FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003652745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003652745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3500
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA260.7428571428571429Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG140.4Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT110.3142857142857143Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG80.2285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC80.2285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC50.14285714285714285Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG50.14285714285714285No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC40.1142857142857143Clontech Universal Primer Mix Long (96% over 26bp)
GGTGAAAGCAGCCGTCGGACTGGTGCTGCCCGCCAAGCTGCGGGACCTGT40.1142857142857143No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT40.1142857142857143Clontech Universal Primer Mix Long (96% over 26bp)
CTGCCAGGTTGAGCAGGGCCCCTTCCTCCTTGTCCGGGGCCTCCTGCTCT40.1142857142857143No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC40.1142857142857143Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAAAGCCTGCGCTTGTTTGTCCAATCTTCTCCACTCCGGCGATTTCT40.1142857142857143No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC40.1142857142857143Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT40.1142857142857143Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC40.1142857142857143Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTAGAAAGTGACACTGAGTTTAAAAAAAGTGACACCTTTGATTCATG40.1142857142857143No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA40.1142857142857143Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG40.1142857142857143Clontech Universal Primer Mix Long (96% over 26bp)
GAACTATGTCTGGAAGGCTGTGGTCCAAGGCCATTTTTGCTGGCTATAAG40.1142857142857143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1142857142857143No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)