGCATTCCACAAACGTTGATACGTTGTTTTCACCTTCATT30.23603461841070023No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG30.23603461841070023Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.23603461841070023Clontech Universal Primer Mix Long (100% over 23bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT30.23603461841070023Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC30.23603461841070023Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA30.23603461841070023Clontech Universal Primer Mix Long (95% over 21bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC30.23603461841070023Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA30.23603461841070023Illumina Single End PCR Primer 1 (100% over 21bp)
GTAAAAAGAAAGGTTCATCTCTGTTAGTTGAATACACACATCACAAACAA30.23603461841070023No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.23603461841070023Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT30.23603461841070023Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTGTAATCTTAGCACTTTGGGAGGCCGAGGCAGAAGGATTGCTTGAGAA30.23603461841070023No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20.15735641227380015No Hit
GTCGTGTAGGGAAAGAGTGTAGATCTCGGGGCGGTTATCCCATGTACTCT20.15735641227380015Illumina Single End PCR Primer 1 (96% over 31bp)
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT20.15735641227380015No Hit
GCAGTGGTATCAACGCAGAGTACATGGGTGTACGCGACCCGAGATCTACA20.15735641227380015Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TTCCTGATTTTTTAACTTAGATTTCTCACTAAGTTTCCTGAGTTATTAGT20.15735641227380015No Hit
GCATCGATGTCGAGGACCAATTCCCTGGGCGCCTCGGCGTGGCTGGCAAT20.15735641227380015No Hit
GAGTATAAGAACTTGGAATACTATACACACATTTGCTCTTCCTGAGGACT20.15735641227380015No Hit
GTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGG20.15735641227380015Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTCCTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTC20.15735641227380015No Hit
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG20.15735641227380015No Hit
AAAAAAGCAAAGAAAATAGTTGATACGCGAAAAAAAAAAAAAAAAAAAAA20.15735641227380015No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT20.15735641227380015Clontech SMART CDS Primer II A (100% over 26bp)
GCTGACCACAAGGTGACTTCAAGTGTTATTTAAAGAAAGGAAACATACCC20.15735641227380015No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCA20.15735641227380015No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAC20.15735641227380015No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA20.15735641227380015No Hit
GTCACGGTCTTGTGCTCTAAGGAAAACGACAGCACGTGTTCTTTTTCACT20.15735641227380015No Hit
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCGACA20.15735641227380015Illumina Single End PCR Primer 1 (100% over 38bp)
GAATTATATAATTCTAACGCTTAAATCATGTGAAAGGGTTGCTGCTGTCA20.15735641227380015No Hit
GATATGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA20.15735641227380015No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA20.15735641227380015Clontech Universal Primer Mix Long (95% over 22bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20.15735641227380015Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGAGTTTAGAGAT20.15735641227380015Illumina Single End PCR Primer 1 (97% over 40bp)
TTTCGGGATGGCTCTGTTGGAATGCGCGACCTTTTCGGGCAGCCACTTCA20.15735641227380015No Hit
GCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCC20.15735641227380015No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGATACCACTGCTTCCCATGTAC20.15735641227380015Clontech Universal Primer Mix Long (95% over 21bp)
CACCCTCAACTCTAGAGGATGATTTGGAAGAGACACAGGGAGATCAGAAC20.15735641227380015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACC20.15735641227380015No Hit
GAACGATCAGACAAGCTTTACATTCTACCTATGGTGTTCAGAAATTGAGG20.15735641227380015No Hit
GCCCCAACTCGTGCGCCCAGCACCAGATCGGCGACATCGGCCTGGCCGGC20.15735641227380015No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT20.15735641227380015Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCTGGACTGGTACATCAGCGTGATCAGCGCGCAGCGCGAGATCAGCTC20.15735641227380015No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTCTTATACA20.15735641227380015No Hit
CTATGTGCCAGAGACTGTGCTGTACACTTGACATGTATCATTTCACTTGG20.15735641227380015No Hit
TTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTT20.15735641227380015No Hit
GCCCGACGACGCGCGCGTGCGCCGCGCACTCGCCGGCGAGCATCCGATGC20.15735641227380015No Hit
CTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGG20.15735641227380015No Hit
GCAACTACCTTCCTACACGACGCTCTTCCGACTCTGTCCCAAGTTCTGCA20.15735641227380015Illumina Single End PCR Primer 1 (95% over 22bp)
GGTGCAGACCACACCAGCGTCTCTCTCGTGCCTGCAGTTGCTCTTCAGCC20.15735641227380015No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.15735641227380015Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC20.15735641227380015Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTAT20.15735641227380015Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATA20.15735641227380015No Hit
CGTCAGATGTGTATAAAAAAAAAAAAAAAAAAAAAAAAGTACCTGTCTCT20.15735641227380015No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTA20.15735641227380015No Hit
AAACAAGGATGCTCAAGGACTCTGGACTTGGGACGATGTTGAAAAACCTT20.15735641227380015No Hit
ACCTAGCACTATGCCTGGTTTATCATAGGTACTGAATAAGGAGTTAATGT20.15735641227380015No Hit
CAATAGGTACTCCGGGGAAAGGACCCCAAGGAGTCAGACCACAAATGTAT20.15735641227380015No Hit
GATTTGTCCCTGGAGATGTTGACAGGATTCTGTGAAGGTTTGGGTGGCTA20.15735641227380015No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTT20.15735641227380015No Hit
GTGTAGGGAAAGAGTGATGCGCCGATTCCCATGTACTCTGCGTTGATACC20.15735641227380015No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC20.15735641227380015No Hit
GGCTGACAAAATAAATAAATAAATAAAAATAATAATTCCTTTTATAGGCC20.15735641227380015No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA20.15735641227380015Clontech Universal Primer Mix Long (96% over 26bp)
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG20.15735641227380015No Hit
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTT20.15735641227380015Illumina Single End PCR Primer 1 (100% over 38bp)
CCGTACACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.15735641227380015Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG20.15735641227380015Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGTGAACGCGACCACCGAGATCTACACTCTTTCCCT20.15735641227380015Illumina Single End PCR Primer 1 (96% over 30bp)
GACTTCAACACATCAACCTTCCGTCTCAAAAGAAAAGCATCCCATGCACA20.15735641227380015No Hit
ACTCAGATGCTTGTAAAGAGATGGCTTGATGGAGAAGAATCCTTTTCATT20.15735641227380015No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT20.15735641227380015Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACG20.15735641227380015Clontech Universal Primer Mix Long (96% over 26bp)
AGATTCCTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTTGT20.15735641227380015Illumina Single End Sequencing Primer (96% over 31bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT20.15735641227380015Clontech Universal Primer Mix Long (96% over 26bp)
GATCTACACTCTTCCCTACACGACGCTCTTCCGATCTCCCTGCTGGTACC20.15735641227380015Illumina Single End PCR Primer 1 (96% over 27bp)
GACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGGAGT20.15735641227380015No Hit
GTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGG20.15735641227380015No Hit
GTACATGGGAGTCCGACCACCGAGTCTACATCTTTCCCTGTCTCTTATAC20.15735641227380015No Hit
GTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG20.15735641227380015No Hit
ACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGT20.15735641227380015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.15735641227380015No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.15735641227380015Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGGCACAGGTTCCGTGAGTGCACTGGACAGTAGGTCCTGTCTCTTAT20.15735641227380015No Hit
GTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACAT20.15735641227380015Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCT20.15735641227380015Clontech Universal Primer Mix Long (96% over 26bp)
GGAGGCTGCCTGATCCCCAACAGGCATGACAGGCTCCAGGGAGCAATGGG20.15735641227380015No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)